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甜玉米自交系重金属Pb、Cd积累差异及分子检测

李淑君 刘娅娟 张扬 蒲汝民 袁亮 廖长见

李淑君,刘娅娟,张扬,等. 甜玉米自交系重金属Pb、Cd积累差异及分子检测 [J]. 福建农业学报,2024,39(3):251−258 doi: 10.19303/j.issn.1008-0384.2024.03.002
引用本文: 李淑君,刘娅娟,张扬,等. 甜玉米自交系重金属Pb、Cd积累差异及分子检测 [J]. 福建农业学报,2024,39(3):251−258 doi: 10.19303/j.issn.1008-0384.2024.03.002
LI S J, LIU Y J, ZHANG Y, et al. Accumulation and Molecular Detection of Pb and Cd in Sweet Corn Inbred Lines [J]. Fujian Journal of Agricultural Sciences,2024,39(3):251−258 doi: 10.19303/j.issn.1008-0384.2024.03.002
Citation: LI S J, LIU Y J, ZHANG Y, et al. Accumulation and Molecular Detection of Pb and Cd in Sweet Corn Inbred Lines [J]. Fujian Journal of Agricultural Sciences,2024,39(3):251−258 doi: 10.19303/j.issn.1008-0384.2024.03.002

甜玉米自交系重金属Pb、Cd积累差异及分子检测

doi: 10.19303/j.issn.1008-0384.2024.03.002
基金项目: 重庆市自然科学基金面上项目(cstc2021jcyj-msxmX0226);福建省农业科学院自由探索项目(ZYTS2023004 )
详细信息
    作者简介:

    李淑君(1986 —),女,助理研究员,主要从事玉米遗传育种研究,E-mail:272245217@qq.com

    通讯作者:

    廖长见(1979 —),男,副研究员,主要从事玉米遗传育种研究,E-mail:liaocj1978@163.com

  • 中图分类号: S513

Accumulation and Molecular Detection of Pb and Cd in Sweet Corn Inbred Lines

  • 摘要:   目的  筛选获得在重金属Pb、Cd单一污染及复合污染下Pb、Cd低积累的优良甜玉米自交系,以及可用于甜玉米重金属Pb、Cd积累早代鉴定的分子技术。  方法  以10个甜玉米自交系为供试材料,通过盆栽试验研究重金属Pb、Cd单一污染及Pb、Cd复合污染下,玉米根系、茎叶和籽粒中Pb、Cd含量的积累差异;同时对供试材料ZmHMA2 InDel位点进行Pb、Cd积累差异分子检测,综合分子标记检测与重金属Pb、Cd在不同材料间的积累结果,筛选甜玉米重金属低积累自交系。  结果  无论是单一污染还是复合污染,重金属Pb、Cd的积累规律均表现为根系>茎叶>籽粒;在复合污染下,玉米不同组织对重金属Pb、Cd的积累无明显竞争与协同效应,表明玉米Pb、Cd的积累机制存在一定差异。通过鉴定,获得籽粒Pb低积累玉米自交系2份,籽粒Cd低积累玉米自交系3份,仅有一份材料闽甜系X901表现出籽粒Pb、Cd均低积累。利用InDel 位点(InDel2307)对供试材料进行分子检测,结果发现供试材料中有4份存在该位点,含有该位点的材料中根系、茎叶和籽粒Cd含量平均值较其他材料平均值分别低1.801、0.64 、0.131 mg·kg−1  结论  InDel2307位点能将不同玉米自交系按Cd累积量进行区分,对甜玉米Cd含量的区分具有特异性标记。综合分子标记检测与Pb、Cd含量积累结果,筛选出自交系闽甜系X901 为Pb、Cd低积累材料。
  • 图  1  10个玉米自交系Indel2307位点的序列比对

    Figure  1.  Sequence alignment of Indel 2307 locus in 10 sweet corn inbred lines

    表  1  供试甜玉米材料编号及名称

    Table  1.   Codes and names of sweet corn inbred lines

    编号
    No.
    自交系
    Inbred line
    编号
    No.
    自交系
    Inbred line
    S1 闽甜系688 S6 闽甜系AS74
    S2 闽甜系G23 S7 闽甜系AS76
    S3 闽甜系H6 S8 闽甜系T146
    S4 闽甜系197 S9 闽甜系X901
    S5 闽甜系AS67 S10 闽甜系JR8609
    下载: 导出CSV

    表  2  ZmHMA2基因所用引物

    Table  2.   Primers used in amplifying ZmHMA2

    引物名称
    Primer name
    正向引物 (5′-3′)
    Primer sequence(5′-3′)
    反向引物(5′-3′)
    Primer sequence(5′-3′)
    ZmHMA2-1 TTATCGCTCCGGATATGCCC CCATCCATCCCTTCAGCCTTT
    ZmHMA2-2 GGATATGCCCTCTCCAGGGT CCATCCATCCCTTCAGCCTT
    ZmHMA2-3 TTTATCGCTCCGGATATGCCC CATCCATCCCTTCAGCCTTT
    下载: 导出CSV

    表  3  单一Pb污染环境下玉米不同基因型及不同部位重金属Pb含量

    Table  3.   Pb contents in tissues of sweet corn inbred lines grown under Pb-contaminated soil

    自交系
    Inbred line
    Pb含量 Pb content/(mg·kg−1 分子检测
    Marker detection
    根 Roots 茎叶 Shoots 籽粒 Grains
    闽甜系688(S1) 240.171±1.023 bcA 89.531±1.101 cB 0.312±0.019 bC
    闽甜系G23(S2) 214.412±1.201 dA 87.408±1.062 cB 0.244±0.018 cC
    闽甜系H6(S3) 176.303±2.390 efA 86.721±1.112 cB 0.135±0.018 eC
    闽甜系197(S4) 233.591±2.331 cA 87.295±0.945 cB 0.223±0.009 cC +
    闽甜系AS67(S5) 259.374±3.031 aA 125.216±2.213 aB 0.381±0.010 aC +
    闽甜系AS74(S6) 249.845±1.944 bA 120.911±1.409 abB 0.321±0.011 a C +
    闽甜系AS76(S7) 248.210±2.802 bA 90.923±1.204 cB 0.227±0.017 cC
    闽甜系T146(S8) 260.183±1.255 aA 114.475±1.301 bB 0.397±0.018 aC
    闽甜系X901(S9) 169.003±3.501 fA 89.782±0.928 cB 0.172±0.010 dC +
    闽甜系JR8609(S10) 185.784±2.902 eA 91.761±2.691 cB 0.344±0.010 bC
    同行数据后不同大写字母表示不同组织部位间差异极显著(P<0.01),同列数据后不同小写字母表示不同自交系间差异显著(P<0.05)。下表同。
    Data with different capital letters on same row indicate extremely significant differences among different tissues at P<0.01; those with different lowercase letters on the same column indicate significant differences among different inbred line at P<0.05. Same for below.
    下载: 导出CSV

    表  4  单一Cd污染环境下玉米不同基因型及不同部位重金属Cd含量

    Table  4.   Cd contents in tissues of sweet corn inbred lines grown under Cd-contaminated soil

    编号
    No.
    Cd含量 Cd content/(mg·kg−1
    根系 Roots 茎叶 Shoots 籽粒 Grains
    闽甜系688(S1) 3.528±0.102 aA 1.723±0.116 abB 0.213±0.121 bC
    闽甜系G23(S2) 3.823±0.123 aA 1.285±0.126 bcB 0.181±0.120 cC
    闽甜系H6(S3) 3.124±0.022 bA 1.992±0.092 aB 0.241±0.018 aC
    闽甜系197(S4) 1.055±0.134 eA 1.137±0.201 cB 0.106±0.015 dC
    闽甜系AS67(S5) 1.231±0.094 eA 0.988±0.132 dB 0.086±0.021 eC
    闽甜系AS74(S6) 1.166±0.236 eA 0.932±0.144 dB 0.091±0.012 deC
    闽甜系AS76(S7) 2.931±0.136 cA 2.022±0.118 aB 0.234±0.011 aC
    闽甜系T146(S8) 2.215±0.254 cdA 1.535±0.087 bB 0.209±0.011 bC
    闽甜系X901(S9) 1.043±0.108 eA 0.772±0.102 eB 0.063±0.001 fC
    闽甜系JR8609(S10) 1.929±0.203 dA 1.025±0.121 dB 0.225±0.012 abC
    下载: 导出CSV

    表  5  Pb-Cd复合污染下玉米不同基因型及不同部位重金属Pb、Cd含量

    Table  5.   Pb and Cd contents in tissues of sweet corn inbred lines grown under Pb-and-Cd-contaminated soil

    编号
    No.
    根系重金属含量
    Heavy metal content in roots/(mg·kg−1)
    茎叶重金属含量
    Heavy metal content in shoots/(mg·kg−1)
    籽粒重金属含量
    Heavy metal content in grains/(mg·kg−1)
    Pb Cd Pb Cd Pb Cd
    闽甜系688(S1) 225.182±2.211 c 2.981±0.130 a 95.427±2.122 b 1.231±0.122 c 0.432±0.022 ab 0.360±0.020 a
    闽甜系G23(S2) 218.523±4.652 d 2.594±0.221 b 94.143±1.842 b 0.924±0.114 c 0.392±0.021 b 0.141±0.021 c
    闽甜系H6(S3) 167.623±3.692 f 3.049±0.182 a 86.613±1.553 c 1.771±0.191 a 0.083±0.038 e 0.350±0.025 a
    闽甜系197(S4) 219.825±2.622 d 1.911±0.372 c 95.310±1.190 b 0.908±0.127 d 0.309±0.011 c 0.091±0.024 d
    闽甜系AS67(S5) 234.535±4.741 b 1.263±0.218 d 115.542±1.424 a 0.815±0.102 d 0.429±0.017 ab 0.087±0.019 d
    闽甜系AS74(S6) 228.191±3.505 c 2.406±0.132 b 119.261±1.211 a 1.197±0.119 c 0.471±0.022 a 0.102±0.019 c
    闽甜系AS76(S7) 228.435±2.142 c 3.044±0.159 a 79.243±2.107 d 1.551±0.149 b 0.383±0.033 b 0.178±0.022 b
    闽甜系T146(S8) 246.653±2.712 a 1.951±0.154 c 111.020±1.508 a 1.168±0.214 c 0.463±0.018 a 0.155±0.024 bc
    闽甜系X901(S9) 159.371±3.443 f 1.221±0.213 d 93.035±1.416 b 0.536±0.275 e 0.094±0.018 e 0.074±0.020 d
    闽甜系JR8609(S10) 196.029±1.961 e 1.408±0.126 d 85.861±1.312 c 0.860±0.112 d 0.245±0.032 d 0.151±0.021 c
    下载: 导出CSV
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出版历程
  • 收稿日期:  2023-11-22
  • 修回日期:  2024-02-05
  • 网络出版日期:  2024-05-08
  • 刊出日期:  2024-03-28

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