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SOCS3负调控流感病毒诱导干扰素表达机理的初步研究

彭本群 胡精蕴 毛亚楠 王淑霖 王佳俊 陈梦颖 尤冬雪 王松

彭本群,胡精蕴,毛亚楠,等. SOCS3负调控流感病毒诱导干扰素表达机理的初步研究 [J]. 福建农业学报,2024,39(2):131−136 doi: 10.19303/j.issn.1008-0384.2024.02.002
引用本文: 彭本群,胡精蕴,毛亚楠,等. SOCS3负调控流感病毒诱导干扰素表达机理的初步研究 [J]. 福建农业学报,2024,39(2):131−136 doi: 10.19303/j.issn.1008-0384.2024.02.002
PENG B Q, HU J Y, MAO Y N, et al. A Preliminary Study on Influenza Virus-induced Interferon Regulating Mechanism of SOCS3 [J]. Fujian Journal of Agricultural Sciences,2024,39(2):131−136 doi: 10.19303/j.issn.1008-0384.2024.02.002
Citation: PENG B Q, HU J Y, MAO Y N, et al. A Preliminary Study on Influenza Virus-induced Interferon Regulating Mechanism of SOCS3 [J]. Fujian Journal of Agricultural Sciences,2024,39(2):131−136 doi: 10.19303/j.issn.1008-0384.2024.02.002

SOCS3负调控流感病毒诱导干扰素表达机理的初步研究

doi: 10.19303/j.issn.1008-0384.2024.02.002
基金项目: 国家自然科学基金项目(U23A20235);福建省自然科学基金项目(2020J06016);福建农林大学科技创新专项基金(KFb22063XA)
详细信息
    作者简介:

    彭本群(1999 —),男,硕士研究生, 主要从事预防兽医学研究,E-mail:benjamin_Pong@outlook.com

    通讯作者:

    王松(1983 —),男,博士,教授,主要从事动物病原微生物与免疫学研究,E-mail:wscookie@163.com

  • 中图分类号: S852.65

A Preliminary Study on Influenza Virus-induced Interferon Regulating Mechanism of SOCS3

  • 摘要:   目的  探究细胞因子信号转导抑制因子3(Suppressor of cytokine signaling 3, SOCS3)在流感病毒(Influenza virus)感染过程中对干扰素(Interferon, IFN)信号通路的调控作用。  方法  通过慢病毒感染的方式在人非小细胞肺癌细胞(A549细胞)中过表达SOCS3,利用siRNA技术在A549细胞中敲低SOCS3的表达,然后使用流感病毒感染SOCS3过表达或敲低的细胞系以及对照细胞系,于不同时间点收取RNA或蛋白样品,采用RT-PCR和Western blot技术检测干扰素信号通路中关键节点分子的表达或活化情况。  结果  检测发现细胞中过表达SOCS3后I型干扰素IFN-β和III型干扰素IL-28(Interleukin-28)、IL-29(Interleukin-29)表达水平下降,而敲低SOCS3表达后IFN-βIL-28IL-29的表达水平升高。进一步研究发现,过表达SOCS3对识别流感病毒RNA的模式识别受体视黄酸诱导基因-I(Retinoic acid-inducible gene 1, RIG-I)、黑色素瘤分化相关蛋白5(Melanoma differentiation-associated protein 5, MDA5)、Toll样受体3(Toll-like receptor 3, TLR3)以及干扰素调节因子7(Interferon regulatory factor 7, IRF7)的mRNA表达均有抑制作用。同时SOCS3还能影响干扰素下游信号转导与转录激活因子 1(Signal transducer and activator of transcription 1, STAT1)的活化,细胞中过表达SOCS3抑制流感病毒诱导的STAT1的磷酸化,而敲低SOCS3表达则使STAT1的磷酸化水平升高。  结论  流感病毒感染后,SOCS3能够在转录水平下调模式识别受体及干扰素调节因子的表达,抑制Ⅰ型和Ⅲ型干扰素的产生,同时还影响STAT1的活化从而阻断干扰素信号的传递。
  • 图  1  SOCS3抑制流感病毒感染后干扰素的产生

    Figure  1.  Inhibition of interferon production by SOCS3 after influenza virus infection

    图  2  SOCS3抑制模式识别受体的表达

    Figure  2.  Inhibition of pattern recognition receptor expressions by SOCS3

    图  3  SOCS3抑制IRF7的表达

    Figure  3.  Inhibition of IRF7 expression by SOCS3

    图  4  SOCS3抑制流感病毒诱导的STAT1磷酸化

    Figure  4.  Inhibition of influenza virus-induced STAT1 phosphorylation by SOCS3

    表  1  RT-PCR引物序列

    Table  1.   Sequences of primers for RT-PCR

    引物
    Primer
    上游引物 5′-3′
    Forward primer 5′-3′
    下游引物 5′-3′
    Reverse primer 5′-3′
    GAPDH TGGGTGTGAACCATGAGAAGT AAGGCCATGCCAGTGAGCTT
    NP (流感病毒) TCAAACGTGGGATCAATG GTGCAGACCGTGCTAAAA
    SOCS3 CAGTACGATGCCCCGCTTTA GAAGCTGGAGACTCAGGTGG
    IFN-β GCACTGGCTGGAATGAGACTA ACTCTGACTATGGTCCAGGCA
    IL-28 CCTGGTGGACGTCTTGGAC GCGGAAGAGGTTGAAGGTGA
    IL-29 TGGTGACTTTGGTGCTAGGC GGCCTTCTTGAAGCTCGCTA
    RIG-I GAAGATCCAGAATGCCAGAATC CTGGGATCTGATTCGCAAAA
    MDA5 CTGCTGCAGAAAACAATGGA TGGCTGAACTGTGGTTGAAA
    TLR3 AGTGCCGTCTATTTGCCACA GCATCCCAAAGGGCAAAAGG
    IRF3 GGGGAGTGATGAGCTACGTG CTACAATGAAGGGCCCCAGG
    IRF7 GAGCTGTGCTGGCGAGAAG CTCTCCAGGAGCCTTGGTTG
    下载: 导出CSV
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出版历程
  • 收稿日期:  2024-01-03
  • 修回日期:  2024-01-29
  • 网络出版日期:  2024-03-28
  • 刊出日期:  2024-02-28

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