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不同品系及生长时间金线莲活性成分及挥发性物质比较分析

何辉 柳洪入 乔勇进 张怡 王春芳 陈冰洁 刘晨霞 王晓 邬松恒

何辉,柳洪入,乔勇进,等. 不同品系及生长时间金线莲活性成分及挥发性物质比较分析 [J]. 福建农业学报,2023,38(3):271−280 doi: 10.19303/j.issn.1008-0384.2023.03.003
引用本文: 何辉,柳洪入,乔勇进,等. 不同品系及生长时间金线莲活性成分及挥发性物质比较分析 [J]. 福建农业学报,2023,38(3):271−280 doi: 10.19303/j.issn.1008-0384.2023.03.003
HE H, LIU H R, QIAO Y J, et al. Active Ingredients and Volatiles in Anoectochilus roxburghii Strains at Various Growth Stages [J]. Fujian Journal of Agricultural Sciences,2023,38(3):271−280 doi: 10.19303/j.issn.1008-0384.2023.03.003
Citation: HE H, LIU H R, QIAO Y J, et al. Active Ingredients and Volatiles in Anoectochilus roxburghii Strains at Various Growth Stages [J]. Fujian Journal of Agricultural Sciences,2023,38(3):271−280 doi: 10.19303/j.issn.1008-0384.2023.03.003

不同品系及生长时间金线莲活性成分及挥发性物质比较分析

doi: 10.19303/j.issn.1008-0384.2023.03.003
基金项目: 上海市农业农村委员会项目(2020-02-08-00-15-F01455);上海市科学技术委员会项目(21DZ2292200、19DZ225160)
详细信息
    作者简介:

    何辉(1997−),男,硕士研究生,研究方向:农产品保鲜与加工(E-mail:hh18536060094@163.com

    通讯作者:

    乔勇进(1967−),男,博士,研究员,研究方向:农产品保鲜与加工(E-mail:yjqiao2002@126.com

  • 中图分类号: S567;R282.4

Active Ingredients and Volatiles in Anoectochilus roxburghii Strains at Various Growth Stages

  • 摘要:   目的  比较2个不同品系及不同生长时间金线莲主要活性成分和挥发性物质含量变化,为金线莲鉴别、筛选及评价提供理论依据。  方法  以福建小叶栽培品系土豪金以及大叶栽培品系大元宝为材料,分析评价不同生长时间金线莲中的挥发性物质并测定主要活性成分。  结果  总酚含量以种植18个月大元宝最高(265.07 mg·kg−1),总黄酮和可溶性糖均以种植12个月土豪金最高(32.68 mg·g−1、27.71 mg·kg−1),可溶性蛋白则以种植18个月的土豪金最高(600.62 mg·g−1)。通过SPME-GC-MS共检测到6种醇、8种醛、4种酮及2-戊基呋喃,其总量以种植24个月的大元宝最高,而组织培养6个月的土豪金和大元宝挥发性物质含量为栽培苗峰值的22.9%与16.7%。此外,通过气味活性值(Odor activity value,OAV)和主成分分析(Principal component analysis,PCA)发现,(反)-2-己烯醛、辛醛、壬醛是土豪金关键挥发性物质,1-辛烯-3-醇、芳樟醇和β-紫罗兰酮是大元宝关键挥发性物质。 PCA和偏最小二乘法判别分析(Partial least squares discriminant analysis,PLS-DA)表明,通过挥发性物质可以较好地区分不同品系及生长时间金线莲。  结论  不同品系及不同生长时间金线莲活性成分和挥发性物质差异显著,利用其活性成分和挥发性物质可区分不同品系及不同生长时间金线莲,这为建立适宜的质量控制体系鉴别金线莲以及筛选优质加工品种提供了依据。
  • 图  1  不同生长时间不同品系金线莲总酚(A)和总黄酮(B)含量变化

    不同大写字母表示同一品系不同生长时间之间差异显著(P<0.05),不同小写字母表示同一生长时间不同品系之间差异显著(P<0.05),下图同。

    Figure  1.  Total phenols (A) and flavonoids (B) in different strains and harvest times

    Data with different uppercase letters indicate significant differences of the same strain at different harvest times (P<0.05); those with different lowercase letters indicate significant differences between different strains at the same growth stage (P<0.05). Same for following Figs.

    图  2  不同生长时间不同品系金线莲可溶性糖(A)和可溶性蛋白(B)含量变化

    Figure  2.  Soluble sugars (A) and soluble protein (B) in different strains and harvest times

    图  3  不同品系及生长时间金线莲总挥发性物质(A)、醇类(B)、醛类(C)和酮类(D)含量变化

    Figure  3.  Total volatiles (A), alcohols (B), aldehydes (C), and ketones (D) in different strains and harvest times

    图  4  不同生长时间不同品系主要活性成分和挥发性物质PCA(左)和PLS-DA(右)分析

    A1~A3:大元宝12~24个月样品,A4:大元宝组培苗,B1~B3:土豪金12~24个月,B4:土豪金组培苗。

    Figure  4.  PCA and PLS-DA on main active ingredients and volatiles in different strains and harvest times

    A1–A3: 12–24-month-old Dayuanbao; A4: Dayuanbao tissue culture; B1–B3: 12–24-month-old Tuhaojin; B4: Tuhaojin tissue culture.

    图  5  不同生长时间不同品系挥发性物质含量

    Figure  5.  Content of volatiles in different strains and harvest times

    表  1  不同品系及采集时间挥发性物质OAV值

    Table  1.   OAV of volatiles in different strains and harvest times

    挥发性物质
    Volatile substances
    水中的阈值
    The threshold in
    water/(μg·kg−1
    品系
    Strain
    不同生长采集时间OAV值
    OAV values at different growth times
    12个月
    12 months
    18个月
    18 months
    24个月
    24 months
    组培苗
    Tissue culture
    醇类 Alcohols正己醇 1-Hexanol 5.6 土豪金 Tu Hao Jin 0.95 2.20 0.52 2.11
    大元宝 Da Yuan Bao 0.94 0.97 1.68 2.31
    1-辛烯-3-醇 1-Octen-3-ol 7 土豪金 Tu Hao Jin 9.16 47.33 31.03 1.92
    大元宝 Da Yuan Bao 18.18 65.48 541.24 2.49
    芳樟醇 Linalool 0.22 土豪金 Tu Hao Jin 8.50
    大元宝 Da Yuan Bao 14.13 17.81 23.86
    醛类 Aldehydes正己醛 Hexanal 200 土豪金 Tu Hao Jin 0.58 4.17 3.53 1.48
    大元宝 Da Yuan Bao 1.14 1.12 2.30 1.30
    庚醛 Heptanal 10 土豪金 Tu Hao Jin 1.57 1.28 2.23 2.00
    大元宝 Da Yuan Bao 1.54 1.63 2.11 1.60
    (反)-2-己烯醛 (E)-2-Hexenal 82 土豪金 Tu Hao Jin 0.29 2.11 8.95 0.77
    大元宝 Da Yuan Bao 0.68 0.56 2.00 0.95
    辛醛 Octanal 0.1 土豪金 Tu Hao Jin 380.10 278.00 980.40 193.70
    大元宝 Da Yuan Bao 295.00 278.40 599.80 295.30
    (反)-2-庚烯醛 (E)-2-Heptenal, 10 土豪金 Tu Hao Jin 0.95 1.34 1.53 1.43
    大元宝 Da Yuan Bao 1.20 1.52 2.15 2.08
    壬醛 Nonanal 3.5 土豪金 Tu Hao Jin 33.37 31.47 67.67 15.26
    大元宝 Da Yuan Bao 39.47 37.52 74.18 41.06
    苯甲醛 Benzaldehyde 3 土豪金 Tu Hao Jin 0.87 1.44 7.60
    大元宝 Da Yuan Bao 1.32 3.47 19.33
    (反)2-壬烯醛 (E)-2-Nonenal 0.07 土豪金 Tu Hao Jin 175.57 128.28 179.14 103.57
    大元宝 Da Yuan Bao 179.00 280.71 501.28 260.71
    酮类 Ketonesβ-紫罗兰酮 β-Ionone 0.007 土豪金 Tu Hao Jin 485.71 454.28 460.00
    大元宝 Da Yuan Bao 1581.42 1927.14 1221.42 1074.28
    其他 Others2-戊基呋喃 2-pentyl-Furan 4.8 土豪金 Tu Hao Jin 1.00 3.36 4.30 1.29
    大元宝 Da Yuan Bao 3.38 3.91 5.79 4.44
    —:代表未检测到。
    —:indicates no detection.
    下载: 导出CSV
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出版历程
  • 收稿日期:  2022-12-27
  • 修回日期:  2023-02-24
  • 网络出版日期:  2023-03-28
  • 刊出日期:  2023-03-28

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