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不结球白菜与苗用大白菜远缘杂交农艺性状的遗传分析

邵贵荣 曹萍 朱彬 林晓 方勇 王树彬 马馨馨 钟凤林

邵贵荣,曹萍,朱彬,等. 不结球白菜与苗用大白菜远缘杂交农艺性状的遗传分析 [J]. 福建农业学报,2023,38(9):1047−1053 doi: 10.19303/j.issn.1008-0384.2023.09.006
引用本文: 邵贵荣,曹萍,朱彬,等. 不结球白菜与苗用大白菜远缘杂交农艺性状的遗传分析 [J]. 福建农业学报,2023,38(9):1047−1053 doi: 10.19303/j.issn.1008-0384.2023.09.006
SHAO G R, CAO P, ZHU B, et al. Agronomic Inheritance of Distant Hybrids of Non-heading and Leafy Chinese Cabbages [J]. Fujian Journal of Agricultural Sciences,2023,38(9):1047−1053 doi: 10.19303/j.issn.1008-0384.2023.09.006
Citation: SHAO G R, CAO P, ZHU B, et al. Agronomic Inheritance of Distant Hybrids of Non-heading and Leafy Chinese Cabbages [J]. Fujian Journal of Agricultural Sciences,2023,38(9):1047−1053 doi: 10.19303/j.issn.1008-0384.2023.09.006

不结球白菜与苗用大白菜远缘杂交农艺性状的遗传分析

doi: 10.19303/j.issn.1008-0384.2023.09.006
基金项目: 国家青梗菜良种重大科研联合攻关项目(111821301354052283);特色蔬菜育种攻关与产业化开发项目(zycxny2021009-1);现代蔬菜产业技术体系项目(闽财指〔2019〕897号);福建省科技计划项目(2021S0006);福州市科技计划项目(2022-P-003)
详细信息
    作者简介:

    邵贵荣(1971 —),男,副研究员,主要从事十字花科蔬菜遗传育种研究,E-mail:506747948@qq.com

    通讯作者:

    钟凤林(1977—),男,教授,博(硕)士研究生导师,主要从事设施植物遗传资源与生物技术、蔬菜生理生化和蔬菜资源与产业化研究,E-mail:zhong591@fafu.edu.cn

  • 中图分类号: S634

Agronomic Inheritance of Distant Hybrids of Non-heading and Leafy Chinese Cabbages

  • 摘要:   目的  远缘杂交是提高不结球白菜产量、品质、抗逆性和实现种质创新的重要手段之一,南方夏季高温影响不结球白菜周年生产和市场供应,远缘杂交可以丰富不结球白菜的品种供应,拓宽其遗传背景。  方法  以3个不结球白菜亲本和3个苗用大白菜亲本分别进行远缘杂交,测定亲本和F1代的农艺性状,进行遗传倾向和相关性分析。  结果  在远缘杂交中单株重的遗传力最高,对后代的遗传效果最稳定;叶色值的变异系数最高,人工选择潜力最大;株高、株幅、叶长、叶柄长、叶柄宽、单株重的超中优势和超亲优势较高,在后代中具有杂种优势。  结论  筛选出D2和Q2的亲本组合可以用来选育农艺性状突出的不结球白菜品种,D3和Q3的亲本组合可以用来选育丰产的不结球白菜品种。
  • 图  1  远缘杂交亲本及组合F1代

    Figure  1.  Parents and F1 hybrids of distant hybridization

    表  1  亲本和F1代农艺性状比较

    Table  1.   Agronomic characteristics of parents and F1 hybrids

    指标
    Indicator
    株高
    Plant height/cm
    株幅
    Plant spread/cm
    叶色值
    Leaf color index
    叶宽
    Leaf width/cm
    叶长
    Leaf length/cm
    叶柄长
    Petiole length/cm
    叶柄宽
    Petiole width/cm
    单株重
    Weight per plant/g
    D120.56±1.46 e33.76±3.98 b12.14±1.22 c15.56±1.62 d24.56±1.42 e5.00±1.10 d2.74±0.34 bc107.04±2.14 e
    Q115.32±1.09 f25.38±1.36 c3.41±0.65 d17.92±0.67 bcd9.68±1.85 f5.82±0.36 cd2.14±0.17 d63.56±0.94 g
    D227.00±1.51 d34.24±3.46 b23.44±2.23 b17.28±0.94 bcd27.52±2.33 d5.32±0.60 d2.86±0.40bc192.96±4.23 c
    Q214.84±0.78 f24.84±2.43 c5.43±0.36 d18.78±1.69 abc8.98±1.05 f4.54±0.29 d1.46±0.30e23.94±1.30 h
    D341.52±1.55 a40.88±4.43 a42.25±4.76 a17.06±0.90 bcd33.84±0.93 c12.00±1.07 a2.38±0.33
    cd
    126.34±2.20 d
    Q317.10±1.61 f26.64±2.44 c13.96±3.24 c20.56±1.67 a11.44±0.82 f6.14±0.42 cd2.12±0.15 d68.36±1.30 f
    QD-125.96±1.18 d40.18±3.09 a6.08±0.86 d16.12±1.30 cd28.62±3.28 d7.28±0.68 c2.76±0.37 bc194.72±2.51 c
    QD-229.60±2.41 c40.36±0.70 a4.84±0.59 d19.32±1.27 ab37.24±1.71 b8.74±0.29 b3.06±0.17 ab226.64±1.69 a
    QD-337.78±1.97 b40.06±3.95 a5.74±0.64 d17.94±2.00 bcd42.42±3.11 a11.28±2.36 a3.36±0.43 a206.10±1.83 b
    最大值
    Max
    43.8045.3045.3022.6044.7012.603.90229.10
    最小值
    Min
    15.1021.202.3313.307.604.101.1062.30
    F131.1140.205.5517.7936.099.103.06209.15
    标准差
    Standard deviation
    9.277.1112.231.9611.952.770.6270.02
    变异系数
    Coefficient of variation/%
    29.8117.68220.2011.0133.1030.4920.2033.48
    遗传力
    Heritability/%
    136.92129.8633.1199.63186.66140.65134.01215.55
    同列数据后不同小写字母表示各材料差异显著(P<0.05)。下同。
    Values marked with different lowercase letters in the column are significant difference materials (P<0.05). The same below.
    下载: 导出CSV

    表  2  F1代性状的杂种优势

    Table  2.   Heterosis of F1 hybrids

    品种 VarietyQD-1QD-2QD-3
    株高 Plant height/cm H1 35.33 30.56 19.34
    H2 44.70 41.49 28.90
    株幅 Plant spread/cm H1 28.29 27.67 13.91
    H2 35.88 36.63 18.66
    叶色值 Leaf color index H1 −12.72 −37.88 −49.37
    H2 −21.82 −66.47 −79.56
    叶宽 Leaf width/cm H1 −3.35 6.00 −3.85
    H2 −3.70 7.15 −4.63
    叶长 Leaf length/cm H1 43.56 63.72 56.68
    H2 67.17 104.05 87.37
    叶柄长 Petiole length/cm H1 29.22 64.58 16.25
    H2 34.57 77.28 24.37
    叶柄宽 Petiole width/cm H1 10.32 27.27 38.28
    H2 13.11 41.67 49.33
    单株重 Weight per plant/g H1 98.93 59.75 84.04
    H2 128.28 108.98 111.71
    H1:超亲优势(%);H2:超中优势(%)。
    H1 refers to over high parent heterosis, H2 refers to over the mean of parent heterosis.
    下载: 导出CSV

    表  3  亲本和F1代光合特性比较

    Table  3.   Photosynthetic properties of parents and F1 hybrids

    指标
    Indicator
    蒸腾速率
    Transpiration
    rate/
    (mmol·m−2·s−1
    净光合速率
    Net photosyntheti
    rate/
    (µmol·m−2·s−1
    胞间CO2浓度
    Intercellular CO2
    concentration/
    (µmol·mol−1
    气孔导度
    Stomatal
    conductance/
    (mmol·m−2·s−1
    实际光化学电子效率
    The actual
    photochemical
    efficiency
    最大光化学效率
    Maximum
    photochemical
    efficiency
    D13.824±2.98 abc4.668±4.88 ab342.114±19.25 a0.118±0.11 ab0.108±0.06 ab0.610±0.24 a
    Q10.476±0.05 c2.068±1.03 b299.204±21.26 bc0.034±0.02 b0.038±0.01 b0.630±0.21 a
    D24.062±0.82 ab8.056±2.75 a301.484±29.17 bc0.146±0.04 ab0.134±0.06 a0.620±0.22 a
    Q22.590±0.59 bc8.640±1.83 a291.510±14.65 c0.142±0.04 ab0.118±0.04 a0.616±0.03 a
    D30.928±0.12 bc1.872±0.89 b337.138±12.41 ab0.048±0.03 b0.034±0.03 b0.458±0.11 a
    Q31.748±0.09 bc5.970±0.75 ab287.852±20.37 c0.094±0.02 ab0.082±0.02 ab0.620±0.01 a
    QD-16.124±4.14 a8.036±5.05 a307.156±19.45 abc0.206±0.17 a0.114±0.05 a0.742±0.23 a
    QD-22.444±0.67 bc8.554±1.92 a300.330±12.42 bc0.138±0.07 ab0.120±0.03 a0.676±0.18 a
    QD-31.020±0.18 bc1.138±0.63 b332.106±32.50 ab0.026±0.01 b0.034±0.03 b0.482±0.01 a
    下载: 导出CSV

    表  4  亲本和F1代指标之间的相关性

    Table  4.   Correlation among indicators of parents and F1 hybrids

    相关性
    Correlation
    株高
    Plant height
    株幅
    Plant spread
    叶色值
    Leaf color index
    叶宽
    Leaf width
    叶长
    Leaf length
    叶柄长
    Petiole length
    叶柄宽
    Petiole width
    单株重
    Weight per plant
    蒸腾速率
    Transpiration rate
    −0.067 −0.115 0.269 −0.138 −0.105 −0.314* −0.055 −0.080
    净光合速率
    Net photosynthetic rate
    −0.019 −0.165 0.451** 0.186 −0.188 −0.111 −0.297* −0.243
    胞间CO2浓度
    Intercellular CO2 concentration
    −0.020 0.185 −0.206 −0.355* 0.178 −0.145 0.270 0.153
    气孔导度
    Stomatal conductance
    −0.023 −0.105 0.357* −0.026 −0.120 −0.203 −0.117 −0.115
    实际光化学电子效率
    The actual photochemical efficiency
    −0.100 −0.201 0.367* 0.127 −0.228 −0.204 −0.286 −0.315*
    最大光化学效率
    Maximum photochemical efficiency
    −0.172 −0.162 0.168 0.207 −0.186 −0.180 −0.273 −0.154
    株高
    Plant height
    1
    株幅
    Plant spread
    0.801** 1
    叶色值
    Leaf color index
    0.530** 0.302* 1
    叶宽
    Leaf width
    −0.170 −0.211 −0.125 1
    叶长
    Leaf length
    0.874** 0.878** 0.227 −0.212 1
    叶柄长
    Petiole length
    0.846** 0.640** 0.377* −0.059 0.703** 1
    叶柄宽
    Petiole width
    0.563** 0.675** −0.027 −0.223 0.755** 0.429** 1
    单株重
    Weight per plant
    0.671** 0.794** 0.022 −0.176 0.873** 0.475** 0.814** 1
    **和 *分别表示各项指标之间相关性达极显著( P<0.01)和显著( P<0.05)水平。
    Note: ** and * mean extremely significant at P<0.01, and significant at P<0.05, respectively.
    下载: 导出CSV
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  • 收稿日期:  2023-02-15
  • 录用日期:  2023-09-07
  • 修回日期:  2023-08-29
  • 网络出版日期:  2023-10-25
  • 刊出日期:  2023-09-28

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