Objective A feeding experiment was conducted in order to study the effects of feeding Schizochytrium on the resistance of fish to bacterial infection.
Methods Six-month adult zebrafishes were fed with diety added 3% Schizochytrium(Group S) for 28 days in a laboratory culture system, and the control with only basic diety(Group C). Then Vibrio vulnificus FJ03-X2 was applied for infection, and the relative survival percent of zebrafishes were analyzed. And RNA was extracted from the kidney tissues and reverse transcribed into cDNA to detect the experison of lysozyme and tnfb.
Results The survival rate of Group S was significantly increased with immune protective rate reaching 42.8%. The mRNA level of Lysozyme in the kidney of zebrafish in Group S was significantly higher than that in the control (P＜0.01), while the mRNA level of TNFb was significantly lower than that in the control group (P＜0.01).
Conclusion As a nutrient additive, Schizochytrium could improve the ability of zebrafish to resist bacterial infection, which provided a theoretical basis for the application of Schizochytrium in fishery industry.
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