Objective Family of aminoacylase 1 genes (ACY1) in Polygonatum cyrtonema Hua was identified, and associated resistance functions analyzed.
Methods Based on the transcriptome data, the family of P. cyrtonema Hua aminoacylase genes (PcACY1) was identified. Physicochemical properties, structure, conserved motifs, and evolutionary relationships of the members were analyzed. Expressions of PcACY1 in tissues/organs and under salt stress were detected by qRT-PCR.
Results The PcACY1 family had two members located in the cytoplasm, both of which were hydrophilic proteins with signal peptides and no transmembrane structure. As shown in the phylogenetic tree, they exhibited two categories of evolutionary characteristics. The predicted cis-acting elements of the plant ACY1 promoter indicated the presence of multiple hormone-, stress-, and growth/development-related functions. PcACY1 displayed significantly differentiated expressions in different tissues and organs which could be induced by salt stress.
Conclusion PcACY1 appeared to play an important role in the growth and development as well as stress resistance of P. cyrtonema .
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