牛传染性鼻气管炎病毒糖蛋白gB和gD的研究进展

戴莎莎; 王健霖; 田兴苗; 李继东

doi:10.19303/j.issn.1008-0384.2023.03.015

牛传染性鼻气管炎病毒糖蛋白gB和gD的研究进展

doi: 10.19303/j.issn.1008-0384.2023.03.015

宁夏大学农学院，宁夏　银川　750021

基金项目: 宁夏自然科学基金项目（2022AAC03075）；宁夏回族自治区重点研发计划项目（2019ZWSF3007）

详细信息

作者简介:
戴莎莎（1995−），女，硕士，研究方向：动物疫病诊断与防治（E-mail：1421164321@qq.com）

通讯作者:
李继东（1976−），男，博士，副教授，研究方向：兽医微生物学与免疫学（E-mail：lijidongi@foxmail.com）

中图分类号: S852.65
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- 被引次数: 0
出版历程
- 收稿日期: 2022-09-03
- 修回日期: 2022-12-16
- 网络出版日期: 2023-03-28
- 刊出日期: 2023-03-28

Research Progress on Glycoprotein gB and gD of Infectious Bovine Rhinotracheitis Virus

College of Agriculture, Ningxia University, Yinchuan, Ningxia 750021, China

摘要

摘要: 牛传染性鼻气管炎（Infectious bovine rhinotracheitis，IBR）是牛的重要传染病，临床以呼吸道症状为主，伴有结膜炎、乳腺炎、流产等症状。其病原是牛传染性鼻气管炎病毒（Infectious bovine rhinotracheitis virus，IBRV），又称牛疱疹病毒1型（Bovine herpesvirus 1，BHV-1），共编码30～40种结构蛋白，其中11种为囊膜糖蛋白。糖蛋白在病毒吸附、侵入宿主细胞的过程中发挥重要作用。糖蛋白gB对病毒侵入宿主细胞、在细胞间扩散及复制至关重要；糖蛋白gD在病毒复制、传播和感染机制方面作用重大，具有良好的免疫原性，是诱导产生中和抗体的主要糖蛋白。对gB、gD的研究不仅可从蛋白层面解析病毒侵染机制，还能够为牛传染性鼻气管炎的临床诊断和预防提供理论依据。本文针对牛传染性鼻气管炎病毒的主要糖蛋白gB、gD的研究结果进行综述，分析其生物学功能以及在疫苗和诊断方面的应用，以期为牛传染性鼻气管炎侵染机制和防控提供参考。
- 牛传染性鼻气管炎病毒 /
- gB糖蛋白 /
- gD糖蛋白 /
- 生物学功能 /
- 诊断 /
- 疫苗
Abstract: Infectious bovine rhinotracheitis is an important infectious disease of cattle. The clinical symptoms of the disease were principally respiratory ones that may be accompanied by conjunctivitis, mastitis, abortion, etc. The pathogenic virus is also known as bovine herpesvirus type 1. It encodes 30 to 40 structural proteins with 11 envelope glycoproteins, which play an important role in the process of virus adsorption and host cell invasion. Glycoprotein gB is essential for the virus to invade, spread, and replicate on host cells. Glycoprotein gD is critical in viral replication, transmission, and infection with strong immunogenicity that induces neutralizing antibodies. Studying gB and gD not only helps decipher the infection mechanism at the molecular level but also leads to new clinical diagnosis and prevention method developments on rhinotracheitis. This article summarizes recent research results on glycoprotein gB and gD concerning the biological functions and applications of these proteins in producing vaccines and generating advanced diagnosis methodologies for the infectious disease in cattle.
- Bovine infectious rhinovirus /
- glycoprotein gB /
- glycoprotein gD /
- biological function /
- diagnosis /
- vaccine

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图 1 BHV-1 gB结构

P17471为BHV-1 gB蛋白序列号。图片来源于InterPro蛋白数据库。

Figure 1. Schematic BHV-1 gB structure

P17471: sequence number of BHV-1 gB protein; images adopted from InterPro Protein Database.

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图 2 BHV-1 gD结构

P0CK29为BHV-1 gD蛋白序列号。图片来源于InterPro蛋白数据库。

Figure 2. Schematic BHV-1 gD structure

P0CK29: the sequence number of BHV-1 gD protein. Images was a reference from the InterPro Protein Database.

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表 1 BHV-1 gB、gD蛋白功能

Table 1. Functions of BHV-1 gB and gD proteins

项目 Item	BHV-1 gB功能　　　　 BHV-1 gB function	BHV-1 gD功能 BHV-1 gD function
靶细胞 Target cell	自然杀伤细胞（NK）	自然杀伤细胞（NK）、细胞毒性T细胞（CTL）
主要功能 Key function	病毒吸附与复制；介导膜融合；肽和蛋白质的转运蛋白	广谱溶瘤载体；干扰HveC介导的同源和异源α疱疹病毒的进入；促进免疫抑制的形成
感染机制 Infection mechanism	gB和异源二聚体gH/gL组成的融合机制介导膜融合	促进由gB和gH/gL组成的融合机制触发病毒与宿主的膜融合

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表 2 疫苗优缺点

Table 2. Pros and cons of vaccine

疫苗种类 Vaccines	载体 Vector	优点 Advantages	缺点 Disadvantages
DNA疫苗 DNA vaccine	pRSV质粒、纳米颗粒等	工艺简便，生产成本较低；DNA分子稳定，便于运输和保存	产生的抗体对机体的保护不够充分
亚单位疫苗 Subunit vaccine	痘病毒、腺病毒、大肠杆菌、杆状病毒、昆虫细胞、哺乳动物细胞及牛骨肉瘤细胞系D17等	接种安全，副反应少	免疫原性较低，需与佐剂合用才能产生好的免疫效果
病毒活载体重组疫苗 Virus live vector recombinant vaccine	痘病毒、疱疹病毒、腺病毒、单股RNA病毒等	可以快速获得针对流行毒株的疫苗候选毒株；构建多联多价疫苗，减少免疫次数及改变免疫方法	安全性较低

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表 3 诊断方法

Table 3. Diagnostic methods

诊断方法 Diagnostic methods	诊断技术 Diagnostic technique	BHV-1 gB	BHV-1 gD
分子生物学方法 Molecular biology methods	实时荧光定量PCR （qPCR）巢式PCR （Nested PCR）恒温隔绝式荧光PCR （iiPCR）环介导等温扩增技术（LAMP）聚合酶螺旋反应（PSR）	√ √ √ √ √	√ √ × √ ×
免疫学方法 Immunologic methods	酶联免疫吸附试验（ELISA）病毒中和试验（VN ）间接免疫荧光试验（IFA）	√ × ×	√ √ √
	免疫胶体金技术（GICA）	√	√
“√”表示目前已有研究，“×”表示目前未见研究。 √: existing research; ×: no known research activity at present.

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参考文献(77)

[1]	NAGY A, ABDALLAH F, EL DAMATY H M, et al. Genetic characterization of upper respiratory tract virome from nonvaccinated Egyptian cow-calf operations [J]. PLoS One, 2022, 17(5): e0267036. doi: 10.1371/journal.pone.0267036
[2]	HOU P L, ZHAO M, HE W Q, et al. Cellular microRNA bta-miR-2361 inhibits bovine herpesvirus 1 replication by directly targeting EGR1 gene [J]. Veterinary Microbiology, 2019, 233: 174−183. doi: 10.1016/j.vetmic.2019.05.004
[3]	WATHES D C, OGUEJIOFOR C F, THOMAS C, et al. Importance of viral disease in dairy cow fertility [J]. Engineering, 2020, 6(1): 26−33. doi: 10.1016/j.eng.2019.07.020
[4]	QUEIROZ-CASTRO V L D, DA COSTA E P, ALVES S V P, et al. Detection of bovine herpesvirus 1 in genital organs of naturally infected cows [J]. Theriogenology, 2019, 130: 125−129. doi: 10.1016/j.theriogenology.2019.03.003
[5]	TANG L K, YUAN W F, LI S T, et al. DNA damage response differentially affects BoHV-1 gene transcription in cell type-dependent manners [J]. Biomedicines, 2022, 10(9): 2282. doi: 10.3390/biomedicines10092282
[6]	OSTLER J B, JONES C. The bovine herpesvirus 1 latency-reactivation cycle, a chronic problem in the cattle industry [J]. Viruses, 2023, 15(2): 552. doi: 10.3390/v15020552
[7]	QIU W C, DING X Y, LI S T, et al. Oncolytic bovine herpesvirus 1 inhibits human lung adenocarcinoma A549 cell proliferation and tumor growth by inducing DNA damage [J]. International Journal of Molecular Sciences, 2021, 22(16): 8582. doi: 10.3390/ijms22168582
[8]	何小丽. 牛传染性鼻气管炎病毒部分gB蛋白的原核表达及应用[D]. 银川: 宁夏大学, 2018. HE X L. Prokaryotic expression and application of partial gB protein of infectious bovine rhinotracheitis virus[D]. Yinchuan: Ningxia University, 2018. (in Chinese)
[9]	李兆利, 薛飞, 朱远茂. 牛传染性鼻气管炎病毒gB蛋白研究进展 [J]. 动物医学进展, 2006, 27(4):1−4. doi: 10.3969/j.issn.1007-5038.2006.04.001 LI Z L, XUE F, ZHU Y M. Advance in Infectious bovine rhinotracheitis virus Glycoprotein B [J]. Progress in Veterinary Medicine, 2006, 27(4): 1−4.(in Chinese) doi: 10.3969/j.issn.1007-5038.2006.04.001
[10]	ROS C, BELÁK S. Characterization of the glycoprotein B gene from ruminant alphaherpesviruses [J]. Virus Genes, 2002, 24(2): 99−105. doi: 10.1023/A:1014504730475
[11]	VALLBRACHT M, LÖTZSCH H, KLUPP B G, et al. In vitro viral evolution identifies a critical residue in the alphaherpesvirus fusion glycoprotein B ectodomain that controls gH/gL-independent entry [J]. mBio, 2021, 12(3): e00557−e00521.
[12]	HUTCHINGS D L, VAN DRUNEN LITTEL-VAN DEN HURK S, BABIUK L A. Lymphocyte proliferative responses to separated bovine herpesvirus 1 proteins in immune cattle [J]. Journal of Virology, 1990, 64(10): 5114−5122. doi: 10.1128/jvi.64.10.5114-5122.1990
[13]	YUE D, CHEN Z J, YANG F L, et al. Crystal structure of bovine herpesvirus 1 glycoprotein D bound to nectin-1 reveals the basis for its low-affinity binding to the receptor [J]. Science Advances, 2020, 6(20): eaba5147. doi: 10.1126/sciadv.aba5147
[14]	HONDA T, SAKISAKA T, YAMADA T, et al. Involvement of nectins in the formation of puncta adherentia junctions and the mossy fiber trajectory in the mouse hippocampus [J]. Molecular and Cellular Neuroscience, 2006, 31(2): 315−325. doi: 10.1016/j.mcn.2005.10.002
[15]	DUMMER L A , LEITE F , HURK L. Bovine herpesvirus glycoprotein D: A review of its structural characteristics and applications in vaccinology [J]. Veterinary Research, 2014, 45(1): 111. doi: 10.1186/s13567-014-0111-x
[16]	RUDD J S, MUSARRAT F, KOUSOULAS K G. Development of a reliable bovine neuronal cell culture system and labeled recombinant bovine herpesvirus type-1 for studying virus-host cell interactions [J]. Virus Research, 2021, 293: 198255. doi: 10.1016/j.virusres.2020.198255
[17]	杨志元, 闻晓波, 冉旭华. 牛疱疹病毒Ⅰ型(BHV-1)免疫逃逸机制研究进展 [J]. 黑龙江八一农垦大学学报, 2018, 30(4):42−46. doi: 10.3969/j.issn.1002-2090.2018.04.008 YANG Z Y, WEN X B, RAN X H. Immune escape mechanism of bovine herpesvirus Ⅰ(BHV-1) [J]. Journal of Heilongjiang Bayi Agricultural University, 2018, 30(4): 42−46.(in Chinese) doi: 10.3969/j.issn.1002-2090.2018.04.008
[18]	GRABOWSKA K, WĄCHALSKA M, GRAUL M, et al. Alphaherpesvirus gB homologs are targeted to extracellular vesicles, but they differentially affect MHC class II molecules [J]. Viruses, 2020, 12(4): 429. doi: 10.3390/v12040429
[19]	KEIL G M, HÖHLE C, GIESOW K, et al. Engineering glycoprotein B of bovine herpesvirus 1 to function as transporter for secreted proteins: A new protein expression approach [J]. Journal of Virology, 2005, 79(2): 791−799. doi: 10.1128/JVI.79.2.791-799.2005
[20]	HUANG Y, BABIUK L A, VAN DRUNEN LITTEL-VAN DEN HURK S. The cell-mediated immune response induced by plasmid encoding bovine herpesvirus 1 glycoprotein B is enhanced by plasmid encoding IL-12 when delivered intramuscularly or by gene Gun, but not after intradermal injection [J]. Vaccine, 2006, 24(25): 5349−5359. doi: 10.1016/j.vaccine.2006.04.026
[21]	OSMAN N A, RÖDER A, GIESOW K, et al. Genetic fusion of peste des petits ruminants virus haemagglutinin and fusion protein domains to the amino terminal subunit of glycoprotein B of bovine herpesvirus 1 interferes with transport and function of gB for BHV-1 infectious replication [J]. Virus Research, 2018, 258: 9−18. doi: 10.1016/j.virusres.2018.09.015
[22]	NI H B, JIA X X, WANG J, et al. Mapping a highly conserved linear neutralizing epitope at the N-terminus of the gD glycoprotein of bovine herpesvirus type I using a monoclonal antibody [J]. Microbial Pathogenesis, 2020, 138: 103815. doi: 10.1016/j.micpath.2019.103815
[23]	WANG X, BI Y, RAN X H, et al. Mapping a highly conserved linear neutralizing epitope on gD glycoprotein of bovine herpesvirus type I using a monoclonal antibody [J]. The Journal of Veterinary Medical Science, 2019, 81(5): 780−786. doi: 10.1292/jvms.19-0041
[24]	翟璐, 张海威, 涂伟, 等. 牛疱疹病毒1型主要囊膜糖蛋白研究进展 [J]. 动物医学进展, 2020, 41(2):88−92. ZHAI L, ZHANG H W, TU W, et al. Progress on envelope glycoproteins of bovine herpesvirus type 1 [J]. Progress in Veterinary Medicine, 2020, 41(2): 88−92.(in Chinese)
[25]	CONNOLLY S A, WHITBECK J C, RUX A H, et al. Glycoprotein D homologs in Herpes simplex virus type 1, pseudorabies virus, and bovine Herpes virus type 1 bind directly to human HveC (nectin-1) with different affinities [J]. Virology, 2001, 280(1): 7−18. doi: 10.1006/viro.2000.0747
[26]	CUDDINGTON B P, MOSSMAN K L. Oncolytic bovine herpesvirus type 1 as a broad spectrum cancer therapeutic [J]. Current Opinion in Virology, 2015, 13: 11−16. doi: 10.1016/j.coviro.2015.03.010
[27]	李河林. IBRV XA株的分离鉴定及其gD蛋白的原核表达[D]. 杨凌: 西北农林科技大学, 2010. LI H L. Isolation and identification of IBRV and prokaryotic expression its glycoprotein D[D]. Yangling: Northwest A & F University, 2010. (in Chinese)
[28]	LIU Y, ZHANG Q, ZOU M, et al. Cell entry of Bovine herpesvirus-1 through clathrin- and caveolin-mediated endocytosis requires activation of PI3K-Akt-NF-κB and Ras-p38 MAPK pathways as well as the interaction of BoHV-1 gD with cellular receptor nectin-1 [J]. Veterinary Microbiology, 2023, 279: 109672. doi: 10.1016/j.vetmic.2023.109672
[29]	HUANG Y , BABIUK L A , HURK L . Immunization with a bovine herpesvirus 1 glycoprotein B DNA vaccine induces cytotoxic T-lymphocyte responses in mice and cattle[J]. The Journal of General Virology, 2005, 86(Pt 4): 887-898.
[30]	TOUSSAINT J F, COEN L, LETELLIER C, et al. Genetic immunisation of cattle against bovine herpesvirus 1: Glycoprotein gD confers higher protection than glycoprotein gC or tegument protein VP8 [J]. Veterinary Research, 2005, 36(4): 529−544. doi: 10.1051/vetres:2005015
[31]	LEWIS P J, COX G J M, VAN DRUNEN LITTEL-VAN DEN HURK S, et al. Polynucleotide vaccines in animals: Enhancing and modulating responses [J]. Vaccine, 1997, 15(8): 861−864. doi: 10.1016/S0264-410X(96)00279-4
[32]	LIU X B, YU G W, GAO X Y, et al. Intranasal delivery of plasmids expressing bovine herpesvirus 1 gB/gC/gD proteins by polyethyleneimine magnetic beads activates long-term immune responses in mice [J]. Virology Journal, 2021, 18(1): 60. doi: 10.1186/s12985-021-01536-w
[33]	CASELLI E, BONI M, DI LUCA D, et al. A combined bovine herpesvirus 1 gB-gD DNA vaccine induces immune response in mice [J]. Comparative Immunology, Microbiology and Infectious Diseases, 2005, 28(2): 155−166. doi: 10.1016/j.cimid.2004.10.001
[34]	DI GIACOMO S, QUATTROCCHI V, ZAMORANO P. Use of adjuvants to enhance the immune response induced by a DNA vaccine against bovine herpesvirus-1 [J]. Viral Immunology, 2015, 28(6): 343−346. doi: 10.1089/vim.2014.0113
[35]	ZAMORANO P, TABOGA O, DOMı́NGUEZ M, et al. BHV-1 DNA vaccination: Effect of the adjuvant RN-205 on the modulation of the immune response in mice [J]. Vaccine, 2002, 20(21/22): 2656−2664.
[36]	KORNUTA C A, LANGELLOTTI C A, BIDART J E, et al. A plasmid encoding the extracellular domain of CD40 ligand and Montanide™ GEL01 as adjuvants enhance the immunogenicity and the protection induced by a DNA vaccine against BoHV-1 [J]. Vaccine, 2021, 39(6): 1007−1017. doi: 10.1016/j.vaccine.2020.11.071
[37]	NANDI S, KUMAR M, MANOHAR M, et al. Bovine herpes virus infections in cattle [J]. Animal Health Research Reviews, 2009, 10(1): 85−98. doi: 10.1017/S1466252309990028
[38]	ABDELMAGID O Y, MANSOUR M M, MINOCHA H C, et al. Evaluation of baculovirus-expressed bovine herpesvirus-1 (BHV-1) glycoproteins for detection and analysis of BHV-1-specific antibody responses [J]. Veterinary Microbiology, 1998, 61(4): 249−259. doi: 10.1016/S0378-1135(98)00188-6
[39]	PERALTA A, MOLINARI P, CONTE-GRAND D, et al. A chimeric baculovirus displaying bovine herpesvirus-1 (BHV-1) glycoprotein D on its surface and their immunological properties [J]. Applied Microbiology and Biotechnology, 2007, 75(2): 407−414. doi: 10.1007/s00253-006-0825-4
[40]	OLIVEIRA S C, HARMS J S, ROSINHA G M S, et al. Biolistic-mediated gene transfer using the bovine herpesvirus-1 glycoprotein D is an effective delivery system to induce neutralizing antibodies in its natural host [J]. Journal of Immunological Methods, 2000, 245(1/2): 109−118.
[41]	HOU L N, WANG F X, WANG Y X, et al. Subunit vaccine based on glycoprotein B protects pattern animal Guinea pigs from tissue damage caused by infectious bovine rhinotracheitis virus [J]. Virus Research, 2022, 320: 198899. doi: 10.1016/j.virusres.2022.198899
[42]	谢青梅, 封柯宇, 沈勇. 动物病毒重组活载体疫苗研究进展 [J]. 华南农业大学学报, 2019, 40(5):102−110. doi: 10.7671/j.issn.1001-411X.201905060 XIE Q M, FENG K Y, SHEN Y. Advances in recombinant live vector vaccines for animal viruses [J]. Journal of South China Agricultural University, 2019, 40(5): 102−110.(in Chinese) doi: 10.7671/j.issn.1001-411X.201905060
[43]	ERTL H C. Viral vectors as vaccine carriers [J]. Current Opinion in Virology, 2016, 21: 1−8. doi: 10.1016/j.coviro.2016.06.001
[44]	KHATTAR S K, COLLINS P L, SAMAL S K. Immunization of cattle with recombinant Newcastle disease virus expressing bovine herpesvirus-1 (BHV-1) glycoprotein D induces mucosal and serum antibody responses and provides partial protection against BHV-1 [J]. Vaccine, 2010, 28(18): 3159−3170. doi: 10.1016/j.vaccine.2010.02.051
[45]	SUBBIAH M, YAN Y Q, ROCKEMANN D, et al. Experimental infection of calves with Newcastle disease virus induces systemic and mucosal antibody responses [J]. Archives of Virology, 2008, 153(6): 1197−1200. doi: 10.1007/s00705-008-0099-5
[46]	GOGEV S, VANDERHEIJDEN N, LEMAIRE M, et al. Induction of protective immunity to bovine herpesvirus type 1 in cattle by intranasal administration of replication-defective human adenovirus type 5 expressing glycoprotein gC or gD [J]. Vaccine, 2002, 20(9/10): 1451−1465.
[47]	PÉREZ FILGUEIRA D M, ZAMORANO P I, DOMı́NGUEZ M G, et al. Bovine herpes virus gD protein produced in plants using a recombinant tobacco mosaic virus (TMV) vector possesses authentic antigenicity [J]. Vaccine, 2003, 21(27/28/29/30): 4201−4209.
[48]	LIU C Y, GUO H, ZHAO H Z, et al. Recombinant bovine herpesvirus type I expressing the bovine viral diarrhea virus E2 protein could effectively prevent infection by two viruses [J]. Viruses, 2022, 14(8): 1618. doi: 10.3390/v14081618
[49]	BILGE-DAGALP S, FARZANI T A, DOGAN F, et al. Development of a BoHV-4 viral vector expressing tgD of BoHV-1 and evaluation of its immunogenicity in mouse model [J]. Brazilian Journal of Microbiology, 2021, 52(3): 1119−1133. doi: 10.1007/s42770-021-00525-z
[50]	SHRINGI S, O'TOOLE D, COLE E, et al. OvHV-2 glycoprotein B delivered by a recombinant BoHV-4 is immunogenic and induces partial protection against sheep-associated malignant catarrhal fever in a rabbit model [J]. Vaccines, 2021, 9(2): 90. doi: 10.3390/vaccines9020090
[51]	丁国伟, 李琛, 李玉安, 等. 牛传染性鼻气管炎病毒gD蛋白的原核表达及其免疫原性 [J]. 湖南农业大学学报(自然科学版), 2018, 44(1):77−81. DING G W, LI C, LI Y A, et al. Expression and immunogenicity of protein gD from virus in infectious rhinotracheitis of bovine [J]. Journal of Hunan Agricultural University (Natural Sciences), 2018, 44(1): 77−81.(in Chinese)
[52]	乔波, 陈楠楠, 赵静虎, 等. 牛传染性鼻气管炎病毒TaqMan-MGB荧光定量PCR方法的建立 [J]. 中国预防兽医学报, 2015, 37(4):282−285. doi: 10.3969/j.issn.1008-0589.2015.04.11 QIAO B, CHEN N N, ZHAO J H, et al. Development of TaqMan-MGB probe real-time PCR for detection of infectious bovine rhinotracheitis virus [J]. Chinese Journal of Preventive Veterinary Medicine, 2015, 37(4): 282−285.(in Chinese) doi: 10.3969/j.issn.1008-0589.2015.04.11
[53]	MARIN M S, QUINTANA S, LEUNDA M R, et al. A new method for simultaneous detection and discrimination of Bovine herpesvirus types 1 (BoHV-1) and 5 (BoHV-5) using real time PCR with high resolution melting (HRM) analysis [J]. Journal of Virological Methods, 2016, 227: 14−22. doi: 10.1016/j.jviromet.2015.10.005
[54]	任亚初, 楚会萌, 程凯慧, 等. 牛传染性鼻气管炎病毒SYBR GreenⅠ荧光定量PCR检测方法的建立及应用 [J]. 中国预防兽医学报, 2019, 41(10):1032−1036. REN Y C, CHU H M, CHENG K H, et al. Establishment and application of the SYBY Green I real-time PCR assay for detection of the infectious bovine rhinotracheitis virus [J]. Chinese Journal of Preventive Veterinary Medicine, 2019, 41(10): 1032−1036.(in Chinese)
[55]	王倩颖, 杨森, 刘可欣, 等. 牛传染性鼻气管炎病毒SYBR Green I实时荧光定量PCR检测方法的建立与初步应用[J/OL]. 中国动物传染病学报: 1-8 [2023-03-24]. https://doi.org/10.19958/j.cnki.cn31-2031/s.20211103.001. WANG Q Y, YANG S, LIU K X, et al. Establishment and preliminary application of SYBR Green I real-time fluorescent quantitative PCR for detection of infectious bovine rhinotracheitis virus[J/OL]. Chinese Journal of Animal Infectious Diseases: 1-8 [2023-03-24]. https://doi.org/10.19958/j.cnki.cn31-2031/s.20211103.001. (in Chinese)
[56]	任强林. 牛传染性鼻气管炎病毒TaqMan-gB荧光定量PCR检测方法的建立及初步应用[D]. 乌鲁木齐: 新疆农业大学, 2021. REN Q L. Establishment and preliminary application of real-time PCR for detection of infectious bovine infectious rhinotracheitis virus[D]. Urumqi: Xinjiang Agricultural University, 2021. (in Chinese)
[57]	EL-KHOLY A A. Molecular and immunological detection of bovine herpesvirus-1 in clinical specimens [J]. The Egyptian Journal of Immunology, 2005, 12(2): 125−136.
[58]	邓碧华. 牛传染性鼻气管炎病毒套式PCR和荧光PCR检测方法的建立[D]. 南京: 南京农业大学, 2006. DENG B H. Establishment of nested PCR and real-time PCR methods to detect infectious bovine rhinotracheitis virus[D]. Nanjing: Nanjing Agricultural University, 2006. (in Chinese)
[59]	HIDAYATI D N, UNTARI T, WIBOWO M H, et al. Cloning and sequencing gB, gD, and gM genes to perform the genetic variability of bovine herpesvirus-1 from Indonesia [J]. Veterinary World, 2018, 11(9): 1255−1261. doi: 10.14202/vetworld.2018.1255-1261
[60]	DU T, LIN J H, ZHAO J H, et al. Development and evaluation of an iiPCR assay for Salmonella and Shigella detection on a field-deployable PCR system [J]. The Canadian Journal of Infectious Diseases & Medical Microbiology, 2020, 2020: 9373984.
[61]	张颖慧. 肉牛呼吸道疾病综合征的病原检测和IBRV恒温隔绝式荧光PCR方法的建立与应用[D]. 成都: 西南民族大学, 2019. ZHANG Y H. Detection of pathogens from beef cattle with bovine respiratory disease complex and establishment and application of an insulated isothermal PCR for on-site detecting IBRV[D]. Chengdu: Southwest University for Nationalities, 2019. (in Chinese)
[62]	SOCHA W, ROLA J, URBAN-CHMIEL R, et al. Application of loop-mediated isothermal amplification (LAMP) assays for the detection of bovine herpesvirus 1 [J]. Polish Journal of Veterinary Sciences, 2017, 20(3): 619−622. doi: 10.1515/pjvs-2017-0078
[63]	DONG S J, FENG M, YU R S, et al. Establishment and application of visual LAMP detection method of infectious bovine rhinotracheitis virus [J]. Chinese Journal of Biotechnology, 2018, 34(10): 1587−1595.
[64]	FAN Q, XIE Z X, XIE Z Q, et al. Development of duplex fluorescence-based loop-mediated isothermal amplification assay for detection of Mycoplasma bovis and bovine herpes virus 1 [J]. Journal of Virological Methods, 2018, 261: 132−138. doi: 10.1016/j.jviromet.2018.08.014
[65]	MALLA J A, CHAKRAVARTI S, GUPTA V, et al. Novel Polymerase Spiral Reaction (PSR) for rapid visual detection of Bovine Herpesvirus 1 genomic DNA from aborted bovine fetus and semen [J]. Gene, 2018, 644: 107−112. doi: 10.1016/j.gene.2017.11.004
[66]	马思续, 崔春晓, 张留君, 等. 不同包被抗原检测PRRS抗体间接ELISA方法的建立 [J]. 中国兽医学报, 2018, 38(6):1082−1087. MA S X, CUI C X, ZHANG L J, et al. Establishment of the indirect ELISA method for detecting PRRS antibody using different antigens [J]. Chinese Journal of Veterinary Science, 2018, 38(6): 1082−1087.(in Chinese)
[67]	费玮彦. 牛传染性鼻气管炎病毒SH7株gB、gD基因的原核表达及ELISA方法的建立[D]. 合肥: 安徽农业大学, 2015. FEI W Y. Prokaryotic expression of infectious bovine rhinotracheitis virus SH7 isolate glycoprotein B and glycoprotein D and establishment of ELISA methods[D]. Hefei: Anhui Agricultural University, 2015. (in Chinese)
[68]	周跃辉. 牛传染性鼻气管炎病毒糖蛋白gD单抗制备及其抗原表位鉴定与双抗夹心ELISA的建立[D]. 北京: 中国农业科学院, 2015. ZHOU Y H. Identification of an antigen epitope on the glycoprotein D with monoclonal antibody and establishment of A double antibody sandwich ELISA for detection for infectious bovine rhinotracheitis virus[D]. Beijing: Chinese Academy of Agricultural Sciences, 2015. (in Chinese)
[69]	魏鑫, 张建华, 郭婷, 等. IBRV gD蛋白的原核表达及其间接ELISA检测方法的建立 [J]. 中国兽医学报, 2019, 39(11):2129−2134. WEI X, ZHANG J H, GUO T, et al. Prokaryotic expression for gD protein of infectious bovine rhinotracheitis virus and establishment of indirect ELISA-linked testing method [J]. Chinese Journal of Veterinary Science, 2019, 39(11): 2129−2134.(in Chinese)
[70]	史喜绢, 杨博, 张婷, 等. 牛传染性鼻气管炎病毒gB ELISA抗体与中和抗体相关性分析[J/OL]. 中国动物传染病学报: 1-8 [2023-03-24]. https://doi.org/10.19958/j.cnki.cn31-2031/s.20210817.012. SHI X J, YANG B, ZHANG T, et al. Correlation analysis between gB ELISA antibody and neutralizing antibody of infectious bovine rhinotracheitis virus[J/OL]. Chinese Journal of Animal Infectious Diseases, : 1-8[2023-03-24]. https://doi.org/10.19958/j.cnki.cn31-2031/s.20210817.012. (in Chinese)
[71]	LIU W X, HONG J B, DUAN J L, et al. A neutralizing monoclonal antibody–based blocking ELISA to detect bovine herpesvirus 1 and vaccination efficacy [J]. Applied Microbiology and Biotechnology, 2023, 107(1): 379−390. doi: 10.1007/s00253-022-12308-z
[72]	吴靖. 抗牛传染性鼻气管炎病毒囊膜gD糖蛋白单链抗体的制备与特性鉴定[D]. 南昌: 江西农业大学, 2017. WU J. Development and characterization of single chain antibody against gD glycoprotein of bovine infectious rhinotracheitis virus[D]. Nanchang: Jiangxi Agricultural University, 2017. (in Chinese)
[73]	毕莹, 闻晓波, 倪宏波. 牛传染性鼻气管炎病毒gD蛋白单克隆抗体的制备及鉴定 [J]. 中国生物制品学杂志, 2017, 30(10):1050−1054. BI Y, WEN X B, NI H B. Preparation and identification of monoclonal antibody against gD protein of bovine infectious rhinotracheitis virus [J]. Chinese Journal of Biologicals, 2017, 30(10): 1050−1054.(in Chinese)
[74]	贾晓雪, 赵微, 倪宏波. 抗牛传染性鼻气管炎病毒gD蛋白单克隆抗体的制备及鉴定 [J]. 中国生物制品学杂志, 2019, 32(7):777−780,785. JIA X X, ZHAO W, NI H B. Preparation and identification of monoclonal antibody against gD protein of bovine infectious rhinotracheitis virus [J]. Chinese Journal of Biologicals, 2019, 32(7): 777−780,785.(in Chinese)
[75]	杨飞, 黄小洁, 刘丹, 等. 牛传染性鼻气管炎病毒gD蛋白单克隆抗体的制备与鉴定 [J]. 动物医学进展, 2020, 41(3):7−11. doi: 10.3969/j.issn.1007-5038.2020.03.002 YANG F, HUANG X J, LIU D, et al. Preparation and identification of monoclonal antibodies against gD protein of infectious bovine rhinotracheitis virus [J]. Progress in Veterinary Medicine, 2020, 41(3): 7−11.(in Chinese) doi: 10.3969/j.issn.1007-5038.2020.03.002
[76]	梅力, 李永清, 宋彦军, 等. 牛传染性鼻气管炎病毒抗体胶体金检测试纸条的制备 [J]. 中国兽医杂志, 2019, 55(1):39−43,5. MEI L, LI Y Q, SONG Y J, et al. Development of acolloidal gold test strip for detection antibody of Infectious Bovine Rhinotracheitis Virus [J]. Chinese Journal of Veterinary Medicine, 2019, 55(1): 39−43,5.(in Chinese)
[77]	EISENBERG R J, ATANASIU D, CAIRNS T M, et al. Herpes virus fusion and entry: A story with many characters [J]. Viruses, 2012, 4(5): 800−832. doi: 10.3390/v4050800