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盐胁迫对藜麦种子萌发生长的影响及耐盐机理研究

邓焕莹 钱峰 周琬敏 陈雨寒 颜统利 赵燕昊 蒋玉蓉 陆国权

邓焕莹,钱峰,周琬敏,等. 盐胁迫对藜麦种子萌发生长的影响及耐盐机理研究 [J]. 福建农业学报,2023,38(10):1139−1145 doi: 10.19303/j.issn.1008-0384.2023.10.002
引用本文: 邓焕莹,钱峰,周琬敏,等. 盐胁迫对藜麦种子萌发生长的影响及耐盐机理研究 [J]. 福建农业学报,2023,38(10):1139−1145 doi: 10.19303/j.issn.1008-0384.2023.10.002
DENG H Y, QIAN F, ZHOU W M, et al. Growth and Saline-tolerance of Quinoa Seeds and Seedlings under Salt Stress [J]. Fujian Journal of Agricultural Sciences,2023,38(10):1139−1145 doi: 10.19303/j.issn.1008-0384.2023.10.002
Citation: DENG H Y, QIAN F, ZHOU W M, et al. Growth and Saline-tolerance of Quinoa Seeds and Seedlings under Salt Stress [J]. Fujian Journal of Agricultural Sciences,2023,38(10):1139−1145 doi: 10.19303/j.issn.1008-0384.2023.10.002

盐胁迫对藜麦种子萌发生长的影响及耐盐机理研究

doi: 10.19303/j.issn.1008-0384.2023.10.002
基金项目: 浙江省自然科学基金项目(Y21C130006);浙江省重点研发项目(2021C02057);浙江省农业新品种选育重大科技专项子课题(2016C02050-9-9);浙江农林大学学生科研训练项目(202210341235)
详细信息
    作者简介:

    邓焕莹(2000 —),女,硕士研究生,主要从事农艺与种业研究,E-mail:1361819846@qq.com

    通讯作者:

    蒋玉蓉(1974 —),女,博士,副教授,主要从事作物育种与种质创新研究,E-mail:yurongjiang746@126.com

  • 中图分类号: S519

Growth and Saline-tolerance of Quinoa Seeds and Seedlings under Salt Stress

  • 摘要:   目的  探究盐胁迫对藜麦生长发育及生理特性的影响,并研究3种抗逆基因在藜麦中的响应模式。  方法  以藜麦品种Temuco为材料,用不同浓度NaCl溶液(0、200、 450 mmol·L−1)处理藜麦种子和盆栽幼苗,通过测定种子萌发和幼苗生理指标、成熟植株农艺性状、种子营养成分含量,以及SODPODBADH基因的时空表达,明确不同浓度NaCl胁迫对藜麦种子萌发、幼苗生长和种子品质的影响,以及3种抗逆基因对不同盐胁迫的响应。  结果  (1)在高浓度NaCl(450 mmol·L−1)胁迫下藜麦种子发芽和植株生长均受到明显抑制,影响种子N元素的吸收,Na+/K+比率显著上升,超氧化物歧化酶(SOD)和过氧化物酶(POD)的酶活性显著下降,而丙二醛(MDA)含量明显高于对照。低浓度NaCl(200 mmol·L−1)胁迫有助于藜麦生长发育,除发芽指数下降外,种子活力指数和鲜重显著增加,各营养元素含量不受影响且略有上升,诱导SOD和POD酶活性显著增加,MDA含量与对照相比无明显差别。(2)可溶性糖和脯氨酸含量在200 mmol·L−1和450 mmol·L−1两个盐浓度下,与对照相比较均有显著增加,可溶性糖的增幅分别为68.06%和41.67%,脯氨酸含量的增幅分别为237.38%和189.97%。两者均随着盐浓度的增加呈现先上升后下降的趋势。(3)SODPODBADH三个抗逆基因在高浓度盐胁迫下表达量响应模式存在组织特异性和耐盐敏感性差异。BADHSOD基因在根部组织中的表达量高于茎部和叶片,而POD基因则在叶片组织中的表达量最高,根部最低。  结论  高盐胁迫会影响藜麦Temuco种子的营养成分,使其萌发和生长受到抑制;基因的时间动态表达水平表明,在200 mmol·L−1和450 mmol·L−1两个盐浓度下,BADH均较PODSOD对盐胁迫的响应更为迅速和敏感,因此BADH基因的表达量水平和响应速度可作为藜麦耐盐种质筛选的标记之一。本研究对藜麦的耐盐机制的深入和耐盐种质的选育提供了理论依据。
  • 图  1  Temuco种子盐胁迫下Na+/K+变化

    Figure  1.  Changes of Na+/K+ in Temuco seeds under salt stress

    图  2  NaCl处理后幼苗根、茎、叶的BAHD(A)、POD(B)、SOD(C)相对表达水平

    Figure  2.  Relative expressions of BADH (A), POD (B), and SOD (C) in roots, stems, and leaves of seedlings under NaCl treatments

    图  3  200(A)、450(B) mmol·L−1NaCl处理后不同时间对幼苗BADHSODPOD基因表达水平

    Figure  3.  Expressions of BADH, SOD, and POD in seedlings at different times after 200 mmol·L−1 (A) and 450 mmol·L−1 (B) NaCl treatments

    表  1  藜麦3种基因扩增引物

    Table  1.   Amplification primers of 3 quinoa genes

    引物名称
    Name
    正向引物
    Forward primer
    反向引物
    Reverse primer
    用途
    Use
    BADH-1CTGAAGAAATCATCGGTGATATTCTCAAGGAGACTTGTACCATCBADH cDNA片段扩增
    BADH-2GCATTTGAAGAAAGGGTACACTACGCTTGACTCCTCCCBADH基因定量PCR
    POD-1GGATGTGATGCATCAGTACTAGTAGGACAACAGCATCTCTAGCAGCPOD cDNA片段扩增
    POD-2GATTCAACTCCAGGAAACACACAGCATCTCTAGCAGCATAGGPOD基因定量PCR
    SOD-1AACCACTCAATTTTCTGGAAGAAGTATGCATGCTCCCAAACATCSOD cDNA片段扩增
    SOD-2TGATTTGGAGTGGTTTCAACCAATTAGTCAAGGAGGTGGTSOD基因定量PCR
    下载: 导出CSV

    表  2  不同NaCl浓度对藜麦种子Temuco萌发的影响

    Table  2.   Quinoa seed germination affected by NaCl stress

    NaCl浓度
    NaCl concentration/( mmol·L−1)
    发芽率
    Germination rate/%
    发芽势
    Germination potential/%
    发芽指数
    Germination index
    活力指数
    Vitality Index
    株高
    Plant height/cm
    鲜重
    Fresh weight/g
    085.40 a78.72 a16.53 a3.28 b3.87 a0.22 b
    20080.34 a72.52 a11.93 b4.85 a4.21 a0.42 a
    45042.27 b20.58 b3.82 c0.65 c2.31 b0.19 b
    同列数据后不同小写字母表示同一指标在不同浓度下差异显著(P<0.05),下同。
    Data with different lowercase letters on same column indicate significant differences on same indicator at different concentrations (P<0.05). Same for below.
    下载: 导出CSV

    表  3  Temuco成熟期不同浓度盐胁迫下农艺性状

    Table  3.   Agronomic characteristics of Temuco quinoa under salt stress at mature stage

    NaCl浓度
    NaCl concentration/(mmol·L−1)
    株高
    Plant height/cm
    叶面积
    leaf area/cm2
    叶片相对含水量
    Relative water content of leaves/%
    千粒重
    thousand kernel weight/g
    0157.2 a952.3 a91.5 a4.11 a
    200155.6 a947.8 a87.7 a4.02 a
    450131.9 b801.1 b80.2 b4.08 a
    下载: 导出CSV

    表  4  Temuco种子各元素成分含量

    Table  4.   Contents of elements in Temuco seeds

    NaCl浓度
    NaCl concentration/(mmol·L−1)
    N/%C/%S/%Fe/(mg·g−1)Cu/(mg·g−1)Ca/(mg·g−1)Mg/(mg·g−1)
    03.27 a44.82 a0.20 a0.058 a0.005 a8.592 a1.784 a
    2003.41 a45.03 a0.23 a0.060 a0.006 a8.439 a1.692 a
    4502.74 b44.91 a0.21 a0.057 a0.006 a8.682 a1.729 a
    下载: 导出CSV

    表  5  不同NaCl浓度处理后幼苗叶片生理生化指标

    Table  5.   Physiochemical indexes of seedling leaves treated with NaCl solutions of varied concentrations

    NaCl浓度
    NaCl concentration/
    ( mmol·L−1)
    SOD活性
    SOD Activity/
    (U·g−1·min−1)
    POD活性
    POD Activity/
    (U·g−1·min−1)
    可溶性糖含量
    Soluble suger content/
    (mg·g−1)
    脯氨酸含量
    Proline content/
    (μg·g−1)
    MDA含量
    MDA content/
    (μmol·g−1)
    0115.16 b75.32 b7.20 c25.12 b3.42 b
    200138.75 a96.37 a12.10 a84.75 a3.54 b
    45092.08 c53.28 c10.20 b72.84 a5.57 a
    下载: 导出CSV
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出版历程
  • 收稿日期:  2023-04-21
  • 修回日期:  2023-05-21
  • 网络出版日期:  2023-10-25
  • 刊出日期:  2023-10-28

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