• 中文核心期刊
  • CSCD来源期刊
  • 中国科技核心期刊
  • CA、CABI、ZR收录期刊

留言板

尊敬的读者、作者、审稿人, 关于本刊的投稿、审稿、编辑和出版的任何问题, 您可以本页添加留言。我们将尽快给您答复。谢谢您的支持!

姓名
邮箱
手机号码
标题
留言内容
验证码

老鼠簕真菌病病原的分离鉴定及生物学特性

马林 袁宗胜 郑瑜婷 郑雄 陈维峰 王思凡 谢宝贵 刘芳

马林,袁宗胜,郑瑜婷,等. 老鼠簕真菌病病原的分离鉴定及生物学特性 [J]. 福建农业学报,2023,38(11):1329−1336 doi: 10.19303/j.issn.1008-0384.2023.11.009
引用本文: 马林,袁宗胜,郑瑜婷,等. 老鼠簕真菌病病原的分离鉴定及生物学特性 [J]. 福建农业学报,2023,38(11):1329−1336 doi: 10.19303/j.issn.1008-0384.2023.11.009
MA L, YUAN Z S, ZHENG Y T, et al. Isolation, Identification, and Biological Characteristics of a Fungal Pathogen on Acanthus ilicifolius [J]. Fujian Journal of Agricultural Sciences,2023,38(11):1329−1336 doi: 10.19303/j.issn.1008-0384.2023.11.009
Citation: MA L, YUAN Z S, ZHENG Y T, et al. Isolation, Identification, and Biological Characteristics of a Fungal Pathogen on Acanthus ilicifolius [J]. Fujian Journal of Agricultural Sciences,2023,38(11):1329−1336 doi: 10.19303/j.issn.1008-0384.2023.11.009

老鼠簕真菌病病原的分离鉴定及生物学特性

doi: 10.19303/j.issn.1008-0384.2023.11.009
基金项目: 国家自然科学基金项目(32002108);闽江学院引进人才预研项目(33004328)
详细信息
    作者简介:

    马林(1997 — ),男,硕士研究生,主要从事食用菌和农业微生物研究,E-mail:worldmalinmm@gmail.com

    通讯作者:

    刘芳(1980 — ),女,讲师,主要从事食用菌和农业微生物研究,E-mail:fjliufang@163.com

  • 中图分类号: S436

Isolation, Identification, and Biological Characteristics of a Fungal Pathogen on Acanthus ilicifolius

  • 摘要:   目的  明确老鼠簕(Acanthus ilicifolius)叶斑病的病原种类及其生物学特性。   方法  从福建省漳江口红树林国家级自然保护区采集病叶,采用离体组织分离法对病叶病原菌进行分离,采用形态学与分子生物学(ITS和EF1-α基因序列分析)的方法鉴定其病原菌种类,并对该病原菌的菌丝在不同温度、pH、碳源和氮源等条件下的生物学特性进行研究,以及对过氧化氢酶(CAT),超氧化物歧化酶(SOD)、过氧化物酶(POD)、漆酶、纤维素酶(CL)、β-葡萄糖苷酶(β-GC)进行活性检测。   结果  经鉴定该病原菌为真菌界子囊菌门镰刀菌科的轮枝镰刀菌(Fusarium verticillioides)。这是轮枝镰刀菌侵染老鼠簕引发叶斑病的首次报道。生物学特性研究显示该病原菌生长的最适温度25 ℃,最适pH为10,最佳碳源为淀粉,最佳氮源为酵母粉。3种氧化还原酶中,过氧化物酶(POD)活性最高;3种水解酶中,纤维素酶(CL)活性最高。   结论  分离鉴定了福建省漳江口老鼠簕叶斑病病原菌为轮枝镰刀菌(Fusarium verticillioides),明确了病原菌的生物学特性。
  • 图  1  老鼠簕叶斑病症状

    A为自然发病叶片症状;B为室内离体接种后叶片正面症状;C为室内离体接种后叶片背面症状。

    Figure  1.  Symptoms on A. ilicifolius infected by leaf spot disease

    A: Symptoms on diseased leaves in nature; B: symptoms shown on face of diseased leaf after in vitro inoculation; C: symptoms shown on back of diseased leaf after in vitro inoculation.

    图  2  老鼠簕叶斑病病原菌形态特征

    A为菌落的正面形态;B为菌丝的圆形缠绕形态;C为孢子聚集结构;D为小分生孢子;E为大分生孢子;F为厚垣孢子。

    Figure  2.  Morphology of pathogen causing leaf spot disease on A. ilicifolius

    A: Top view of fungal colony; B: circular winding mycelial growth; C: aggregated spores; D: microconidia; E: macroconidia; F: chlamydospore.

    图  3  基于EF-1α基因序列的系统发育树

    Figure  3.  Phylogenetic tree based on EF-1α gene sequences

    图  4  基于ITS基因序列的系统发育树

    Figure  4.  Phylogenetic tree based on ITS gene sequences

    图  5  病原菌在不同温度的生长情况

    在显著性水平为0.05的情况下,不同小写字母表示显著差异。下同。

    Figure  5.  Growth of pathogen at different temperatures

    Data with different lowercase letters indicate significant differences at level of 0.05. Same for below.

    图  6  病原菌在不同pH的生长情况

    Figure  6.  Growth of pathogen at different pH

    图  7  病原菌在不同碳源的生长情况

    Figure  7.  Growth of pathogen on different carbon sources

    图  8  病原菌在不同氮源的生长情况

    Figure  8.  Growth of pathogen on different nitrogen sources

    图  9  病原菌的氧化还原酶活性

    Figure  9.  Oxidoreductase activity of pathogen

    图  10  病原菌的水解酶活性

    Figure  10.  Hydrolase activity of pathogen

  • [1] 黄源欣, 辛琨, 熊燕梅, 等. 基于多环境因子条件下老鼠簕的生长适应性 [J]. 中南林业科技大学学报, 2023, 43(5):86−94.

    HUANG Y X, XIN K, XIONG Y M, et al. Growth adaptability of Acanthus ilicifolius based on multiple environmental factors [J]. Journal of Central South University of Forestry & Technology, 2023, 43(5): 86−94.(in Chinese)
    [2] DAHDOUH-GUEBAS F. World atlas of mangroves: Mark spalding, mami kainuma and lorna collins (eds) [J]. Human Ecology, 2011, 39(1): 107−109. doi: 10.1007/s10745-010-9366-7
    [3] 徐慧鹏, 刘涛, 张建兵. 红树林碳埋藏过程对海平面上升、气候变化和人类活动的响应 [J]. 广西科学, 2020, 27(1):84−90.

    XU H P, LIU T, ZHANG J B. Response of carbon sequestration in mangroves to sea level rise, climate change and human activities [J]. Guangxi Sciences, 2020, 27(1): 84−90.(in Chinese)
    [4] 许方宏, 张进平, 张倩媚, 等. 广东湛江高桥三个天然红树林的土壤碳库 [J]. 价值工程, 2012, 31(15):5−6.

    XU F H, ZHANG J P, ZHANG Q M, et al. Carbon storage of three natural mangrove forests in Gaoqiao, Zhanjiang [J]. Value Engineering, 2012, 31(15): 5−6.(in Chinese)
    [5] 但新球, 廖宝文, 吴照柏, 等. 中国红树林湿地资源、保护现状和主要威胁 [J]. 生态环境学报, 2016, 25(7):1237−1243.

    DAN X Q, LIAO B W, WU Z B, et al. Resources, conservation status and main threats of mangrove wetlands in China [J]. Ecology and Environmental Sciences, 2016, 25(7): 1237−1243.(in Chinese)
    [6] 韦秀桂, 王红园, 黄秀昆, 等. 老鼠簕生物碱A对小鼠药物性肝损伤的保护作用 [J]. 中国实验方剂学杂志, 2019, 25(15):103−107.

    WEI X G, WANG H Y, HUANG X K, et al. Protective effect of alkaloid A from acanthi ilicifolii herba seu Radix on drug-induced liver injury in mice [J]. Chinese Journal of Experimental Traditional Medical Formulae, 2019, 25(15): 103−107.(in Chinese)
    [7] 王宗英, 陈永好, 郑广进, 等. 老鼠簕生物碱A及其衍生物乙酰老鼠簕生物碱A的抗炎镇痛作用 [J]. 中国医院药学杂志, 2011, 31(10):807−810.

    WANG Z Y, CHEN Y H, ZHENG G J, et al. Study on the anti-inflammatory and analgesic effects of ilicifolius alkaloids A and its derivatives acetyl ilicifolius alkaloids A [J]. Chinese Journal of Hospital Pharmacy, 2011, 31(10): 807−810.(in Chinese)
    [8] SARDAR P K, DEV S, AL BARI M A, et al. Antiallergic, anthelmintic and cytotoxic potentials of dried aerial parts of Acanthus ilicifolius L [J]. Clinical Phytoscience, 2018, 4(1): 1−13. doi: 10.1186/s40816-018-0060-4
    [9] 李培谦, 冯宝珍, 赵燕飞, 等. 芍药红斑病病原菌鉴定、生物学特性及有效药剂筛选 [J]. 核农学报, 2023, 37(8):1533−1541.

    LI P Q, FENG B Z, ZHAO Y F, et al. Identification, biological characteristics and screening of effective fungicides for red spot on peony [J]. Journal of Nuclear Agricultural Sciences, 2023, 37(8): 1533−1541.(in Chinese)
    [10] 范绍斌, 苏济钰, 方甜, 等. 枫香叶斑病病原菌 [J]. 菌物学报, 2022, 41(3):420−434.

    FAN S B, SU J Y, FANG T, et al. Pathogen of Liquidambar formosana leaf spot [J]. Mycosystema, 2022, 41(3): 420−434.(in Chinese)
    [11] 何苏琴, 文朝慧, 白滨, 等. 莴苣叶疫病和茎腐病的病原 [J]. 菌物学报, 2023, 42(2):468−483.

    HE S Q, WEN Z H, BAI B, et al. Leaf blight and stem rot caused by Pythium tracheiphilum on lettuces in Gansu and Qinghai Provinces, China [J]. Mycosystema, 2023, 42(2): 468−483.(in Chinese)
    [12] APPLEBY J M, NELSON G, MCPHERSON M J, et al. Polymerase chain reaction amplification of the RuBisCo small subunit genes and their novel application to plant tissue identification [J]. Heredity, 1997, 79(6): 557−563. doi: 10.1038/hdy.1997.199
    [13] 谭秀梅, 阿地力·沙塔尔, 朴春根, 等. 无柄灵芝遗传多样性的SRAP、ITS、TEF1-α和LSU分析 [J]. 微生物学通报, 2016, 43(12):2667−2677.

    TAN X M, ADIL·SATTAR, PIAO C G, et al. Genetic diversity of Ganoderma resinaceum by SRAP, ITS, TEF1-α and LSU markers [J]. Microbiology China, 2016, 43(12): 2667−2677.(in Chinese)
    [14] 方中达. 植病研究方法[M]. 3版. 北京: 中国农业出版社, 1998.
    [15] 普梅英, 武自强, 张诗文, 等. 华东山茶花腐病病原菌分离鉴定 [J]. 浙江农业学报, 2023, 35(1):121−127.

    PU M Y, WU Z Q, ZHANG S W, et al. Isolation and identification of petal blight disease of Camellia japonica [J]. Acta Agriculturae Zhejiangensis, 2023, 35(1): 121−127.(in Chinese)
    [16] 朱轶慧, 刘玉军, 毕飞虎, 等. 红叶石楠叶斑病病原鉴定及其生物学特性研究 [J]. 植物保护, 2021, 47(5):210−215,235.

    ZHU Y H, LIU Y J, BI F H, et al. Identification and biological characteristics of the pathogen causing leaf spot disease on Photinia × fraseri [J]. Plant Protection, 2021, 47(5): 210−215,235.(in Chinese)
    [17] JOHNSON-BUCK A, LI J M, TEWARI M, et al. A guide to nucleic acid detection by single-molecule kinetic fingerprinting [J]. Methods, 2019, 153: 3−12. doi: 10.1016/j.ymeth.2018.08.002
    [18] BONNY S Q, MOTALIB HOSSAIN M A, UDDIN S M K, et al. Current trends in polymerase chain reaction based detection of three major human pathogenic vibrios [J]. Critical Reviews in Food Science and Nutrition, 2022, 62(5): 1317−1335. doi: 10.1080/10408398.2020.1841728
    [19] POCZAI P, VARGA I, HYVÖNEN J. Internal transcribed spacer (ITS) Evolution in Populations Of The Hyperparasitic European Mistletoe Pathogen Fungus, Sphaeropsis Visci (Botryosphaeriaceae): The utility of ITS2 secondary structures [J]. Gene, 2015, 558(1): 54−64. doi: 10.1016/j.gene.2014.12.042
    [20] 黎肇家, 李汶晋, 毛跃彦, 等. 栾树枝枯病病原菌鉴定及其生物学特性研究 [J]. 西北农林科技大学学报(自然科学版), 2020, 48(9):53−63.

    LI Z J, LI W J, MAO Y Y, et al. Identification and biological characteristics of branch withered disease causing pathogen to Koelreuteria paniculata Laxm [J]. Journal of Northwest A & F University (Natural Science Edition), 2020, 48(9): 53−63.(in Chinese)
    [21] WANG J, YU X P, JIA R, et al. An in vitro and in silico study to explore the response of catalase to 4-chlorophenol and their interacting mechanisms [J]. Journal of Molecular Liquids, 2021, 337: 116444. doi: 10.1016/j.molliq.2021.116444
    [22] WANG H, YANG H S, LIU J H, et al. Combined effects of temperature and copper ion concentration on the superoxide dismutase activity in Crassostrea ariakensis [J]. Acta Oceanologica Sinica, 2016, 35(4): 51−57. doi: 10.1007/s13131-016-0838-5
    [23] 白一彤, 张应朝. 氧化石墨烯对辣根过氧化物酶酶活的影响 [J]. 广东化工, 2018, 45(4):20−21.

    BAI Y T, ZHANG Y C. Influence of graphene oxide on the activity of horseradish peroxidase [J]. Guangdong Chemical Industry, 2018, 45(4): 20−21.(in Chinese)
    [24] 田晓丽, 姜文侠, 张笑然, 等. 基于恒温催化反应过程中吸光度实时测定的漆酶活性快速测定 [J]. 食品研究与开发, 2022, 43(16):139−145.

    TIAN X L, JIANG W X, ZHANG X R, et al. Rapid detection method for laccase activity based on real-time absorbance measurement at constant temperature [J]. Food Research and Development, 2022, 43(16): 139−145.(in Chinese)
    [25] CAI L N, XU S N, LU T, et al. Salt-tolerant mechanism of marine Aspergillus niger cellulase cocktail and improvement of its activity [J]. Chinese Journal of Chemical Engineering, 2020, 28(4): 1120−1128. doi: 10.1016/j.cjche.2019.11.012
    [26] 王茜, 许光治. Leifsonia菌β-葡萄糖苷酶(Lf18920)的酶学性质研究及对藏红花素转化作用的初步研究 [J]. 中国食品添加剂, 2023, 34(2):11−18.

    WANG X, XU G Z. Enzymatic properties of Leifsonia β-glucosidase (Lf18920) and its degradation of crocin [J]. China Food Additives, 2023, 34(2): 11−18.(in Chinese)
    [27] 黄立飞, 刘伟明, 刘也楠, 等. 甘薯茎基部腐烂病调查及病原鉴定 [J]. 中国农学通报, 2019, 35(18):135−141.

    HUANG L F, LIU W M, LIU Y N, et al. Investigation of stem base rot of sweetpotato and the pathogeny identification [J]. Chinese Agricultural Science Bulletin, 2019, 35(18): 135−141.(in Chinese)
    [28] 陈宏州, 杨红福, 姚克兵, 等. 水稻恶苗病病原菌鉴定及室内药剂毒力测定 [J]. 植物保护学报, 2018, 45(6):1356−1366.

    CHEN H Z, YANG H F, YAO K B, et al. Identification of rice bakanae pathogens and indoor toxicity test of fungicides [J]. Journal of Plant Protection, 2018, 45(6): 1356−1366.(in Chinese)
    [29] 刘丽萍, 方鹏鹏. 马铃薯干腐病镰刀菌的分离及生物学特性研究 [J]. 陇东学院学报, 2017, 28(5):62−66.

    LIU L P, FANG P P. The isolation and biological characteristic of Fusarium associated with potato dry rot [J]. Journal of Longdong University, 2017, 28(5): 62−66.(in Chinese)
    [30] 柴海燕, 贾娇, 白雪, 等. 吉林省玉米穗腐病致病镰孢菌的鉴定与部分菌株对杀菌剂的敏感性 [J]. 中国农业科学, 2023, 56(1):64−78.

    CHAI H Y, JIA J, BAI X, et al. Identification of pathogenic Fusarium spp. causing maize ear rot and susceptibility of some strains to fungicides in Jilin Province [J]. Scientia Agricultura Sinica, 2023, 56(1): 64−78.(in Chinese)
    [31] 李孟滕, 王芯, 马晓冉, 等. 紫花苜蓿根腐病病原菌分离鉴定与致病性 [J]. 中国草地学报, 2022, 44(11):84−91.

    LI M T, WANG X, MA X R, et al. Isolation, identification and pathogenicity of pathogens causing alfalfa root rot [J]. Chinese Journal of Grassland, 2022, 44(11): 84−91.(in Chinese)
    [32] 郭强, 马文清, 陈海生, 等. 广西崇左市甘蔗梢腐病病原菌的分离鉴定及生物学特性测定 [J]. 南方农业学报, 2019, 50(8):1728−1734.

    GUO Q, MA W Q, CHEN H S, et al. Isolation identification and biological characteristics of the pathogen causing sugarcane pokkah boeng in Chongzuo, Guangxi [J]. Journal of Southern Agriculture, 2019, 50(8): 1728−1734.(in Chinese)
    [33] 高婧, 张园园, 王凯, 等. 向日葵枯萎病菌的分离鉴定及其生物学特性 [J]. 中国油料作物学报, 2016, 38(2):214−222.

    GAO J, ZHANG Y Y, WANG K, et al. Identification of sunflower wilt pathogen and its biological characteristics [J]. Chinese Journal of Oil Crop Sciences, 2016, 38(2): 214−222.(in Chinese)
    [34] 郑童童, 王宁, 李进, 等. 小麦镰刀菌叶枯病病原鉴定及生物学特性研究 [J]. 中国农学通报, 2023, 39(8):90−98.

    ZHENG T T, WANG N, LI J, et al. Pathogen identification and biological characteristics of wheat Fusarium leaf blight [J]. Chinese Agricultural Science Bulletin, 2023, 39(8): 90−98.(in Chinese)
    [35] 覃茜, 陆祖正, 丁丽琼, 等. 广西兰花镰刀菌叶斑病病原菌鉴定及生物学特性 [J]. 北方园艺, 2019(18):40−46.

    QIN Q, LU Z Z, DING L Q, et al. Identification and biological characteristics of the pathogen causing orchid Fusarium leaf spot in Guangxi [J]. Northern Horticulture, 2019(18): 40−46.(in Chinese)
    [36] LI L J, LIU X, SHI Y X, et al. Synthesis of Fe3+-doped aminated lignin as A peroxidase mimic for colorimetric detection of H2O2 and glucose [J]. Paper and Biomaterials, 2021, 6(4): 1−11.
  • 加载中
图(10)
计量
  • 文章访问数:  260
  • HTML全文浏览量:  142
  • PDF下载量:  40
  • 被引次数: 0
出版历程
  • 收稿日期:  2023-07-22
  • 修回日期:  2023-08-16
  • 网络出版日期:  2023-12-21
  • 刊出日期:  2023-11-28

目录

    /

    返回文章
    返回