High-performance Regeneration of Pyrethrum

ZHANG Yiping; ZHANG Lifang; XU Feng; WANG Lihua; SU Yan; YANG Xiumei; JIANG Yalian

doi:10.19303/j.issn.1008-0384.2023.03.011

Volume 38 Issue 3

Mar. 2023

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Article Navigation > Fujian Journal of Agricultural Sciences > 2023 > 38(3): 346-351

ZHANG Y P, ZHANG L F, XU F, et al. High-performance Regeneration of Pyrethrum [J]. Fujian Journal of Agricultural Sciences，2023，38（3）：346−351 doi: 10.19303/j.issn.1008-0384.2023.03.011

Citation:

ZHANG Y P, ZHANG L F, XU F, et al. High-performance Regeneration of Pyrethrum [J]. Fujian Journal of Agricultural Sciences，2023，38（3）：346−351 doi: 10.19303/j.issn.1008-0384.2023.03.011

ZHANG Y P, ZHANG L F, XU F, et al. High-performance Regeneration of Pyrethrum [J]. Fujian Journal of Agricultural Sciences，2023，38（3）：346−351 doi: 10.19303/j.issn.1008-0384.2023.03.011

Citation:

ZHANG Y P, ZHANG L F, XU F, et al. High-performance Regeneration of Pyrethrum [J]. Fujian Journal of Agricultural Sciences，2023，38（3）：346−351 doi: 10.19303/j.issn.1008-0384.2023.03.011

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High-performance Regeneration of Pyrethrum

doi: 10.19303/j.issn.1008-0384.2023.03.011

Flower Research Institute, Yunnan Academy of Agricultural Sciences/National Engineering Research Center for Ornamental Horticulture/Yunnan Key Laboratory of Flower Breeding/Yunnan Flower Engineering Research Center for Flower/ Kunming Key Laboratory of Genetic Improvement of Flowers, Kunming, Yunnan　650205, China

Received Date: 2022-01-16
Accepted Date: 2022-01-16
Rev Recd Date: 2022-04-01

Available Online: 2023-04-14

Publish Date: 2023-03-28

Abstract

Abstract

Objective Appropriate methods for efficient tissue disinfection, explant induction, and culture medium formulation were established for a high-performance program to propagate pyrethrum. Method Unopened flower buds of white flower pyrethrum were sterilized and used as explants on an MS solid medium for the experiment. Effects of various plant growth regulators on bud induction, proliferation, and rooting of the seedlings were monitored. Result The optimum conditions for disinfecting the explants were found to be a treatment of 75% alcohol for 30 s followed by one of 0.10% mercuric chloride solution for 10 min and another of 15% hypochlorite for 15 min. For bud induction, the choice medium was formulated with MS + 2.0 mg·L⁻¹ 6-BA + 0.5 mg·L⁻¹ TDZ+ 0.2 mg·L⁻¹ IBA; for bud proliferation, MS + 1.0 mg·L⁻¹ 6-BA + 0.1 mg·L⁻¹TDZ + 0.1 mg·L⁻¹ IBA; for rooting, MS + 0.1 mg·L⁻¹ IAA + 0.1 mg·L⁻¹ IBA; and for transplanting seedlings, peat:perlite at 6:1. A survival rate greater than 90% as well as adequate transplanting was achieved. Conclusion The newly developed in vitro regeneration system materially lessened the pressure of the recently encountered white flower pyrethrum germplasm degradation. In addition, a supply of high-quality seedlings could be assured with the proposed propagation program.
- White flower pyrethrum,
- adventitious bud,
- regeneration system

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References(15)

References

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