Expression Stabilities of Three Housekeeping Genes of Anoectochilus roxburghii

Three unigenes of GAPDH (GenBank accession number:MH899010), Actin (GenBank accession number:MH899011) and α-tubulin (GenBank accession number:MH899012) were selected from Anoectochilus roxburghii transcriptome data. Their expression stabilities under various temperatures at different plant growth stages were studied. The results indicated that the expressions of GAPDH and α-tubulin genes varied under the test conditions, but that of Actin in the A. roxburghii stems and leaves was stable and consistent. It appeared that the gene of Actin would be a plausible candidate to be used as a reference in analyzing the expression of functional genes under varied temperatures during different growth stages.