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复合微生物菌剂对毛竹土壤细菌群落结构的影响

袁宗胜

袁宗胜. 复合微生物菌剂对毛竹土壤细菌群落结构的影响 [J]. 福建农业学报,2024,39(4):438−447 doi: 10.19303/j.issn.1008-0384.2024.04.008
引用本文: 袁宗胜. 复合微生物菌剂对毛竹土壤细菌群落结构的影响 [J]. 福建农业学报,2024,39(4):438−447 doi: 10.19303/j.issn.1008-0384.2024.04.008
YUAN Z S. Soil Microbiome at Phyllostachys edulis Forest Affected by Application of Bioagent [J]. Fujian Journal of Agricultural Sciences,2024,39(4):438−447 doi: 10.19303/j.issn.1008-0384.2024.04.008
Citation: YUAN Z S. Soil Microbiome at Phyllostachys edulis Forest Affected by Application of Bioagent [J]. Fujian Journal of Agricultural Sciences,2024,39(4):438−447 doi: 10.19303/j.issn.1008-0384.2024.04.008

复合微生物菌剂对毛竹土壤细菌群落结构的影响

doi: 10.19303/j.issn.1008-0384.2024.04.008
基金项目: 福建省林业科技研究项目(2021FKJ07)
详细信息
    作者简介:

    袁宗胜(1976 —),男,博士,副教授,主要从事森林资源培育、微生物等方面研究,E-mail:yuanzs369@163.com

  • 中图分类号: Q938

Soil Microbiome at Phyllostachys edulis Forest Affected by Application of Bioagent

  • 摘要:   目的  探究复合微生物菌剂[产气肠杆菌(Enterobacter aerogenes)CT-B09-2、解淀粉芽孢杆菌(Bacillus amyloliquefaciens)JL-B06和乙酸钙不动杆菌(Acinetobacter calcoaceticus)WYS-A01-1]对毛竹土壤细菌群落结构和多样性的影响。  方法  以毛竹(Phyllostachys edulis)实生幼苗为研究对象,采用灌根的方式施用复合微生物菌剂,30 d后采集毛竹幼苗根际与非根际土壤样本,测定土壤理化性质,提取土壤总DNA并进行16S rRNA高通量测序,分析复合微生物菌剂对土壤细菌群落结构和多样性的影响。  结果  复合微生物菌剂可以有效提升土壤中速效磷的含量,调控土壤pH,提高根际土壤中物质代谢和碳化合物分解相关的功能活性。毛竹根际与非根际土壤共检测出26门、65纲、158目、253科、448属、674种微生物,主要优势菌门包括变形菌门 Proteobacteria、拟杆菌门 Firmicutes、放线菌门 Actinobacteriota、酸杆菌门 Acidobacteriota、绿弯菌门 Chloroflexi等。施用复合微生物菌剂后,毛竹根际土壤微生物群落物种数目显著上升,非根际土壤样本无显著变化。  结论  复合微生物菌剂可以调节土壤矿质元素,改善土壤pH,调控细菌微生物群落组成。
  • 图  1  样本稀释曲线和Venn图

    A:稀释曲线;B:(a)复合微生物菌剂施用的根际土壤ASV对比;(b)复合微生物菌剂施用的非根际土壤ASV对比;(c)样本总体ASV对比。

    Figure  1.  Sample dilution curve and Venn diagram

    A: dilution curve; B: (a) ASVs of rhizosphere soils treated with bioagent; (b) ASVs of non-rhizosphere soils treated with bioagent; (c) ASVs of total sample population.

    图  2  NMDS分析

    Figure  2.  NMDS analysis

    图  3  门水平下的物种相对丰度

    A:相对丰度;B:样本间显著差异箱图。图4同。

    Figure  3.  Relative species abundance at phylum level

    A: histogram of relative abundance; B: box chart of significant differences between samples. Same for Fig.4.

    图  4  属水平下的物种相对丰度

    Figure  4.  Relative abundance of species at genus level

    图  5  样本环境因子与门水平物种RDA分析

    Figure  5.  RDA analysis on environmental factors and species at phylum level

    图  6  土壤细菌功能预测分析

    Figure  6.  Predicted functions of soil microbes

    表  1  样本编号对照表

    Table  1.   Sample codes

    样本类型
    Sample type
    分组
    Group
    对照组毛竹根际土壤 E0
    对照组毛竹非根际土壤 F0
    复合微生物菌剂处理组毛竹根际土壤 E1
    复合微生物菌剂处理组毛竹非根际土壤 F1
    下载: 导出CSV

    表  2  土壤理化性质测定结果

    Table  2.   Physiochemical properties of soil samples

    样本
    Sample
    有机质
    OM/ (g·kg−1)
    速效磷
    AP/(μmol·g−1
    速效钾
    AK/(mg·kg−1
    全钾
    TK/(g·kg−1
    全氮
    TN/(g·kg−1
    全磷
    TP/(mg·kg−1
    速效氮
    AN/(mg·kg−1
    pH
    E0 20.81±0.01a 4.42±0.02c 329.12±0.02b 14.79±0.02b 0.97±0.02a 507.04±0.02b 86.34±0.01a 5.68±0.01d
    E1 7.67±0.01c 4.82±0.03a 196.71±0.01d 13.75±0.02c 0.75±0.03b 480.41±0.03c 43.03±0.02c 5.88±0.01c
    F0 17.84±0.01b 4.50±0.01b 341.53±0.03a 15.84±0.04a 0.99±0.01a 531.07±0.04a 68.96±0.04b 6.14±0.02b
    F1 7.01±0.01d 4.51±0.01b 261.03±0.04c 13.73±0.03c 0.77±0.02b 488.1±0.02c 47.05±0.03c 6.54±0.02a
    图中数据为平均值±标准差;同列数据后不同小写字母表示不同处理间差异显著(P < 0.05)。表3同。
    Data are mean±standard deviation; those with different lowercase letters indicate significant differences between treatments at P<0.05. Same for Table 3.
    下载: 导出CSV

    表  3  Alpha 多样性指数

    Table  3.   Alpha diversity index

    样本
    Sample
    丰度指数
    ACE
    查尔指数
    Chao1
    香农指数
    Shannon
    辛普森指数
    Simpson
    E0 479.84±32.70b 479.76±32.55b 5.74±0.11a 0.0048±0.001b
    E1 539.52±80.66a 539.20±80.37a 5.76±0.06a 0.0055±0.001b
    F0 493.95±96.69b 493.66±96.37b 5.67±0.19a 0.0054±0.001b
    F1 489.88±127.07b 489.25±126.73b 5.56±0.29a 0.0079±0.004a
    下载: 导出CSV

    表  4  细菌功能表达丰度

    Table  4.   Significant differences in microbial functions

    功能 Function E0 E1 F0 F1
    光养 Phototrophy 1.54±0.37c 1.61±0.09b 2.62±0.26a 2.32±0.26a
    光合自养 Photoautotrophy 1.47±0.38d 1.61±0.09c 2.60±0.29a 2.26±0.32b
    蓝藻 Cyanobacteria 1.23±0.36d 1.55±0.04c 2.56±0.35a 2.26±0.32b
    含氧光能自养 Oxygenic photoautotrophy 1.23±0.36d 1.55±0.04c 2.56±0.35a 2.26±0.32b
    固氮作用 Nitrogen fixation 3.98±1.90a 1.94±0.20b 0.75±0.31c 0.67±0.36c
    硝酸盐呼吸 Nitrate respiration 0.98±0.58a 0.26±0.12c 0.84±0.32b 0.20±0.09c
    氮呼吸 Nitrogen respiration 0.98±0.58a 0.26±0.12b 0.84±0.32a 0.20±0.09b
    纤维素分解 Cellulolysis 1.04±0.50a 0.50±0.32b 0.16±0.02c 0.15±0.16c
    甲基营养 Methylotrophy 0.19±0.01a 0.05±0.08c 0.19±0.02a 0.08±0.05b
    甲醇氧化 Methanol oxidation 0.19±0.01a 0.05±0.08c 0.19±0.02a 0.08±0.05b
    铁呼吸 Iron respiration 0d 0.02±0.03c 0.14±0.04a 0.07±0.07b
    芳香烃碳氢降解 Aromatic hydrocarbon degradation 0c 0.02±0.02b 0c 0.09±0.07a
    脂族非甲烷碳氢化合物降解
    Aliphatic non methane hydrocarbon degradation
    0c 0.02±0.02b 0c 0.09±0.07a
    碳氢化合物降解 Hydrocarbon degradation 0c 0.02±0.02b 0c 0.09±0.07a
    下载: 导出CSV
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  • 收稿日期:  2023-08-17
  • 修回日期:  2023-11-13
  • 网络出版日期:  2024-06-26
  • 刊出日期:  2024-04-28

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