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福建省山药初级核心种质的构建

张武君 陈菁瑛 刘保财 赵云青 黄颖桢

张武君,陈菁瑛,刘保财,等. 福建省山药初级核心种质的构建 [J]. 福建农业学报,2023,38(11):1267−1276 doi: 10.19303/j.issn.1008-0384.2023.11.002
引用本文: 张武君,陈菁瑛,刘保财,等. 福建省山药初级核心种质的构建 [J]. 福建农业学报,2023,38(11):1267−1276 doi: 10.19303/j.issn.1008-0384.2023.11.002
ZHANG W J, CHEN J Y, LIU B C, et al. Establishing a Primary Core Collection of Chinese Yam Germplasms in Fujian [J]. Fujian Journal of Agricultural Sciences,2023,38(11):1267−1276 doi: 10.19303/j.issn.1008-0384.2023.11.002
Citation: ZHANG W J, CHEN J Y, LIU B C, et al. Establishing a Primary Core Collection of Chinese Yam Germplasms in Fujian [J]. Fujian Journal of Agricultural Sciences,2023,38(11):1267−1276 doi: 10.19303/j.issn.1008-0384.2023.11.002

福建省山药初级核心种质的构建

doi: 10.19303/j.issn.1008-0384.2023.11.002
基金项目: 福建省农业种质资源创新专项(ZZZYCXZX202208);福建省药用植物种质资源库(圃)(ZYBHDWZX202203);福建省农业高质量发展超越“5511”协同创新工程项目(XTCXGC2021003-5);福建省农业科学院药用植物科技创新团队建设项目(CXTD2021014-2);福建省农业科学院生产性工程化实验室建设项目(2015GCH-6)
详细信息
    作者简介:

    张武君(1988 —),女,硕士,助理研究员,主要从事药用植物生理生化研究,E-mail:352047618@qq.com

    通讯作者:

    陈菁瑛 (1966 —),女,研究员,主要从事药用植物资源利用研究,E-mail:cjy6601@163.com

  • 中图分类号: S632.1

Establishing a Primary Core Collection of Chinese Yam Germplasms in Fujian

  • 摘要:   目的  构建福建省山药初级核心种质,为福建地方山药资源的保存、品种选育提供理论依据。  方法  以55份福建省地方主栽山药资源为材料,调查20个农艺性状,包括7个数量性状和13个描述型性状,采用离差平方和法进行聚类,根据聚类结果采用优先取样法构建初级核心种质,同时采用统计学方法及ISSR分子标记等对其代表性进行评价。  结果  构建的初级核心种质数量占原种质的43.6%,在降低冗余的同时保留了选育品种和有突出特点或地方特色的资源。初级核心种质和原种质各性状的均值、方差、变异系数、香农多样性指数无显著性差异。数量性状除茎粗外,核心种质保留了原种质变异范围的84.9%~100%。描述性指标除最弱的生长势外,核心种质保留了原种质的全部等级分布。初级核心种质和原种质的ISSR分子标记的等位基因数量、有效等位基因数量、香农多样性信息指数、Nei’s基因多样性指数无显著差异,且初级核心种质的多态位点保留率达到原种质的98.6%。基于农艺性状的主成分分析和基于ISSR分子标记的主坐标分析均确认了核心种质的代表性。  结论  构建的福建省山药初级核心种质能够较好地代表原种质的遗传多样性,有助于福建地方山药资源的保存和开发利用。
  • 图  1  55份福建山药资源农艺性状聚类

    Figure  1.  Phenotypic traits clustering on 55 Chinese yam germplasms in Fujian

    图  2  山药初级核心种质与保留种质的主坐标分布

    Figure  2.  Main coordinate distribution of PCCCY and preserved Chinese yam germplasm collection

    表  1  55份山药资源编号、名称和基原

    Table  1.   Codes, names, and origins of 55 Chinese yam germplasms

    编号
    No.
    种质资源名称
    Germplasm resource name
    基原
    Origins
    编号
    No.
    种质资源名称
    Germplasm resource name
    基原
    Origins
    1 麻沙硬壳薯
    Masha hard-shelled yam
    褐苞薯蓣
    D. persimilis
    29 宁化三黄村徐引2号
    Ninghua Sanhuang Xu Yin No.2
    褐苞薯蓣
    D. persimilis
    2 麻沙土薯
    Masha local yam
    褐苞薯蓣
    D. persimilis
    30 清流雪薯*
    Qingliu snow yam
    褐苞薯蓣
    D. persimilis
    3 麻沙小叶永安薯
    Masha small-leaf yong'an yam
    褐苞薯蓣
    D. persimilis
    31 明溪土薯
    Mingxi local yam
    褐苞薯蓣
    D. persimilis
    4 麻沙六月薯*
    Mosha June yam
    薯蓣
    D. opposita
    32 明溪红皮白肉
    Mingxi red skin white pulp
    参薯
    D. alata
    5 麻沙七月薯*
    Mosha July yam
    薯蓣
    D. opposita
    33 明溪淮山1号*
    Mingxi Huaishan No.1
    褐苞薯蓣
    D. persimilis
    6 麻沙1号山药*
    Masha No.1 yam
    褐苞薯蓣
    D. persimilis
    34 安砂小叶薯*
    Ansha small-leaf yam
    褐苞薯蓣
    D. persimilis
    7 麻沙大薯
    Masha big yam
    参薯
    D. alata
    35 安砂大叶薯*
    Ansha large-leaf yam
    参薯
    D. alata
    8 麻沙紫薯*
    Masha purple yam
    参薯
    D. alata
    36 建宁紫山药*
    Jianning purple yam
    参薯
    D. alata
    9 麻沙大叶永安薯
    Masha large-leaf yong'an yam
    褐苞薯蓣
    D. persimilis
    37 三明三元紫山药
    Sanming Sanyuan purple yam
    参薯
    D. alata
    10 麻沙江西薯
    Mashajiang sweet yam
    山薯
    D. fordii
    38 马铺1号山药*
    Mapu No.1 yam
    山薯
    D. fordii
    11 浦城紫山药
    Pucheng purple yam
    参薯
    D. alata
    39 屏南麻沙薯
    Pingnan Masha yam
    褐苞薯蓣
    D. persimilis
    12 连城紫山药
    Liancheng purple yam
    参薯
    D. alata
    40 屏南牛腿薯
    Pingnan beef leg yam
    参薯
    D. alata
    13 连城红皮白肉*
    Liancheng red skin white pulp
    参薯
    D. alata
    41 屏南棉薯*
    Pingnan soft yam
    薯蓣
    D. opposita
    14 宣和雪薯*
    Xuanhe snow yam
    褐苞薯蓣
    D. persimilis
    42 福安白石板大薯
    Fu'an Baishiban yam
    参薯
    D. alata
    15 新罗紫山药
    Xinluo purple yam
    参薯
    D. alata
    43 福安前洋糯米薯*
    Fu'an Qianyang yam
    参薯
    D. alata
    16 武平1号紫山药
    Wuping No.1 purple yam
    参薯
    D. alata
    44 永春紫山药
    Yongchun purple yam
    参薯
    D. alata
    17 武平2号紫山药*
    Wuping No.2 purple yam
    参薯
    D. alata
    45 永春锦溪村土薯
    Yongchun Jinxi local yam
    褐苞薯蓣
    D. persimilis
    18 长汀野药薯
    Changting wild yam
    山薯
    D. fordii
    46 安溪山格淮山*
    Anxi Shange Huaishan
    褐苞薯蓣
    D. persimilis
    19 长汀淮山*
    Changting Huaishan
    褐苞薯蓣
    D. persimilis
    47 德化淮山
    Dehua Huaishan
    褐苞薯蓣
    D. persimilis
    20 长汀红皮白肉
    Changting red skin white pulp
    参薯
    D. alata
    48 德化紫薇紫山药
    Dehua Ziwei purple yam
    参薯
    D. alata
    21 长汀紫山药
    Changting purple yam
    参薯
    D. alata
    49 德化1号紫山药
    Dehua No.1 purple yam
    参薯
    D. alata
    22 长汀紫玉淮山
    Changting purple jade Huaishan
    参薯
    D. alata
    50 德化2号紫山药*
    Dehua No.2 purple yam
    参薯
    D. alata
    23 宁化石寮村土薯
    Ninghua Shiliao local yam
    褐苞薯蓣
    D. persimilis
    51 德化芹峰淮山*
    Dehua Qinfeng Huaishan
    褐苞薯蓣
    D. persimilis
    24 宁化早熟
    Ninghua precocious yam
    褐苞薯蓣
    D. persimilis
    52 马铺3号*
    Mapu No.3 yam
    参薯
    D. alata
    25 宁化晚熟*
    Ninghua late maturation yam
    褐苞薯蓣
    D. persimilis
    53 马铺6号紫山药*
    Mapu No.6 purple yam
    参薯
    D. alata
    26 宁化三黄村奶薯
    Ninghua Sanhuang milk yam
    褐苞薯蓣
    D. persimilis
    54 马铺日本薯蓣*
    Mapu Japanese yam
    日本薯蓣 D.japonica
    27 宁化三黄村紫薯
    Ninghua Sanhuang purple yam
    参薯
    D. alata
    55 马铺7号紫山药*
    Mapu No.7 purple yam
    参薯
    D. alata
    28 宁化三黄村徐引1号
    Ninghua Sanhuang Xu Yin No.1
    褐苞薯蓣
    D. persimilis
    *表示初级核心种质资源。
    * indicates primary core collection.
    下载: 导出CSV

    表  2  描述性指标的分级和赋值

    Table  2.   Grading and assignments of descriptive traits

    性状
    Characters
    分级与赋值
    Grading and assignment
    生长势 GP1=弱Weak;2=较弱Relatively weak;3=中Mdium;4=较强Relatively strong;5=强Strong
    茎蔓狭翅 SNW1=无No;2=黄绿色Yellow-green;3=紫色 Purple
    基部茎刺 SBT1=无No;2=有Yes
    叶柄近叶端颜色 PLC1=黄绿色Yellow-green;2=紫色 Purple
    叶形 LS1=戟形Halberd shape;2=长心形Long heart shape;3=心形Heart shape;4=披针形Lanceolate
    叶面网脉 LSRV1=不明显Inapparent;2=明显Apparent
    零余子 Bulbil1=无No;2=有Yes
    开花 Flowering1=无No;2=有Yes
    块茎形状 TS1=短棒状Short bar shape;2=长棒状Long bar shape;3=短圆柱形Short cylindrical shape;4=长圆柱形Long cylindrical shape;5=不规则块状 Irregular shape
    表皮光滑度 SS1=光滑Smooth;2=较光滑Relatively smooth;3=较粗糙Relatively rough;4=粗糙Rough
    须根数 FRN1=少Only a little;2=较少Not many;3=多many
    块茎肉色 TFC1=白White;2=黄白Yellow with white;3=全紫色Purple;4=不均匀紫色Uneven purple;5=仅内皮层紫色Only the endothelium is purple
    肉质褐变性 FB1=易Easy;2=不易Hard
    下载: 导出CSV

    表  3  原种质与初级核心种质7个数量性状的平均值、方差、极差和变异系数的比较

    Table  3.   Mean, variance, range, and CV of 7 quantitative traits of previous collection and PCCCY

    性状
    Characters
    平均值 Mean方差 Variance极差 Range变异系数 CV/%
    原种质 OC初级核心种质 PCC原种质 OC初级核心种质 PCC原种质 OC初级核心种质 PCC原种质 OC初级核心种质 PCC
    茎粗SD/mm 3.223.270.630.652.1~6.32.1~5.024.5824.73
    叶长LL/cm 14.6313.889.4110.816.6-21.26.6~19.020.9720.49
    叶宽LW/cm 8.398.354.486.153.0~13.63.0~13.625.2324.30
    块茎长TL cm50.2954.13483.01552.6916.4~97.420.5~97.443.7043.43
    块茎直径TD/mm 65.0167.851641.532207.235.2~230.032~23062.3269.25
    龙头长TTL/cm 5.435.8717.6821.690~15.60~15.677.3979.30
    块茎鲜重TFW/g 917.00984.77366456.52451101.78262.5~3111.7262.53~2813.766.0168.20
    下载: 导出CSV

    表  4  原种质与初级核心种质13个描述型性状的等级分布和香农指数的比较

    Table  4.   Grade distribution of 13 descriptive traits and Shannon-Weaver indexes of PCCCY and previous collection

    性状
    Characters
    等级分布
    Grade distribution
    香浓多样性指数
    Shannon-Weaver index
    原种质
    OC
    初级核心种质
    PCC
    原种质
    OC
    初级核心种质
    PCC
    生长势 GP1~52-51.111.01
    茎蔓狭翅 SNW1~31~30.980.96
    基部茎刺 SBT1~21~20.210.17
    叶柄近叶端颜色 PLC1~21~20.680.64
    叶形 LS1~41~40.851.12
    叶面网脉 LSRV1~21~20.670.66
    零余子有无 BON1~21~20.520.60
    开花与否 FON1~21~20.690.68
    块茎形状 TS1~51~51.481.51
    表皮光滑度 SS1~41~40.970.92
    须根数 FRN1~41~41.241.32
    块茎肉色 TFC1~51~51.271.23
    肉质褐变性 FB1~21~2 0.260.29
    下载: 导出CSV

    表  5  供试ISSR引物及其扩增的多态性

    Table  5.   Polymorphisms and amplified products of ISSR primers

    编号
    No.
    引物名称
    Primer name
    引物序列
    Primer sequences
    多态位点
    Polymorphic sites
    总位点
    Total sites
    多态性比率
    Polymorphism ratio/%
    1UBC807AGA GAG AGA GAG AGA GT161794.12
    2UBC808AGA GAG AGA GAG AGA GC121485.71
    3UBC810GAG AGA GAG AGA GAG AT141593.33
    4UBC811GAG AGA GAG AGA GAG AC1414100.00
    5UBC818CAC ACA CAC ACA CAC AG1616100.00
    6UBC825ACA CAC ACA CAC ACA CT1414100.00
    7UBC827ACA CAC ACA CAC ACA CG1616100.00
    8UBC834AGA GAG AGA GAG AGA GYT1212100.00
    9UBC856ACA CAC ACA CAC ACA CYA1717100.00
    10UBC864ATG ATG ATG ATG ATG ATG1414100.00
    平均 Average14.514.997.32
    下载: 导出CSV

    表  6  原种质与初级核心种质的分子遗传多样性参数比较

    Table  6.   Grade distribution on molecular genetic diversity parameters of PCCCY and previous collection

    遗传信息
    genetic information
    样本数
    Sample size
    等位基因数Na
    有效等位基因数Ne多样性指数He香农多样性信息指数I多态位点
    Polymorphic sites
    多态位点率
    Polymorphic site rate /%
    原种质 OC551.9732± 0.16221.6015±0.32390.3461±0.14880.5147±0.187114597.32
    初级核心种质 PCC241.9597± 0.19731.6288±0.31180.3594±0.14250.5307±0.181014395.97
    下载: 导出CSV

    表  7  原种质和初级核心种质主成分分析的特征值及累计贡献率

    Table  7.   Characteristic values and cumulative contribution rates of PCCCY and previous collection based on principal component analysis

    主成份
    Main component
    原种质 OC初级核心种质 PCC
    特征值
    Eigenvalues
    贡献率
    Contribution rate/%
    累积贡献率
    Cumulative contribution rate/%
    特征值
    Eigenvalues
    贡献率
    Contribution rate/%
    累积贡献率
    Cumulative contribution rate/%
    19.16345.81445.8149.37846.89046.890
    22.75613.78059.5953.17615.87962.769
    32.28411.41971.0141.9549.77272.541
    41.4567.28278.2961.3736.86579.406
    51.0135.06383.3591.1305.64985.055
    下载: 导出CSV
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  • 收稿日期:  2023-08-30
  • 修回日期:  2023-10-03
  • 网络出版日期:  2023-12-21
  • 刊出日期:  2023-11-28

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