SRAP Analysis on Genetic Diversity of Brassica juncea

An SRAP marker was applied to analyze the genetic diversity of 44 accessions of Brassica juncea(L.). Thirty-one pairs of SRAP primer combinations, which amplified stable bands with clear and higher polymorphism, were selected by a preliminary screening. A total of 286 DNA bands were amplified from the 44 accessions of B. juncea by PCR amplification.Among them, 60.49% or 173 DNA bands were polymorphic.The average number of DNA bands amplified by each pair of primer combination was 9.23. The clustering analysis by NTSYS statistical software showed that the genetic similarity coefficients were in the range of 0.61-0.89 averaging 0.75. At the coefficient of 0.67, the 44 accessions were divided into 4 groups which were identical with the result obtained by the morphological classification.