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牛传染性鼻气管炎病毒糖蛋白gB和gD的研究进展

戴莎莎 王健霖 田兴苗 李继东

戴莎莎,王健霖,田兴苗,等. 牛传染性鼻气管炎病毒糖蛋白gB和gD的研究进展 [J]. 福建农业学报,2023,38(3):376−386 doi: 10.19303/j.issn.1008-0384.2023.03.015
引用本文: 戴莎莎,王健霖,田兴苗,等. 牛传染性鼻气管炎病毒糖蛋白gB和gD的研究进展 [J]. 福建农业学报,2023,38(3):376−386 doi: 10.19303/j.issn.1008-0384.2023.03.015
DAI S S, WANG J L, TIAN X M, et al. Research Progress on Glycoprotein gB and gD of Infectious Bovine Rhinotracheitis Virus [J]. Fujian Journal of Agricultural Sciences,2023,38(3):376−386 doi: 10.19303/j.issn.1008-0384.2023.03.015
Citation: DAI S S, WANG J L, TIAN X M, et al. Research Progress on Glycoprotein gB and gD of Infectious Bovine Rhinotracheitis Virus [J]. Fujian Journal of Agricultural Sciences,2023,38(3):376−386 doi: 10.19303/j.issn.1008-0384.2023.03.015

牛传染性鼻气管炎病毒糖蛋白gB和gD的研究进展

doi: 10.19303/j.issn.1008-0384.2023.03.015
基金项目: 宁夏自然科学基金项目(2022AAC03075);宁夏回族自治区重点研发计划项目(2019ZWSF3007)
详细信息
    作者简介:

    戴莎莎(1995−),女,硕士,研究方向:动物疫病诊断与防治(E-mail:1421164321@qq.com

    通讯作者:

    李继东(1976−),男,博士,副教授,研究方向:兽医微生物学与免疫学(E-mail:lijidongi@foxmail.com

  • 中图分类号: S852.65

Research Progress on Glycoprotein gB and gD of Infectious Bovine Rhinotracheitis Virus

  • 摘要: 牛传染性鼻气管炎(Infectious bovine rhinotracheitis,IBR)是牛的重要传染病,临床以呼吸道症状为主,伴有结膜炎、乳腺炎、流产等症状。其病原是牛传染性鼻气管炎病毒(Infectious bovine rhinotracheitis virus,IBRV),又称牛疱疹病毒1型(Bovine herpesvirus 1,BHV-1),共编码30~40种结构蛋白,其中11种为囊膜糖蛋白。糖蛋白在病毒吸附、侵入宿主细胞的过程中发挥重要作用。糖蛋白gB对病毒侵入宿主细胞、在细胞间扩散及复制至关重要;糖蛋白gD在病毒复制、传播和感染机制方面作用重大,具有良好的免疫原性,是诱导产生中和抗体的主要糖蛋白。对gB、gD的研究不仅可从蛋白层面解析病毒侵染机制,还能够为牛传染性鼻气管炎的临床诊断和预防提供理论依据。本文针对牛传染性鼻气管炎病毒的主要糖蛋白gB、gD的研究结果进行综述,分析其生物学功能以及在疫苗和诊断方面的应用,以期为牛传染性鼻气管炎侵染机制和防控提供参考。
  • 图  1  BHV-1 gB结构

    P17471为BHV-1 gB蛋白序列号。图片来源于InterPro蛋白数据库。

    Figure  1.  Schematic BHV-1 gB structure

    P17471: sequence number of BHV-1 gB protein; images adopted from InterPro Protein Database.

    图  2  BHV-1 gD结构

    P0CK29为BHV-1 gD蛋白序列号。图片来源于InterPro蛋白数据库。

    Figure  2.  Schematic BHV-1 gD structure

    P0CK29: the sequence number of BHV-1 gD protein. Images was a reference from the InterPro Protein Database.

    表  1  BHV-1 gB、gD蛋白功能

    Table  1.   Functions of BHV-1 gB and gD proteins

    项目 ItemBHV-1 gB功能    
    BHV-1 gB function    
    BHV-1 gD功能
    BHV-1 gD function
    靶细胞
    Target cell
    自然杀伤细胞(NK) 自然杀伤细胞(NK)、
    细胞毒性T细胞(CTL)
    主要功能
    Key function
    病毒吸附与复制;
    介导膜融合;
    肽和蛋白质的转运蛋白
    广谱溶瘤载体;
    干扰HveC介导的同源和异源α疱疹病毒的进入;
    促进免疫抑制的形成
    感染机制
    Infection mechanism
    gB和异源二聚体gH/gL组成的
    融合机制介导膜融合
    促进由gB和gH/gL组成的融合
    机制触发病毒与宿主的膜融合
    下载: 导出CSV

    表  2  疫苗优缺点

    Table  2.   Pros and cons of vaccine

    疫苗种类
    Vaccines
    载体
    Vector
    优点
    Advantages
    缺点
    Disadvantages
    DNA疫苗
    DNA vaccine
    pRSV质粒、纳米颗粒等 工艺简便,生产成本较低;DNA分子稳定,
    便于运输和保存
    产生的抗体对机体的保护不够充分
    亚单位疫苗
    Subunit vaccine
    痘病毒、腺病毒、大肠杆菌、杆状病毒、昆虫细胞、哺乳动物细胞及牛骨肉瘤细胞系D17等 接种安全,副反应少 免疫原性较低,需与佐剂合用才能产生好的免疫效果
    病毒活载体重组疫苗
    Virus live vector recombinant vaccine
    痘病毒、疱疹病毒、腺病毒、单股RNA病毒等 可以快速获得针对流行毒株的疫苗候选毒株;
    构建多联多价疫苗,减少免疫次数及改变免疫方法
    安全性较低
    下载: 导出CSV

    表  3  诊断方法

    Table  3.   Diagnostic methods

    诊断方法
    Diagnostic methods
    诊断技术
    Diagnostic technique
    BHV-1 gBBHV-1 gD
    分子生物学方法
    Molecular biology methods
    实时荧光定量PCR (qPCR)
    巢式PCR (Nested PCR)
    恒温隔绝式荧光PCR (iiPCR)
    环介导等温扩增技术(LAMP)
    聚合酶螺旋反应(PSR)






    ×

    ×
    免疫学方法
    Immunologic methods
    酶联免疫吸附试验(ELISA)
    病毒中和试验(VN )
    间接免疫荧光试验(IFA)

    ×
    ×


    免疫胶体金技术(GICA)
    “√”表示目前已有研究,“×”表示目前未见研究。
    √: existing research; ×: no known research activity at present.
    下载: 导出CSV
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  • 收稿日期:  2022-09-03
  • 修回日期:  2022-12-16
  • 网络出版日期:  2023-03-28
  • 刊出日期:  2023-03-28

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