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艾GRF基因家族的鉴定与表达分析

郭晨宁 李晶晶 杨晶凡 刘俊 杨灏 马蕊 陈随清 练从龙

郭晨宁,李晶晶,杨晶凡,等. 艾GRF基因家族的鉴定与表达分析 [J]. 福建农业学报,2024,39(5):1−10
引用本文: 郭晨宁,李晶晶,杨晶凡,等. 艾GRF基因家族的鉴定与表达分析 [J]. 福建农业学报,2024,39(5):1−10
GUO C N, LI J J, YANG J F, et al. Genome-wide Identification and Expression Analysis of AaGRF Gene Family in Artemisia argyi [J]. Fujian Journal of Agricultural Sciences,2024,39(5):1−10
Citation: GUO C N, LI J J, YANG J F, et al. Genome-wide Identification and Expression Analysis of AaGRF Gene Family in Artemisia argyi [J]. Fujian Journal of Agricultural Sciences,2024,39(5):1−10

艾GRF基因家族的鉴定与表达分析

基金项目: 河南省高等学校重点科研项目(22A360012);河南省中药材产业技术体系(豫科项[2022]30号);国家自然科学基金项目(82204551)。
详细信息
    作者简介:

    郭晨宁(1999 —),女,硕士研究生,主要从事药用植物分子生物学研究,E-mail:gcning6850@163.com

    通讯作者:

    练从龙(1988 —),男,博士,讲师,主要从事药用植物生理生态与分子生物学研究,E-mail:lian1988@hactcm.edu.cn

Genome-wide Identification and Expression Analysis of AaGRF Gene Family in Artemisia argyi

  • 摘要:   目的  生长调节因子(Growth-regulating factors, GRF)是植物的一种特有蛋白,深入了解艾GRF基因家族的生物学特性,对艾的生长发育和逆境胁迫有着重要作用。  方法  本研究对艾GRF基因家族进行理化性质、进化、基因结构以及表达等进行生物信息学进行分析,进而通过转录组数据和实时荧光定量PCR(qRT-PCR)技术分析基因家族各成员在不同组织、逆境胁迫以及响应不同激素的表达模式进行分析。  结果  从艾基因组中共鉴定出17个GRF家族成员,理化鉴别显示均为亲水蛋白;系统发育进化树分为四个亚家族;motif分析表明进化树中同一分支的基因具有相似或相同的保守基序;AaGRF基因家族成员随机分布在11条不同的染色体上;AaGRF基因家族启动子含有多种逆境和激素响应元件;AaGRF基因家族在响应盐碱胁迫时表现出不同的表达趋势;该家族成员在艾的根、茎、叶中的表达模式具有组织特异性;经脱落酸、吲哚乙酸、水杨酸和茉莉酸甲酯处理后,AaGRF05、AaGRF06、AaGRF11AaGRF14在处理后的12h基因表达量最高,这表明大部分基因在前期参与不同激素的调控。  结论  艾GRF基因家族参与艾生长发育、激素与逆境胁迫调节,为下一步全面探究GRF家族功能提供一定的理论依据。
  • 图  1  AaGRF家族系统进化树

    AT:拟南芥;Gh:棉花;Ha:向日葵;Aa:艾

    Figure  1.  Phylogenetic tree of AaGRF

    AT: Arabidopsis thaliana; Gh: Gossypium hirsutum L; Ha:Helianthus annuus L.; Aa: Artemisia argyi

    图  2  艾GRF基因家族的结构、结构域和motif

    Figure  2.  Structure, domains, and motif of the AaGRF gene family

    图  3  拟南芥GRF家族基因组结构、保守结构域和motif分析

    Figure  3.  Gene structure, conserved domains, and motif analysis of GRF gene family in Arabidopsis thaliana

    图  4  AaGRF基因家族在染色体上的定位

    Figure  4.  Mapping of the AaGRF gene family on the chromosome

    图  5  AaGRF基因家族顺式作用元件

    Figure  5.  The AaGRF family of gene in cis-acting elements

    图  6  AaGRF基因家族响应盐和盐碱胁迫的表达模式

    CK:空白对照组;SAT:盐碱处理组;ST:盐处理组;L:叶;R:根;

    Figure  6.  The expression pattern of AaGRF in response to salt and saline-alkali stress

    CK : blank control group ; SAT : saline-alkali treatment group ; ST : salt treatment group ; L : leaf ; R : root ;

    图  7  AaGRF家族成员在艾的根、茎、叶中的表达模式

    小写字母代表在 0.05 水平上差异显著。

    Figure  7.  Expression pattern of AaGRF family members in the roots, stems, and leaves of Artemisia argyi

    Data with lowercase letters represent significant differences at P<0.05.

    图  8  AaGRF家族成员响应激素处理的表达模式

    小写字母代表在 0.05 水平上差异显著。

    Figure  8.  Expression patterns of AaGRF family members in response to hormone treatment

    Data with lowercase letters represent significant differences at P<0.05.

    表  1  AaGRF基因家族蛋白理化性质

    Table  1.   Physiochemical properties of the AaGRF gene family proteins

    基因名称
    Gene name
    基因组ID
    genome ID
    长度
    length/aa
    分子量
    molecular weight /Da
    等电点
    Isoelectric point(pl)
    不稳定指数
    Instability index
    脂肪族指数
    Aliphatic index
    疏水指数
    Hydrophobic index
    AaGRF01 Aarg02G031430.1 350 39886.41 8.35 51.62 50.43 −0.921
    AaGRF02 Aarg04G019450.1 471 50852.5 9.07 41.98 64.86 −0.547
    AaGRF03 Aarg05G013120.1 315 36666.98 7.26 71.34 58.83 −0.9
    AaGRF04 Aarg06G029080.1 364 40414.66 8.87 48.27 52.77 −0.91
    AaGRF05 Aarg06G030040.1 394 43558.38 9 48.32 57.92 −0.717
    AaGRF06 Aarg06G030730.1 380 42917.36 8.13 56.92 54.92 −0.874
    AaGRF07 Aarg08G013810.1 138 15560.65 7.66 52.91 65.72 −0.541
    AaGRF08 Aarg08G014980.1 339 38274.79 8.02 49.95 43.42 −0.871
    AaGRF09 Aarg10G061780.1 335 38176.52 8.79 49.88 52.39 −0.87
    AaGRF10 Aarg11G000710.1 329 37157.12 8.97 47 52.52 −0.829
    AaGRF11 Aarg11G015240.1 501 54688.51 7.24 42.86 59.38 −0.635
    AaGRF12 Aarg12G018140.1 472 51001.69 9.16 42.64 64.51 −0.56
    AaGRF13 Aarg14G027820.1 364 40379.65 8.72 45.86 51.43 −0.898
    AaGRF14 Aarg14G028850.1 352 38751.75 8.44 51.96 54.29 −0.709
    AaGRF15 Aarg16G013730.1 138 15512.59 7.66 52.9 69.28 −0.509
    AaGRF16 Aarg16G015010.1 339 38280.78 7.66 49.46 45.13 −0.853
    AaGRF17 Aarg17G035750.1 382 41725.01 6.67 45.33 56.18 −0.678
    下载: 导出CSV
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  • 收稿日期:  2024-03-19
  • 修回日期:  2024-04-16
  • 网络出版日期:  2024-06-26

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