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PGPR微胶囊菌剂研发及其对玉米的促生效果评价

刘文佳 刘天一 张俐敏 徐畅 莫继先

刘文佳,刘天一,张俐敏,等. PGPR微胶囊菌剂研发及其对玉米的促生效果评价 [J]. 福建农业学报,2024,39(X):1−11
引用本文: 刘文佳,刘天一,张俐敏,等. PGPR微胶囊菌剂研发及其对玉米的促生效果评价 [J]. 福建农业学报,2024,39(X):1−11
LIU W J, LIU T Y, ZHANG L M, et al. Development of microencapsulated rhizosphere growth promoting bacteria and evaluation of the growth promoting effects on corn [J]. Fujian Journal of Agricultural Sciences,2024,39(X):1−11
Citation: LIU W J, LIU T Y, ZHANG L M, et al. Development of microencapsulated rhizosphere growth promoting bacteria and evaluation of the growth promoting effects on corn [J]. Fujian Journal of Agricultural Sciences,2024,39(X):1−11

PGPR微胶囊菌剂研发及其对玉米的促生效果评价

基金项目: 黑龙江省省属高等学校基本科研业务费项目(135409420);黑龙江省大学生创新创业训练计划项目(S202310232017)
详细信息
    作者简介:

    刘文佳(1999 —),女,硕士研究生,研究方向:资源环境微生物学(E-mail:1740203063@qq.com

    通讯作者:

    莫继先(1982 —),男,博士,教授,研究方向:资源环境微生物学(E-mail:mojixian8208@sina.com

  • 中图分类号: S541

Development of microencapsulated rhizosphere growth promoting bacteria and evaluation of the growth promoting effects on corn

  • 摘要:   目的  为了提高PGPR对作物促生作用的稳定性,研制出以PGPR复合菌液为原料的微胶囊菌剂,探究其对玉米的促生效果。  方法  从玉米根际土壤中分离得到2株PGPR菌株并制备成复合液体菌液。分别用海藻酸钠(SA)和CaCl2作为包埋剂和交联剂,对复合菌液进行包埋以制备微胶囊菌剂。以复合微胶囊菌剂制备上的难易程度和菌株包埋率、增殖前活菌数及增殖后活菌数为评价指标,确定微胶囊菌剂制备的最优条件。通过分析微胶囊菌剂在不同聚乙二醇浓度、温度、pH、存储时间以及盐浓度下的菌株生长及其对玉米生长和根系形态的影响确定PGPR微胶囊菌剂的特性。  结果  研究分离得到了2株PGPR菌株,具有多种植物促生功能。使用1%SA-3%CaCl2制备的微胶囊菌剂稳定性较好,包埋率达到91.27%,增殖后的微胶囊活菌数达到8.73×109 cfu·g−1,增殖7.79倍。微胶囊菌剂在不同干旱、温度、pH、存储时间和盐浓度条件下对PGPR菌株具有较好的保护作用。PGPR微胶囊菌剂对玉米有显著的促生作用,施用微胶囊菌剂使玉米株高、苗干重和根干重分别增加91.83%、81.82%和29.57%。同时微胶囊菌剂也显著提高了玉米总根长、根表面积、根平均直径、根体积和根尖数。  结论  确定了一种PGPR微胶囊菌剂的制备方法,施用该PGPR微胶囊剂能够显著促进玉米生长、改善玉米根系形态,对玉米的促生效果优于液体菌剂。
  • 图  1  菌株P1和K5的菌间拮抗实验

    Figure  1.  Interbacterial antagonism assay of strains P1 and K5

    图  2  菌株 P1(a)和K5(b)的显微形态图

    Figure  2.  Microscopic morphology of P1 (a) and K5 (b) strains

    图  3  菌株P1的系统进化树

    Figure  3.  Phylogenetic tree of strain P1

    图  4  菌株K5的系统进化树

    Figure  4.  Phylogenetic tree of strain K5

    图  5  菌株P1和K5的生长曲线

    Figure  5.  Growth curves of strains P1 and K5

    图  6  SA1%-CaCl23%微胶囊颗粒的形态图

    Figure  6.  Morphological diagram of SA1%-CaCl23% microcapsule particles

    图  7  不同环境条件对两种剂型菌株生长的影响

    *表示两组之间差异显著(P<0.05)

    Figure  7.  Effects of different environmental conditions on the growth of two dosage form strains

    *indicated a significant difference between the two groups (P<0.05)

    图  8  不同剂型PGPR对玉米生长的影响

    a:各处理组对玉米株高的影响;b:各处理组对玉米苗干重的影响;c:各处理组对玉米根干重的影响;不同小写字母表示不同组别间差异显著(P<0.05)

    Figure  8.  Effects of different dosage forms of PGPR on the growth of corn

    a: The effects of each treatment group on the plant height of corn; b: The effects of each treatment group on the dry weight of corn seedlings; c: The effects of each treatment group on the dry weight of corn roots; Different letters indicated significant differences between different groups (P<0.05)

    图  9  不同剂型PGPR对玉米根系形态的影响

    a:各处理组对玉米根长的影响;b:各处理组对玉米根总表面积的影响;c:各处理组对玉米根平均直径的影响;d:各处理组对玉米根尖数的影响;e:各处理组对玉米根体积的影响;不同字母表示不同组别间差异显著(P<0.05)

    Figure  9.  Effects of different dosage forms of PGPR on corn root morphology

    a: The effects of each treatment group on the corn root length; b: The effects of each treatment group on the total surface area of corn roots; c: The effects of each treatment group on the average diameter of corn roots; d: The effects of each treatment group on the number of corn root tips; e: The effects of each treatment group on the volume of corn roots; Different letters indicated significant differences between different groups (P<0.05)

    表  1  玉米根际土壤中筛选的具有促生特性的菌株及促生特性分析

    Table  1.   Analysis of strains with growth-promoting characteristics and growth-promoting characteristics screened in corn rhizosphere soil

    菌株
    Strains
    解磷量
    Phosphate solubilizing/
    (μg·mL−1)
    解钾量
    Potassium releasing/
    (μg·mL−1)
    固氮量
    Nitrogen fixation/
    (μg·mL−1)
    吲哚乙酸
    Indoleacetic acid/
    (μg·mL−1)
    铁载体活性
    Siderophore activity/(%)
    ACC含量
    ACC content/
    (μg·mL−1)
    P1 33.50±0.37a 1.44±0.16b 78.06±0.51a 55.72±0.67a 0.19±0.07b
    P2 31.49±0.51b 65.74±1.61b 44.19±0.94d 0.30±0.07e
    P3 31.34±0.58b 49.12±0.35b 0.27±0.05d
    P4 30.85±0.59b 46.47±0.27c 0.22±0.14c
    P5 30.12±0.03c 46.23±3.25d 40.48±0.44e 0.14±0.07a
    P6 31.13±0.04b 39.65±0.38e 0.20±0.06b
    N1 1.96±0.35a 33.5±0.527f 0.27±0.11d
    N2 1.27±0.21b 0.14±0.02a
    N3 1.23±0.10b 0.22±0.03c
    K1 1.22±0.09b 55.63±0.54c 31.52±0.39g 0.14±0.02a
    K2 1.17±0.07b 0.30±0.06e
    K3 1.26±0.07b 29.34±0.35g 0.25±0.13cd
    K4 1.35±0.28b 0.18±0.07b
    K5 2.26±0.07a 63.43±2.9b 50.46±0.71b 0.34±0.05f
    同列不同行有不同字母表示差异显著(P<0.05);—表示无此项促生功能
    Different letters in different rows indicated significant differences (P<0.05); —indicated no growth-promoting function
    下载: 导出CSV

    表  2  菌株P1和K5的生理生化特性

    Table  2.   Physiological and biochemical characteristics of strains P1 and K5

    实验项目
    Items
    菌株 Strains
    P1 K5
    淀粉水解实验
    Starch hydrolysis
    蔗糖发酵实验
    Sucrose fermentation
    +
    乳糖发酵实验
    Lactose fermentation
    +
    葡萄糖发酵实验
    Glucose fermentation
    +
    甲基红实验
    Methyl red experiment
    柠檬酸盐实验
    Citrate experiment
    吲哚实验
    Indole experiment
    + +
    革兰氏染色实验
    Gram staining experiment
    + +
    触酶实验
    Catalase experiment
    + +
    伏-普实验
    Volt-P experiment
    +
    +:阳性;−:阴性;
    +: positive; −: negative
    下载: 导出CSV

    表  3  SA-CaCl2不同浓度配比下微胶囊的操作性和成球性

    Table  3.   Operability and spherical properties of microcapsules under different concentration ratios of SA-CaCl2

    SA浓度/%
    Concentration
    of SA/
    (%)
    CaCl2浓度/%
    Concentration
    of CaCl2/
    (%)
    操作性
    Operation
    成球性
    Sphericity
    1 2
    Easy
    较好,拖尾,无粘连
    Better, trailing, no sticking
    1 3
    Easy
    较好,拖尾,少量粘连
    Better, trailing, few sticks
    1 4
    Easy
    较好,拖尾,少量粘连
    Better, trailing, few sticks
    2 2
    Easy
    较好,拖尾,无粘连
    Better, trailing, no sticking
    2 3
    Easy
    较好,拖尾,少量粘连
    Better, trailing, few sticks
    2 4
    Easy
    较好,拖尾,少量粘连
    Better, trailing, few sticks
    3 2
    Difficult
    较差,无拖尾,少量破碎
    Bad, no tailing, few fragile
    3 3
    Difficult
    较差,拖尾,少量粘连
    Bad, trailing, few sticks
    3 4
    Difficult
    差,拖尾,少量破碎,易粘连
    Poor, trailing, few fragile, sticks
    下载: 导出CSV

    表  4  微胶囊菌剂颗粒的理化性质

    Table  4.   Physicochemical properties of microcapsule particles

    指标
    Index
    SA1%-CaCl22% SA1%-CaCl23% SA2%-CaCl22%
    直径(mm)
    Diameter (mm)
    3.14±0.13b 3.11±0.07c 3.22±0.04a
    机械强度(g)
    Mechanical Strength (g)
    3.29±0.16b 3.21±0.13c 3.35±0.03a
    增殖前胶囊活菌数(109 cfu·g−1
    Number of viable cells before proliferation (109 cfu·g−1)
    1.01±0.11b 1.12±0.03c 1.20±0.03a
    包埋率(%)
    Embedding rate (%)
    85.60±2.25b 91.27±1.05c 81.17±1.91a
    增殖后胶囊活菌数(109 cfu·g−1
    Number of viable cells in after proliferation (109 cfu·g−1)
    5.68±0.37b 8.73±0.50c 6.91±0.49a
    增殖倍数
    Multiplication ratio
    5.62 7.79 5.76
    同行不同列有不同字母表示差异显著(P<0.05)
    Different letters in the different column indicated significant differences (P<0.05)
    下载: 导出CSV
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出版历程
  • 收稿日期:  2023-12-22
  • 修回日期:  2024-02-07
  • 网络出版日期:  2024-03-28

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