Interacting Proteins with JsMYB305 Transcription Factor in Jasminum sambac

  Objective   Aroma-regulating mechanism of the transcription factor JsMYB305 associated with terpene synthetases gene of jasmine flower was investigated.

  Method   From the jasmine floral petal yeast database a two-hybrid library was constructed using the white buds and fully open flowers. Proteins that interacted with JsMYB305 were identified and verified by the yeast two-hybrid method.

  Result   The full length of JsMYB305 was significantly self-activated, and the fragments of different lengths were amplified for verification. At a coding length less than or equal to 510 bp, JsMYB305 showed no self-activation. Applying a 510 bp pGBKT7-JsMYB305 as a bait, an interacting protein was obtained from the yeast two-hybrid library and identified to be salicylic acid carboxyl methyltransferase (SAMT). The yeast-directed two-hybrid validation indicated that JsMYB305 interacted with JsSAMT.

  Conclusion   Since SAMT presents in the biosynthetic pathway of phenylpropane/propane, the JsMYB305 transcription factor was believed to also relate to the synthesis of similar aromatic substances in jasmine flowers.