Determination of Lincosamide Antibiotics Residues in Crab Tissues

  Objective  An HPLC-MS/MS method following an optimized solid phase extraction (SPE) procedure was established for the determination of lincosamide antibiotics in crab tissues.

  Method   SPE column was used to separate and purify the substances from crab roe samples. Lincomycin, clindamycin, and pyrimycin were the target antibiotics to be detected by the methodology.

  Result   Among the tested materials for the chemical separation, OASIS^® HLB SPE column exhibited the highest recovery rate on the antibiotics. For the HPLC-MS/MS determination on the antibiotics, the gradient elution on an Infinity Lab poroshell 120 SB-C₁₈ column (100 mm×2.7 mm, 2.7 μm) using 0.1% formic acid for the mobile phase A and methanol for the mobile phase B under the multi-reaction monitoring mode was applied. A measurement linearity within 50 μg·L⁻¹ with a R²＞0.99 was achieved. The assay performed with a recovery rate ranging 80.5%–99%, the relative standard deviation 2.5%–5.2%, the detection limit 0.22–0.35 μg·kg⁻¹, and the quantitative limit 0.72–1.16 μg·kg⁻¹.

  Conclusion   The newly established methodology was stable and reliable in detecting the antibiotics in crab at a reduced cost.