Identification of CIPK Family in Rice and qRT-PCR Analysis on OsCIPK5 Induced by Magnaporthe oryzae

  Objective  The family of calcineurin B-like protein-interacting protein kinase genes (CIPKs) in rice was studied using the latest sequencing data to further understand the signal transduction involving a variety of kinases in plants in respond to environmental changes.

  Methods  The structures of CIPKs in Arabidopsis thaliana and rice were analyzed. Combining the bioinformatics and qRT-PCR technology, expressions of the CIPK familie and that of the genes induced by M. grisea in Japonica rice cv. 'Yunyin' were compared.

  Results  A total of 31 OsCIPK genes were identified in the rice genome databank. The phylogenetic tree analysis showed that these 31 OsCIPKs could be divided into 5 subfamilies, which had different structural characteristics of exon-intron and UTR. The expression of OsCIPK5, which was selected from a trend cluster of the gene expressiong profile of 'Yunyin' induced by M. grisea, could be induced by M. grisea.

  Conclusions  It appeared that the intron deletion and fragment duplication played an important role in the expansion of OsCIPK family in rice, and that OsCIPK5 expression was induced in 'Yunyin' by M. grisea.