Key Techniques for Tissue Culture and Rapid Propagation of Edible Rose Flaming Fire

  Objective  To systematically study the tissue culture and rapid propagation technology of edible rose flaming fire, and establish the tissue culture and rapid propagation technology system to solve the bottleneck problem of seedling industrialized production of edible rose flaming fire.

  Methods  The best disinfectant and treatment time of explants were selected through comparative test; Through orthogonal test, the best plant growth regulators and concentration levels were selected for bud proliferation culture, and the best rooting basic medium, and plant growth regulators and their concentrations were selected.

  Result  (1) The explants treated with 0.1% HgCl₂ for 8 min and then 6% sodium hypochlorite solution for 3–6 min was the best for tissue cluture of flaming fire; (2) The optimum medium formula for bud propagation was MS+6-BA 2.0 mg·L^-1+NAA 0.2 mg·L^-1+TDZ 0.06 mg·L^-1, and the multiplication coefficient was 3.47; (3) The improved rooting medium for bud seedlings was 1/2MS+NAA 0.2 mg·L^-1+IBA 0.01 mg·L^-1, the rooting rate of which was 89.44%, and the root system was strong.

  Conclusion  The tissue culture and rapid propagation technology system of edible rose flaming fire established by the comparative experiment for explant disinfection, and the orthogonal experiment for bud proliferationand rooting culture, can provide technical support for the industrialized production of edible rose seedlings of flaming fire, and promote the popularization and application of edible roses in agriculture.