Isolation and Identification of Bacillus thermoamylovorans

This study aimed to identify microbes that could, at high temperatures, efficiently degrade waste materials from livestock and poultry slaughterhouses. A stepwise gradient heating was applied to screen microorganisms and isolated HL8103.Through colony morphology, strain morphology, physiological and biochemical characteristics, 16S rDNA sequence, phylogenetic analysis. At the same time, the strain was tested for its suitability for livestock and poultry slaughterhouse waste. The results identified HL8103 as Bacillus thermoamylovorans, a gram-positive with straight or curved rod-shape bacterium that had near spherical spores, proximal birth, and inflated cysts. Meanwhile, HL8103 was tested for its adaptability on the slaughterhouse waste and found that it could grow well between 75℃ and 85℃ and withstand a temperature as high as 95℃. And, for optimal results, the medium was to be adjusted to pH 6.8-7.2 and fermented for 18 h.