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高温胁迫对灰茶尺蠖生命参数及保护酶活性的影响

乔利 张天海 耿书宝 金银利 张方梅 武予清 潘鹏亮

乔利,张天海,耿书宝,等. 高温胁迫对灰茶尺蠖生命参数及保护酶活性的影响 [J]. 福建农业学报,2023,38(11):1337−1343 doi: 10.19303/j.issn.1008-0384.2023.11.010
引用本文: 乔利,张天海,耿书宝,等. 高温胁迫对灰茶尺蠖生命参数及保护酶活性的影响 [J]. 福建农业学报,2023,38(11):1337−1343 doi: 10.19303/j.issn.1008-0384.2023.11.010
QIAO L, ZHANG T H, GENG S B, et al. Effects of High Temperature on Lifespan, Fecundity, and Protective Enzymes of Ectropis grisescens [J]. Fujian Journal of Agricultural Sciences,2023,38(11):1337−1343 doi: 10.19303/j.issn.1008-0384.2023.11.010
Citation: QIAO L, ZHANG T H, GENG S B, et al. Effects of High Temperature on Lifespan, Fecundity, and Protective Enzymes of Ectropis grisescens [J]. Fujian Journal of Agricultural Sciences,2023,38(11):1337−1343 doi: 10.19303/j.issn.1008-0384.2023.11.010

高温胁迫对灰茶尺蠖生命参数及保护酶活性的影响

doi: 10.19303/j.issn.1008-0384.2023.11.010
基金项目: 河南省重点研发与推广专项(科技攻关)(222102110109);国家重点研发计划(2017YFD0200907);河南省青年科学基金项目(212300410229);信阳农林学院科技创新团队项目(XNKJTD-007)
详细信息
    作者简介:

    乔利(1980 — ),女,博士,副教授,主要从事农业昆虫与害虫防治研究,E-mail:2017180004@xyafu.edu.cn

    通讯作者:

    潘鹏亮(1976 — )男,博士,副教授,主要从事昆虫与害虫防治研究,E-mail:2014180001@xyafu.edu.cn

  • 中图分类号: S433.3

Effects of High Temperature on Lifespan, Fecundity, and Protective Enzymes of Ectropis grisescens

  • 摘要:   目的  明确灰茶尺蠖(Ectropis grisescens Warren)对高温的耐受性及其体内抗氧化系统对高温胁迫的响应机制。  方法  以25 ℃为对照,灰茶尺蠖雌、雄成虫经高温 30、35、38 ℃和41 ℃处理1 h,44 ℃处理3 min后,测定其寿命、产卵量、卵孵化率及其体内总蛋白含量、超氧化物歧化酶(Superoxide dismutas,SOD)、过氧化氢酶(Catalase,CAT)、过氧化物酶(Peroxidase,POD)的活性和总抗氧化(Total antioxidant capacity,T-AOC)能力的变化。  结果  随着胁迫温度的增加,灰茶尺蠖雌、雄成虫的寿命缩短,雌成虫的产卵量逐渐下降,卵孵化率降低。41 ℃和44 ℃时,卵孵化率为0。灰茶尺蠖雌、雄成虫总蛋白含量分别在35 ℃和38 ℃时达最高值,均显著高于其他处理,且雌、雄间差异显著。雌成虫SOD活性呈“低-高-低”的趋势,雄成虫SOD活性41 ℃处理较低。雌成虫在44 ℃时CAT活性最高,T-AOC含量随温度的增加逐渐降低,雌成虫的POD活性随温度升高逐渐增加,雄成虫呈“低-高-低”的趋势。  结论  灰茶尺蠖体内保护酶活性在适应高温胁迫过程中发挥了重要作用。
  • 图  1  温度胁迫对灰茶尺蠖的产卵量的影响

    不同小写字母表示不同温度处理间的测定值达显著差异(P<0.05)。图2~8同。

    Figure  1.  Effect of high temperature on oviposition of E. grisescens

    Data with different lowercase letters indicate significant differences in measured values at different temperature treatments (P<0.05). Same for Figures 2-8.

    图  2  温度胁迫后灰茶尺蠖的孵化率的影响

    Figure  2.  Effect of high temperature on hatchability of E. grisescens eggs

    图  3  温度胁迫对灰茶尺蠖寿命的影响

    不同小写字母表示雌或雄成虫不同温度处理间的差异显著性(P<0.05)。不同大写字母表示同一温度雌、雄成虫处理间的差异显著性(P<0.05)。下图同。

    Figure  3.  Effect of high temperature on lifespan of E. grisescens

    Those with different lowercase letters indicate significant differences between male and female adults at differert temperature (P<0.05); those with different capital letters indicate significant difference between male and female adults at the same temperature (P<0.05). Same for below.

    图  4  温度胁迫对灰茶尺蠖总蛋白含量的影响

    Figure  4.  Effect of high temperature on total protein in E. grisescens

    图  5  温度胁迫对灰茶尺蠖SOD活性的影响

    Figure  5.  Effect of high temperature on SOD activity in E. grisescens

    图  6  温度胁迫对灰茶尺蠖CAT活性的影响

    Figure  6.  Effect of high temperature on CAT activity

    图  7  温度胁迫对灰茶尺蠖POD活性的影响

    Figure  7.  Effect of high temperature on POD activity in E. grisescens

    图  8  温度胁迫对灰茶尺蠖T-AOC的影响

    Figure  8.  Effect of high temperature on T-AOC in E. grisescens

  • [1] 陈宗懋, 蔡晓明, 周利, 等. 中国茶园有害生物防控40年 [J]. 中国茶叶, 2020, 42(1):1−8.

    CHEN Z M, CAI X M, ZHOU L, et al. Developments on tea plant pest control in past 40 years in China [J]. China Tea, 2020, 42(1): 1−8.(in Chinese)
    [2] 王定锋, 李良德, 李慧玲, 等. 基于转录组数据高通量发掘灰茶尺蠖微卫星标记 [J]. 茶叶学报, 2021, 62(4):191−197.

    WANG D F, LI L D, LI H L, et al. High-throughput unveiling of microsatellite markers using Ectropis grisescens transcriptome [J]. Acta Tea Sinica, 2021, 62(4): 191−197.(in Chinese)
    [3] LI Z Q, CAI X M, LUO Z X, et al. Geographical distribution of Ectropis grisescens (Lepidoptera: Geometridae) and Ectropis obliqua in China and description of an efficient identification method [J]. Journal of Economic Entomology, 2019, 112(1): 277−283. doi: 10.1093/jee/toy358
    [4] 罗宗秀, 苏亮, 李兆群, 等. 灰茶尺蠖性信息素田间应用技术研究 [J]. 茶叶科学, 2018, 38(2):140−145.

    LUO Z X, SU L, LI Z Q, et al. Field application technology of sex pheromone on Ectropis grisescens [J]. Journal of Tea Science, 2018, 38(2): 140−145.(in Chinese)
    [5] MARNETT L J, RIGGINS J N, WEST J D. Endogenous generation of reactive oxidants and electrophiles and their reactions with DNA and protein [J]. The Journal of Clinical Investigation, 2003, 111(5): 583−593. doi: 10.1172/JCI200318022
    [6] 李慧, 郝德君, 徐天, 等. 高温胁迫对植食性昆虫影响研究进展 [J]. 南京林业大学学报(自然科学版), 2022, 46(6):215−224.

    LI H, HAO D J, XU T, et al. The effects of heat stress on herbivorous insects: An overview and future directions [J]. Journal of Nanjing Forestry University (Natural Sciences Edition), 2022, 46(6): 215−224.(in Chinese)
    [7] DUBOVSKIĬ I M, GRIZANOVA E V, CHERTKOVA E A, et al. Generation of reactive oxygen species and activity of antioxidants in larva hemolymph of Galleria mellonella (L. ) (lepidoptera: Piralidae) at development of process of encapsulation [J]. Zhurnal Evoliutsionnoi Biokhimii i Fiziologii, 2010, 46(1): 30−36.
    [8] 曹美琳, 陶晡, 刘顺, 等. 温度对二点委夜蛾实验种群的影响 [J]. 植物保护学报, 2012, 39(6):531−535.

    CAO M L, TAO B, LIU S, et al. Influence of temperature on experimental population of Athetis lepigone(Möschler) [J]. Journal of Plant Protection, 2012, 39(6): 531−535.(in Chinese)
    [9] MCDONALD J R, HEAD J, BALE J S, et al. Cold tolerance, overwintering and establishment potential of Thrips palmi [J]. Physiological Entomology, 2000, 25(2): 159−166. doi: 10.1046/j.1365-3032.2000.00179.x
    [10] GONZÁLEZ-TOKMAN D, CÓRDOBA-AGUILAR A, DÁTTILO W, et al. Insect responses to heat: Physiological mechanisms, evolution and ecological implications in a warming world [J]. Biological Reviews of the Cambridge Philosophical Society, 2020, 95(3): 802−821. doi: 10.1111/brv.12588
    [11] FILAZZOLA A, MATTER S F, MACIVOR J S. The direct and indirect effects of extreme climate events on insects [J]. The Science of the Total Environment, 2021, 769: 145161. doi: 10.1016/j.scitotenv.2021.145161
    [12] VIDAL M C, ANNEBERG T J, CURÉ A E, et al. The variable effects of global change on insect mutualisms [J]. Current Opinion in Insect Science, 2021, 47: 46−52. doi: 10.1016/j.cois.2021.03.002
    [13] BLOIS J L, ZARNETSKE P L, FITZPATRICK M C, et al. Climate change and the past, present, and future of biotic interactions [J]. Science, 2013, 341(6145): 499−504. doi: 10.1126/science.1237184
    [14] BALE J S. Insects and low temperatures: From molecular biology to distributions and abundance [J]. Philosophical Transactions of the Royal Society of London Series B, Biological Sciences, 2002, 357(1423): 849−62. doi: 10.1098/rstb.2002.1074
    [15] SINCLAIR B J, VERNON P, JACO KLOK C, et al. Insects at low temperatures: An ecological perspective [J]. Trends in Ecology & Evolution, 2003, 18(5): 257−262.
    [16] 蒋素容, 李冠烁, 李庆, 等. 温度对麻疯树柄细蛾生长发育和繁殖的影响 [J]. 中国森林病虫, 2012, 31(4):8−10,17.

    JIANG S R, LI G S, LI Q, et al. Effects of temperature on development and reproduction of Stomphastis thraustica Meyrick [J]. Forest Pest and Disease, 2012, 31(4): 8−10,17.(in Chinese)
    [17] 杜娟, 郭建挺, 张亚素, 等. 温度对梨小食心虫Grapholitha molesta Busck生长发育及繁殖的影响 [J]. 西北农业学报, 2009, 18(6):314−318.

    DU J, GUO J T, ZHANG Y S, et al. Effect of temperature on development and reproduction of Grapholitha molesta (Busck) (Lepidoptera: Tortricidae) [J]. Acta Agriculturae Boreali-Occidentalis Sinica, 2009, 18(6): 314−318.(in Chinese)
    [18] 邢俊, 李佳睿, 王洪平. 不同温度对桃小食心虫保护酶系活性的影响 [J]. 贵州农业科学, 2012, 40(6):107−109.

    XING J, LI J R, WANG H P. Effect of different temperature on the activity of protective enzymes of Carposina niponensis [J]. Guizhou Agricultural Sciences, 2012, 40(6): 107−109.(in Chinese)
    [19] 张芸, 钱秀娟, 姬国红, 等. 夜蛾斯氏线虫对黄粉虫三种保护酶活力的影响 [J]. 甘肃农业大学学报, 2010, 45(5):88−91,95.

    ZHANG Y, QIAN X J, JI G H, et al. Changes of the activities of protective enzymes in Tenebrio molitor infected by Steinernema feltiae [J]. Journal of Gansu Agricultural University, 2010, 45(5): 88−91,95.(in Chinese)
    [20] 周冬, 杜一民, 杨杰, 等. 连续多代UV-B照射对麦长管蚜保护酶活性的影响 [J]. 昆虫学报, 2014, 57(7):762−768.

    ZHOU D, DU Y M, YANG J, et al. Effects of UV-B radiation in successive generations on the activities of protective enzymes in the grain aphid, Sitobion avenae(Hemiptera: Aphididae) [J]. Acta Entomologica Sinica, 2014, 57(7): 762−768.(in Chinese)
    [21] 方程. 光照对梨小食心虫Grapholita molesta(Busck)主要生物学参数及保护酶活性的影响[D]. 杨凌: 西北农林科技大学, 2015.

    FANG C. Effects of different lights on main biological parameters and protective enzymes of Grapholita molesta(Lepidoptera: Tortricidae)[D]. Yangling: Northwest A & F University, 2015. (in Chinese)
    [22] 李品武, 付雪莲, 陈世春, 等. 温度胁迫对茶淡黄刺蛾四种保护酶活力和总抗氧化力的影响 [J]. 应用昆虫学报, 2016, 53(4):809−816. doi: 10.7679/j.issn.2095-1353.2016.04.015

    LI P W, FU X L, CHEN S C, et al. Effect of temperature stress on four protective enzymes and overall antioxidant capacity in Darna trima(Moore) [J]. Chinese Journal of Applied Entomology, 2016, 53(4): 809−816.(in Chinese) doi: 10.7679/j.issn.2095-1353.2016.04.015
    [23] 卢亚菲, 张祥, 王广, 等. 紫外辐射对三叶草彩斑蚜3种保护酶活性的影响 [J]. 草业科学, 2019, 36(5):1428−1434.

    LU Y F, ZHANG X, WANG G, et al. Effects of ultraviolet radiation on three types of protective enzymes in Therioaphis trifolii [J]. Pratacultural Science, 2019, 36(5): 1428−1434.(in Chinese)
    [24] 郭俊杰, 王勇, 季清娥, 等. 高温对阿里山潜蝇茧蜂体内保护酶系活性的影响 [J]. 热带作物学报, 2013, 34(6):1166−1169.

    GUO J J, WANG Y, JI Q E, et al. The influence of the activity of Fopius arisanus (sonan) protective enzyme system at high temperature [J]. Chinese Journal of Tropical Crops, 2013, 34(6): 1166−1169.(in Chinese)
    [25] 谢勤丹, 刘天宇, 王强, 等. 高温对感染Wolbachia米蛾体内保护酶和生殖的影响 [J]. 黑龙江农业科学, 2021(1):59−63.

    XIE Q D, LIU T Y, WANG Q, et al. Effects of high temperature on protective enzymes and reproduction in infected with Wolbachia [J]. Heilongjiang Agricultural Sciences, 2021(1): 59−63.(in Chinese)
    [26] 乔利, 秦道正, 卢兆成, 等. 温度对茶小绿叶蝉成虫存活率及保护酶系的影响 [J]. 植物保护学报, 2015, 42(2):223−228.

    QIAO L, QIN D Z, LU Z C, et al. Effects of temperature on survival rate and protection enzymes of Empoasca onukii Matsuda [J]. Journal of Plant Protection, 2015, 42(2): 223−228.(in Chinese)
    [27] 葛超美, 殷坤山, 唐美君, 等. 灰茶尺蠖发育起点温度和有效积温的研究 [J]. 植物保护, 2016, 42(6):110−112.

    GE C M, YIN K S, TANG M J, et al. Developmental threshold temperature and effective accumulated temperature of Ectropis grisescens [J]. Plant Protection, 2016, 42(6): 110−112.(in Chinese)
    [28] JAFARI S, FATHIPOUR Y, FARAJI F. Temperature-dependent development of Neoseiulus barkeri (Acari: Phytoseiidae) on Tetranychus urticae (Acari: Tetranychidae) at seven constant temperatures [J]. Insect Science, 2012, 19(2): 220−228. doi: 10.1111/j.1744-7917.2011.01444.x
    [29] 张天浩, 惠琴, 赵义涛, 等. 短时高温对绿豆象各虫态生长发育的影响 [J]. 应用昆虫学报, 2020, 57(2):363−370.

    ZHANG T H, HUI Q, ZHAO Y T, et al. Effects of short-term heat stress on different developmental stages of Callosobruchus chinensis(L. )(Coleoptera: Bruchidae) [J]. Chinese Journal of Applied Entomology, 2020, 57(2): 363−370.(in Chinese)
    [30] 崔娟, 乔方, 胡英露, 等. 高温胁迫对筛豆龟蝽成虫抗氧化能力的影响 [J]. 环境昆虫学报, 2021, 43(5):1244−1249.

    CUI J, QIAO F, HU Y L, et al. Antioxidant responses of Megacopta cribraria (Hemiptera: Plataspidae) adults exposed to high temperature stress [J]. Journal of Environmental Entomology, 2021, 43(5): 1244−1249.(in Chinese)
    [31] 向敏, 樊泰山, 扈鸿霞, 等. 短时高温对意大利蝗存活和生殖的影响 [J]. 应用昆虫学报, 2017, 54(3):426−433.

    XIANG M, FAN T S, HU H X, et al. Effects of short-term exposure to high temperature on the survival and fecundity of Calliptamus italicus(Orthopera: Acrididae) [J]. Chinese Journal of Applied Entomology, 2017, 54(3): 426−433.(in Chinese)
    [32] 蒋明星, 张孝羲, 李永飞. 日短期高温对棉铃虫实验种群的影响 [J]. 昆虫知识, 1998, 35(3):139−141.

    JIANG M X, ZHANG X X, LI Y F. Effects of short-term high temperature on experimental population of cotton bollworm [J]. Entomological Knowledge, 1998, 35(3): 139−141.(in Chinese)
    [33] 崔旭红, 徐建信, 李晓宇, 等. 短时高温暴露对Q型烟粉虱成虫存活和生殖适应性的影响 [J]. 中国农学通报, 2011, 27(5):377−379.

    CUI X H, XU J X, LI X Y, et al. Effects of brief exposure to high temperature on survival and fecundity of Bemisia tabaci Q-biotype (Homoptera: Aleyrodidae) [J]. Chinese Agricultural Science Bulletin, 2011, 27(5): 377−379.(in Chinese)
    [34] 蒋丰泽, 郑灵燕, 郭技星, 等. 温度对昆虫繁殖力的影响及其生理生化机制 [J]. 环境昆虫学报, 2015, 37(3):653−663.

    JIANG F Z, ZHENG L Y, GUO J X, et al. Effects of temperature stress on insect fertility and its physiological and biochemical mechanisms [J]. Journal of Environmental Entomology, 2015, 37(3): 653−663.(in Chinese)
    [35] 韩永旭. 高温对亚洲玉米螟生长发育和繁殖的影响[D]. 沈阳: 沈阳农业大学, 2017.

    HAN Y X. Effects of high temperature on development and fecundity of Ostrinia furnacalis(Guenée)[D]. Shenyang: Shenyang Agricultural University, 2017. (in Chinese)
    [36] 杨步莲. 高温对抗性和敏感小菜蛾体内保护酶系的影响[D]. 福州: 福建农林大学, 2012.

    YANG B L. Effects of high temperature on protective enzyme in resistant and susceptible Plutella xylostella[D]. Fuzhou: Fujian Agriculture and Forestry University, 2012. (in Chinese)
    [37] 李爽. 雌雄意大利蝗耐高温差异及其生理生化响应对策研究[D]. 乌鲁木齐: 新疆师范大学, 2016.

    LI S. Differences in heat tolerance and physio-biochemical mechanisms between female and male Calliptamus italicus adults (Orthopera: Acrididae)[D]. Urumqi: Xinjiang Normal University, 2016. (in Chinese)
    [38] FRIDOVICH I. Free radical in biology[M]. New York: Academic press, 1976.
    [39] PACKER L, OTTING F. Oxygen radicals in biological systems [J]. Academic Press, 1984, 105(1): 273−280.
    [40] FRIDOVICH I. Oxygen is toxic! [J]. BioScience, 1977, 27(7): 462−466. doi: 10.2307/1297527
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  • 收稿日期:  2023-06-06
  • 修回日期:  2023-07-31
  • 网络出版日期:  2023-12-21
  • 刊出日期:  2023-11-28

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