Active Ingredients and Antioxidative and Anti-EV71 Virus Effects of <i>Grifola frondosa</i> Ethanol Extract

XIONG Wenyu; HE Junqiang; TANG Danlin; PENG Peizhi; JIANG Xiaoqin; LIU Bin

doi:10.19303/j.issn.1008-0384.2023.04.014

Volume 38 Issue 4

Apr. 2023

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Article Navigation > Fujian Journal of Agricultural Sciences > 2023 > 38(4): 497-505

XIONG W Y, HE J Q, TANG D L, et al. Active Ingredients and Antioxidative and Anti-EV71 Virus Effects of Grifola frondosa Ethanol Extract [J]. Fujian Journal of Agricultural Sciences，2023，38（4）：497−505 doi: 10.19303/j.issn.1008-0384.2023.04.014

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XIONG W Y, HE J Q, TANG D L, et al. Active Ingredients and Antioxidative and Anti-EV71 Virus Effects of Grifola frondosa Ethanol Extract [J]. Fujian Journal of Agricultural Sciences，2023，38（4）：497−505 doi: 10.19303/j.issn.1008-0384.2023.04.014

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Active Ingredients and Antioxidative and Anti-EV71 Virus Effects of Grifola frondosa Ethanol Extract

doi: 10.19303/j.issn.1008-0384.2023.04.014

1.
College of Food Science, Fujian Agriculture and Forestry University, Fuzhou, Fujian　350002, China
2.
National Engineering Research Center of JUNCAO Technology, Fuzhou, Fujian　350002, China

Received Date: 2022-12-02
Rev Recd Date: 2022-12-22

Available Online: 2023-04-14

Publish Date: 2023-04-28

Abstract

Abstract

Objective Active ingredients and antioxidative and anti-EV71 virus activities of the Grifola frondose extracts using varied concentration of ethanol were analyzed. Method Using 55%, 70%, and 95% ethanol solutions, a ultrasound-assisted extraction was conducted to produce concoctions from G.frondosa fruiting bodies.Content of active ingredients and efficacy of the extracts obtained with varied concentrations of ethanol as an antioxidant and anti-EV71 virus agent were determined. Result The content of polyphenols at 0.042% and that of triterpenes at 1.82% were the highest in the 95% ethanol extract (GFE95) of all concoctions.The maximal polysaccharides at 33.08% and proteins at 17.45% were found in GFE55 that used 55% ethanol for the extraction.GFE95 also showed the greatest antioxidant capacity with IC₅₀ on scavenging DPPH, ABTS⁺, O₂⁻, and H₂O₂ free radicals of 0.742 7 mg·mL⁻¹, 0.741 4 mg·mL⁻¹, 0.902 6 mg·mL⁻¹, and 0.414 6 mg·mL⁻¹, respectively, and a strong antiviral effect on EV71 at the concentration of 200 μg·mL⁻¹ with the inhibition rate of 88.18%, an IC₅₀ of 194.80 μg·mL⁻¹, and a TI of 9.65.A Pearson’s correlation analysis indicated that the DPPH, ABTS⁺, and O₂⁻ free radical scavenging rates significantly correlated to the triterpenoids content with correlation coefficients of 0.992, 0.971, and 0.613, respectively (P＜0.01); the O₂⁻ and H₂O₂ free radical scavenging rates significantly correlated to the polyphenols with correlation coefficients of 0.921 and 0.997, respectively (P＜0.01); the anti-EV71 virus effect significantly correlated to the polyphenols and triterpenoids with correlation coefficients of 0.536 and 0.954, respectively (P＜0.01); and the triterpenoids and polyphenols significantly correlated to the antioxidative and antiviral activities of the extract. Conclusion The 95% ethanol extraction of G.frondosa optimally retrieved the active substances, such as polyphenols, triterpenes, and polysaccharides, in the fruiting bodies.The extract exhibited significant antioxidative and antiviral capacities, with which a valuable pharmaceutical material might be developed.
- Grifola frondosa,
- ethanol extract,
- active ingredients,
- antioxidant activity,
- antiviral activity

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