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高温胁迫对灰茶尺蠖生命参数及保护酶活性的影响

乔利 张天海 耿书宝 金银利 张方梅 武予清 潘鹏亮

乔利,张天海,耿书宝,等. 高温胁迫对灰茶尺蠖生命参数及保护酶活性的影响 [J]. 福建农业学报,2023,38(11):1337−1343 doi: 10.19303/j.issn.1008-0384.2023.11.010
引用本文: 乔利,张天海,耿书宝,等. 高温胁迫对灰茶尺蠖生命参数及保护酶活性的影响 [J]. 福建农业学报,2023,38(11):1337−1343 doi: 10.19303/j.issn.1008-0384.2023.11.010
QIAO L, ZHANG T H, GENG S B, et al. Effects of High Temperature on Lifespan, Fecundity, and Protective Enzymes of Ectropis grisescens [J]. Fujian Journal of Agricultural Sciences,2023,38(11):1337−1343 doi: 10.19303/j.issn.1008-0384.2023.11.010
Citation: QIAO L, ZHANG T H, GENG S B, et al. Effects of High Temperature on Lifespan, Fecundity, and Protective Enzymes of Ectropis grisescens [J]. Fujian Journal of Agricultural Sciences,2023,38(11):1337−1343 doi: 10.19303/j.issn.1008-0384.2023.11.010

高温胁迫对灰茶尺蠖生命参数及保护酶活性的影响

doi: 10.19303/j.issn.1008-0384.2023.11.010
基金项目: 河南省重点研发与推广专项(科技攻关)(222102110109);国家重点研发计划(2017YFD0200907);河南省青年科学基金项目(212300410229);信阳农林学院科技创新团队项目(XNKJTD-007)
详细信息
    作者简介:

    乔利(1980 — ),女,博士,副教授,主要从事农业昆虫与害虫防治研究,E-mail:2017180004@xyafu.edu.cn

    通讯作者:

    潘鹏亮(1976 — )男,博士,副教授,主要从事昆虫与害虫防治研究,E-mail:2014180001@xyafu.edu.cn

  • 中图分类号: S433.3

Effects of High Temperature on Lifespan, Fecundity, and Protective Enzymes of Ectropis grisescens

  • 摘要:   目的  明确灰茶尺蠖(Ectropis grisescens Warren)对高温的耐受性及其体内抗氧化系统对高温胁迫的响应机制。  方法  以25 ℃为对照,灰茶尺蠖雌、雄成虫经高温 30、35、38 ℃和41 ℃处理1 h,44 ℃处理3 min后,测定其寿命、产卵量、卵孵化率及其体内总蛋白含量、超氧化物歧化酶(Superoxide dismutas,SOD)、过氧化氢酶(Catalase,CAT)、过氧化物酶(Peroxidase,POD)的活性和总抗氧化(Total antioxidant capacity,T-AOC)能力的变化。  结果  随着胁迫温度的增加,灰茶尺蠖雌、雄成虫的寿命缩短,雌成虫的产卵量逐渐下降,卵孵化率降低。41 ℃和44 ℃时,卵孵化率为0。灰茶尺蠖雌、雄成虫总蛋白含量分别在35 ℃和38 ℃时达最高值,均显著高于其他处理,且雌、雄间差异显著。雌成虫SOD活性呈“低-高-低”的趋势,雄成虫SOD活性41 ℃处理较低。雌成虫在44 ℃时CAT活性最高,T-AOC含量随温度的增加逐渐降低,雌成虫的POD活性随温度升高逐渐增加,雄成虫呈“低-高-低”的趋势。  结论  灰茶尺蠖体内保护酶活性在适应高温胁迫过程中发挥了重要作用。
  • 图  1  温度胁迫对灰茶尺蠖的产卵量的影响

    不同小写字母表示不同温度处理间的测定值达显著差异(P<0.05)。图2~8同。

    Figure  1.  Effect of high temperature on oviposition of E. grisescens

    Data with different lowercase letters indicate significant differences in measured values at different temperature treatments (P<0.05). Same for Figures 2-8.

    图  2  温度胁迫后灰茶尺蠖的孵化率的影响

    Figure  2.  Effect of high temperature on hatchability of E. grisescens eggs

    图  3  温度胁迫对灰茶尺蠖寿命的影响

    不同小写字母表示雌或雄成虫不同温度处理间的差异显著性(P<0.05)。不同大写字母表示同一温度雌、雄成虫处理间的差异显著性(P<0.05)。下图同。

    Figure  3.  Effect of high temperature on lifespan of E. grisescens

    Those with different lowercase letters indicate significant differences between male and female adults at differert temperature (P<0.05); those with different capital letters indicate significant difference between male and female adults at the same temperature (P<0.05). Same for below.

    图  4  温度胁迫对灰茶尺蠖总蛋白含量的影响

    Figure  4.  Effect of high temperature on total protein in E. grisescens

    图  5  温度胁迫对灰茶尺蠖SOD活性的影响

    Figure  5.  Effect of high temperature on SOD activity in E. grisescens

    图  6  温度胁迫对灰茶尺蠖CAT活性的影响

    Figure  6.  Effect of high temperature on CAT activity

    图  7  温度胁迫对灰茶尺蠖POD活性的影响

    Figure  7.  Effect of high temperature on POD activity in E. grisescens

    图  8  温度胁迫对灰茶尺蠖T-AOC的影响

    Figure  8.  Effect of high temperature on T-AOC in E. grisescens

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  • 收稿日期:  2023-06-06
  • 修回日期:  2023-07-31
  • 网络出版日期:  2023-12-21
  • 刊出日期:  2023-11-28

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