A Preliminary Study on Genetic and Serological Clustering of Pathogenic <i>Pseudomonas plecoglossicida</i> in <i>Larimichthys crocea</i>

XU Binfu; CHEN Xiujin; CHI Hongshu; XU Mengting; CHEN Jia; LIN Nengfeng; GONG Hui

doi:10.19303/j.issn.1008-0384.2022.009.001

XU B F, CHEN X J, CHI H S, et al. A Preliminary Study on Genetic and Serological Clustering of Pathogenic Pseudomonas plecoglossicida in Larimichthys crocea [J]. Fujian Journal of Agricultural Sciences，2022，37（9）：1109−1116. DOI: 10.19303/j.issn.1008-0384.2022.009.001

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A Preliminary Study on Genetic and Serological Clustering of Pathogenic Pseudomonas plecoglossicida in Larimichthys crocea

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Objective Clustering of strains of Pseudomonas plecoglossicida, a major pathogen that causes the serious bacterial visceral white spot disease on Larimichthys crocea in winter and spring, was studied for disease control and epidemic prevention.
Method The 16S rDNA and gyrB of 7 suspected P. plecoglossicida strains collected from different temporal and spatial sources were cloned and sequenced with evolutionary trees constructed. O and H antigen serums of these strains were prepared for agglutination tests.
Result The 7 pathogens could be clustered into a same branch based on their sequences. Their O and H antigen sera agglutinated with those of the standard P. plecoglossicida reference without significant differences on titers, but not with that of P. fluoresenes, Vibrio alginolyticus, or V. harveyi.
Conclusion It appeared that the 7 collected strains of P. plecoglossicida were genetically closely related and might have a common origin.

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A Preliminary Study on Genetic and Serological Clustering of Pathogenic Pseudomonas plecoglossicida in Larimichthys crocea

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