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中国沙棘HrNHX6基因克隆及其在干旱胁迫下的表达分析

罗程月 马玉花 王晨兆 张丹 董佳伟 冶贵生

罗程月,马玉花,王晨兆,等. 中国沙棘HrNHX6基因克隆及其在干旱胁迫下的表达分析 [J]. 福建农业学报,2023,38(7):809−816 doi: 10.19303/j.issn.1008-0384.2023.07.006
引用本文: 罗程月,马玉花,王晨兆,等. 中国沙棘HrNHX6基因克隆及其在干旱胁迫下的表达分析 [J]. 福建农业学报,2023,38(7):809−816 doi: 10.19303/j.issn.1008-0384.2023.07.006
LUO C Y, MA Y H, WANG C Z, et al. Cloning and Expression under Drought of NHX6 in Hippophae rhamnoides [J]. Fujian Journal of Agricultural Sciences,2023,38(7):809−816 doi: 10.19303/j.issn.1008-0384.2023.07.006
Citation: LUO C Y, MA Y H, WANG C Z, et al. Cloning and Expression under Drought of NHX6 in Hippophae rhamnoides [J]. Fujian Journal of Agricultural Sciences,2023,38(7):809−816 doi: 10.19303/j.issn.1008-0384.2023.07.006

中国沙棘HrNHX6基因克隆及其在干旱胁迫下的表达分析

doi: 10.19303/j.issn.1008-0384.2023.07.006
基金项目: 国家自然科学基金项目(31660071);青海省科技厅自然科学基金项目(2017-ZJ-734);青海省高端创新人才“千人计划”项目(2021年)
详细信息
    作者简介:

    罗程月(1994 —),女,硕士研究生,主要从事林木遗传育种研究,E-mail:1767455028@qq.com

    通讯作者:

    马玉花(1978 —),女,博士,教授,主要从事森林培育理论与技术、植物资源开发利用研究,E-mail:qhxnmyh@163.com

  • 中图分类号: S793.6

Cloning and Expression under Drought of NHX6 in Hippophae rhamnoides

  • 摘要:   目的  克隆中国沙棘HrNHX6基因,分析其在干旱胁迫下的表达,以期为HrNHX6基因的抗旱功能研究提供参考依据。  方法  对中国沙棘HrNHX6基因进行扩增,并利用生物软件分析HrNHX6基因序列,预测其蛋白结构,在此基础上对干旱胁迫下HrNHX6基因的表达进行分析。  结果  获得的HrNHX6基因属于NHX家族NHX6基因,其编码蛋白质定位于液泡膜,无信号肽,含有13个跨膜螺旋区,具有N-糖基化位点、蛋白激酶磷酸化位点、豆蔻酰化位点、酰胺化位点、糖转运蛋白signature 位点等多种类型的修饰位点。qRT-PCR分析结果表明,干旱胁迫下不同组织之间HrNHX6基因的表达水平具显著差异,而不同干旱程度胁迫处理之间HrNHX6基因的表达水平呈极显著差异。  结论  HrNHX6蛋白的结构特性及HrNHX6基因在干旱胁迫下的表达模式表明其与中国沙棘抗旱关系密切且受干旱胁迫诱导。
  • 图  1  HrNHX6基因PCR产物电泳结果

    Figure  1.  Amplified HrNHX6

    图  2  NHX6蛋白质系统发育树和保守基序

    a:HrNHX6的系统发育树;b:HrNHX6的保守基序。

    Figure  2.  Phylogenetic tree and conserved motifs of NHX6 protein

    a: Phylogenetic tree of HrNHX; b: Conserved motifs of HrNHX6.

    图  3  HrNHX6与其他植物NHX6蛋白序列比对分析

    A:氨氯吡嗪脒结合位点,B:Na+/H+交换泵。黑色背景表示相同氨基酸,红色、蓝色阴影分别表示75%、50%以上的保守性。

    Figure  3.  Alignment on amino acid residues of HrNHX6 and genes of other plants

    A: Binding sites for ammonia chloride pyrazine microphones; B: Na+/H+ exchange pump; black background represents same amino acid; red and blue shadows, more than 75% and 50% conservatism, respectively.

    图  4  HrNHX6蛋白二级结构

    Figure  4.  Secondary structure of HrNHX6 protein

    图  5  沙棘HrNHX6蛋白跨膜结构域

    Figure  5.  Transmembrane structures of HrNHX6 protein

    图  6  干旱胁迫下HrNHX6基因表达模式

    不同大写字母表示不同胁迫处理时间下差异极显著(P<0.01),不同小写字母表示不同组织之间差异显著(P<0.05)。

    Figure  6.  Expression of HrNHX6 under drought stress

    Different capital letters indicate that the difference is extremely significant under different stress treatment time at P<0.01; those with different lowercase letters, significant difference between different tissues at P<0.05.

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出版历程
  • 收稿日期:  2022-10-23
  • 修回日期:  2023-04-07
  • 网络出版日期:  2023-08-16
  • 刊出日期:  2023-07-28

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