Cloning and Expression under Drought of NHX6 in Hippophae rhamnoides

  Objective  NHX6 of Hippophae rhamnoides was cloned and expression under drought determined to study the stress resistance mechanism.

  Method   HrNHX6 was cloned, amplified, and sequenced for analysis by biological software. Expression of the gene under drought stress was determined.

  Result   Located on the tonoplast, HrNHX6 had no signal peptide and contained 13 transmembrane helical regions with numerous types of modification sites, such as N-glycosylation, protein kinase phosphorylation lation, myristoylation, amidation, and sugar transporter signature. The rusults of qRT-PCR analysis showed that the expression level of HrNHX6 gene was significantly different between different tissues under draught stress, and the expression level of HrNHX6 gene was significantly different between different drought stress treatment.

  Conclusion  The drought resistance of H. rhamnoides was closely related to HrNHX6 as shown by the structure and expression under drought stress of the gene. The important role indicated a significant venue for improving the draught resistance of the medicinal plant.