• 中文核心期刊
  • CSCD来源期刊
  • 中国科技核心期刊
  • CA、CABI、ZR收录期刊
CHEN R, CHEN J M, WU M J, et al. Cloning and Expression Analysis of OsPLATZ14 Promoter in Rice ( Oryza sativa L.) [J]. Fujian Journal of Agricultural Sciences,2019,34(10):1137−1143.. DOI: 10.19303/j.issn.1008-0384.2019.10.004
Citation: CHEN R, CHEN J M, WU M J, et al. Cloning and Expression Analysis of OsPLATZ14 Promoter in Rice ( Oryza sativa L.) [J]. Fujian Journal of Agricultural Sciences,2019,34(10):1137−1143.. DOI: 10.19303/j.issn.1008-0384.2019.10.004

Cloning and Expression Analysis of OsPLATZ14 Promoter in Rice (Oryza sativa L.)

  •   Objective  In order to further understand the function of OsPLATZ14 and the role of PLATZ transcription factor in rice growth and development, the structure and spatiotemporal expression pattern of pOsPLATZ14 were studied.
      Method  The length of pOsPLATZ14 was predicted by using the online software of BDGP, FPROM, and Cister. The cis-acting regulatory elements of pOsPLATZ14 were analyzed based on the PlantCARE database. pOsPLATZ14 was isolated from the genomic DNA of Nipponbare, then pOsPLATZ14::GUS vector was constructed and applied for the rice callus transformation. Using GUS histochemical assay, the spatiotemporal expression characteristics of pOsPLATZ14 were revealed.
      Result  The pOsPLATZ14 was determined to be 1 899 bp in length containing several cis-elements which were associated with the light signaling, stress and hormone response. The GUS reporter gene driven by the promoter was significantly expressed at the stages of seed germination, the seedling stage of roots, stems and leaves, in the heading period of roots, stems, leaves, flower spikes, florets, leaf angle, stem-stem junctions, root-stem junctions, as well as the seed maturation.
      Conclusion  pOsPLATZ14 was the constitutive promoter and its downstream regulatory gene OsPLATZ14 might play an important role in the growth and development of rice.
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