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含ACC脱氨酶的根际促生菌对切花月季生长的影响

李文祥 张天谣 李洋 李新艺 黄海泉 黄美娟

李文祥,张天谣,李洋,等. 含ACC脱氨酶的根际促生菌对切花月季生长的影响 [J]. 福建农业学报,2023,38(4):423−430 doi: 10.19303/j.issn.1008-0384.2023.04.005
引用本文: 李文祥,张天谣,李洋,等. 含ACC脱氨酶的根际促生菌对切花月季生长的影响 [J]. 福建农业学报,2023,38(4):423−430 doi: 10.19303/j.issn.1008-0384.2023.04.005
LI W X, ZHANG T Y, LI Y, et al. Effect of Rhizobacteria Containing ACC Deaminase on Growth of Rose Bush [J]. Fujian Journal of Agricultural Sciences,2023,38(4):423−430 doi: 10.19303/j.issn.1008-0384.2023.04.005
Citation: LI W X, ZHANG T Y, LI Y, et al. Effect of Rhizobacteria Containing ACC Deaminase on Growth of Rose Bush [J]. Fujian Journal of Agricultural Sciences,2023,38(4):423−430 doi: 10.19303/j.issn.1008-0384.2023.04.005

含ACC脱氨酶的根际促生菌对切花月季生长的影响

doi: 10.19303/j.issn.1008-0384.2023.04.005
基金项目: 国家林业和草原局推广项目(2020133126);国家自然科学基金(31760001);云南省自然科学基金(2011FB067);云南省园林植物遗传改良与高效繁育博士生导师团队项目(503210103)
详细信息
    作者简介:

    李文祥(1995−),男,硕士研究生,研究方向:园林植物研究(E-mail:972403487@qq.com

    通讯作者:

    黄海泉(1974−),男,教授,研究方向:园林植物研究(E-mail:haiquanl@163.com

    黄美娟(1972−),女,教授,研究方向:园林植物研究(E-mail:xmhhq2001@163.com

  • 中图分类号: S685.12

Effect of Rhizobacteria Containing ACC Deaminase on Growth of Rose Bush

  • 摘要:   目的  探明含ACC脱氨酶(1-aminocyclopropane-1-carboxylate deaminase)的不同植物根际促生菌对切花月季白荔枝的促生效果。  方法  以白荔枝为试验材料,在其盆栽过程中施入含ACC脱氨酶的不同PGPR菌株,通过测定其株高、茎粗、分枝数等农艺性状、叶绿素a/b值、叶片保护酶活性及MDA含量、花朵乙烯释放量、光合及叶绿素荧光参数等生理指标,探讨不同菌株对白荔枝生长及生理的影响。  结果  施入含ACC脱氨酶的不同PGPR菌株对白荔枝生长及光合作用产生的影响有所差异,其中菌株F23和F195处理较对照株高增加25.9%和26.0%,且F23处理后茎粗比对照处理增加17.1%,菌株F23和F195处理叶绿素a/b也高于对照。菌株F23显著提高了根际土壤脲酶、磷酸酶和蔗糖酶活性至42.6%、 16.3%和48.8%;且菌株F23对POD以及CAT活性的提高有显著促进作用,分别达到对照的1.78和2.07倍,花朵乙烯释放量比对照减少37.8%。净光合速率经菌株F195和F23处理后显著高于对照,分别达对照的1.40倍和1.16倍。  结论  综合比较认为,菌株F23对切花月季白荔枝的促生效果最好。
  • 图  1  含ACC脱氨酶的不同PGPR菌株对白荔枝叶片叶绿素a/b(A)及MDA含量(B)的影响

    Figure  1.  Effects of PGPR on leaf chlorophyll a/b (A) and MDA (B) of White Lichee

    图  2  含ACC脱氨酶的不同PGPR菌株对白荔枝花朵乙烯释放量及叶片抗氧化酶活性的影响

    Figure  2.  Effects of PGPR on floral ethylene production and leaf antioxidant enzyme activities of White Lichee

    表  1  含ACC脱氨酶的不同菌株信息

    Table  1.   Information on PGPR containing ACC deaminase

    菌株编号
    Strain number
    属名
    Genus
    ACC脱氨酶活性
    ACC deaminase activity/(U·mg−1)
    F195Pseudomonas sp.6.6910
    F105Stenotrophomonas sp.0.9600
    F11Bacillus sp.1.1342
    F23Arthrobacter sp.1.0359
    L11Stenotrophomonas sp.1.0356
    L74Stenotrophomonas sp.1.0363
    Z22Bacillus sp.1.0361
    下载: 导出CSV

    表  2  含ACC脱氨酶的不同PGPR菌株对白荔枝主要农艺性状的影响

    Table  2.   Effects of PGPR on main agronomic characteristics of White Lichee

    处理
    Treatment
    株高
    Plant height/cm
    茎粗
    Stem diameter/mm
    分枝数
    Branch number
    花径
    Flower diameter/mm
    花期
    Flower period/d
    F19584.52±6.32 a7.21±1.25 ab1.33±0.14 a129.68±11.67 a15.33±2.08 a
    F10581.52±8.48 a6.99±1.02 ab1.67±0.63 a119.06±10.89 a13.00±1.00 ab
    F1187.66±2.46 a7.59±1.35 ab1.67±0.63 a114.49±5.25 a16.00±1.00 a
    F2384.47±6.89 a8.02±1.53 a1.42±0.38 a118.94±2.90 a15.33±2.08 a
    L1189.56±6.49 a7.45±0.60 ab1.42±0.14 a116.95±15.21 a13.33±2.31 ab
    L7484.47±10.25 a7.16±1.23 ab1.75±0.25 a114.71±9.70 a13.67±1.53 ab
    Z2289.12±5.77 a6.97±0.99 ab1.75±0.00 a122.06±5.21 a11.67±1.16 b
    Mix80.85±5.44 a7.20±1.39 ab1.58±0.29 a121.12±4.27 a13.33±1.53 ab
    CK67.10±1.52 b6.85±1.44 b2.00±0.25 a117.42±0.87 a14.33±0.58 ab
    表内数据表示平均值±标准差,同列不同小写字母表示差异显著(P<0.05),3个生物学重复。下同。
    Data are mean ± SD; those with different lowercase letters on same column indicate significant differences on biological triplicates (P<0.05). Three biological replicates. Same for below.
    下载: 导出CSV

    表  3  含ACC脱氨酶的不同PGPR菌株对白荔枝根际土壤酶活的影响

    Table  3.   Effect of PGPR on soil enzyme activity in rhizosphere of White Lichee

    处理
    Treatment
    脲酶活性
    Urease activity/
    (mg·g−1·d−1)
    磷酸酶活性
    Phosphatase activity/
    (mg·g−1·d−1)
    蔗糖酶活性
    Sucrase activity/
    (mg·g−1·d−1)
    F1950.355±0.011 a1.035±0.011 a22.474±0.820 b
    F1050.278±0.030 c0.764±0.007 e12.392±1.325 c
    F110.229±0.016 d0.993±0.008 b12.458±0.594 c
    F230.338±0.015 ab1.041±0.007 a33.475±2.000 a
    L110.295±0.032 c0.886±0.010 c11.374±0.802 cd
    L740.308±0.011 bc0.991±0.011 b9.863±0.205 d
    Z220.237±0.012 d0.884±0.001 c6.809±1.312 e
    Mix0.277±0.023 c0.790±0.005 d7.006±1.035 e
    CK0.237±0.012 d0.895±0.010 c7.433±1.133 e
    下载: 导出CSV

    表  4  含ACC脱氨酶的不同PGPR菌株对白荔枝光合特性的影响

    Table  4.   Effect of PGPR on photosynthesis of White Lichee

    处理
    Treatment
    净光合速率
    Pn/(μmol·m−2·s−1)
    气孔导度
    Gs/(mol·m−2·s−1)
    胞间CO2浓度
    Ci/(μmol·mol−1)
    蒸腾速率
    Tr/(mmol·m−2·s−1)
    水分利用效率
    Pn/Tr
    F1959.34±0.45 a0.278±0.094 a427±33.78 a3.19±1.19 a3.18±1.07 a
    F1056.32±0.14 d0.084±0.034 c362±21.17 bc1.90±0.83 a3.68±1.23 a
    F117.11±0.09 c0.077±0.023 c363±6.43 bc1.77±0.61 a4.39±1.62 a
    F237.79±0.35 b0.120±0.020 bc387±18.25 abc2.18±0.82 a3.94±1.57 a
    L116.97±0.28 c0.093±0.032 c376±54.60 abc2.38±1.81 a4.01±2.17 a
    L747.98±0.16 b0.181±0.063 b404±17.62 abc3.64±1.27 a2.41±0.96 a
    Z226.84±0.22 c0.089±0.016 c353±15.04 c1.83±0.56 a3.96±1.06 a
    Mix7.81±0.34 b0.135±0.036 bc411±25.54 bc2.24±0.96 a3.84±1.27 a
    CK6.69±0.09 cd0.106±0.035 bc389±25.06 abc1.81±0.58 a4.02±1.52 a
    下载: 导出CSV

    表  5  含ACC脱氨酶的不同PGPR菌株对白荔枝叶绿素荧光特性的影响

    Table  5.   Effect of PGPR on chlorophyll fluorescence of White Lichee

    处理
    Treatment
    最大光化学效率
    Fv/Fm
    实际光化学效率
    ΦPSII
    光化学淬灭系数
    qP
    非光化学淬灭系数
    qN
    表观光合电子传递效率ETR
    F1950.787±0.019 a0.566±0.029 d0.293±0.067 a0.805±0.054 a3.073±0.416 c
    F1050.789±0.028 a0.692±0.021 ab0.324±0.056 a0.726±0.081 a7.953±2.790 ab
    F110.777±0.017 a0.618±0.064 cd0.251±0.052 a0.782±0.031 a4.880±1.750 bc
    F230.800±0.021 a0.709±0.027 ab0.311±0.024 a0.812±0.040 a6.147±1.684 abc
    L110.776±0.015 a0.667±0.037 bc0.339±0.022 a0.783±0.030 a5.640±0.737 bc
    L740.780±0.019 a0.659±0.019 bc0.348±0.061 a0.797±0.090 a5.567±1.362 bc
    Z220.780±0.020 a0.642±0.067 bc0.304±0.076 a0.781±0.057 a3.867±0.167 c
    Mix0.808±0.013 a0.740±0.025 a0.343±0.046 a0.788±0.067 a9.353±2.136 a
    CK0.794±0.001 a0.420±0.024 e0.330±0.045 a0.721±0.064 a7.900±3.251 ab
    下载: 导出CSV
  • [1] 徐雪东, 张超, 秦成, 等. 干旱下接种根际促生细菌对苹果实生苗光合和生理生态特性的影响 [J]. 应用生态学报, 2019, 30(10):3501−3508.

    XU X D, ZHANG C, QIN C, et al. Effects of PGPR inoculation on photosynthesis and physiological-ecological characteristics of apple seedlings under drought stress [J]. Chinese Journal of Applied Ecology, 2019, 30(10): 3501−3508.(in Chinese)
    [2] 戚秀秀, 魏畅, 刘晓丹, 等. 根际促生菌应用于基质对水稻幼苗生长的影响 [J]. 土壤, 2020, 52(5):1025−1032.

    QI X X, WEI C, LIU X D, et al. Effects of plant growth-promoting rhizobacteria added in seedling substrate on rice growth [J]. Soils, 2020, 52(5): 1025−1032.(in Chinese)
    [3] GLICK B R. Bacteria with ACC deaminase can promote plant growth and help to feed the world [J]. Microbiological Research, 2014, 169(1): 30−39. doi: 10.1016/j.micres.2013.09.009
    [4] 赵龙飞, 徐亚军, 常佳丽, 等. 具ACC脱氨酶活性大豆根瘤内生菌的筛选、抗性及促生作用 [J]. 微生物学报, 2016, 56(6):1009−1021.

    ZHAO L F, XU Y J, CHANG J L, et al. Screening, resistance and growth-promoting effect of endophytic bacteria with ACC deaminase activity isolated from soybean nodules [J]. Acta Microbiologica Sinica, 2016, 56(6): 1009−1021.(in Chinese)
    [5] 王伟楠, 兰智勇, 喻文丽, 等. 盐穗木根际产ACC脱氨酶耐盐菌株的筛选及鉴定 [J]. 中国土壤与肥料, 2021(2):270−275. doi: 10.11838/sfsc.1673-6257.20057

    WANG W N, LAN Z Y, YU W L, et al. Screening and identification of salt-tolerant and ACC deaminase-producing strains in Halostachys caspica rhizosphere [J]. Soil and Fertilizer Sciences in China, 2021(2): 270−275.(in Chinese) doi: 10.11838/sfsc.1673-6257.20057
    [6] KUMAR A, MALEVA M, BRUNO L B, et al. Synergistic effect of ACC deaminase producing Pseudomonas sp. TR15a and siderophore producing Bacillus aerophilus TR15c for enhanced growth and copper accumulation in Helianthus annuus L [J]. Chemosphere, 2021, 276: 130038. doi: 10.1016/j.chemosphere.2021.130038
    [7] SHAHZAD S M, ARIF M S, RIAZ M, et al. PGPR with varied ACC-deaminase activity induced different growth and yield response in maize (Zea mays L. ) under fertilized conditions [J]. European Journal of Soil Biology, 2013, 57: 27−34. doi: 10.1016/j.ejsobi.2013.04.002
    [8] 费诗萱, 张敏, 王迎, 等. 具有ACC脱氨酶活性的红枣根际促生菌株的分离筛选及其促生效果研究 [J]. 西北林学院学报, 2019, 34(6):140−146. doi: 10.3969/j.issn.1001-7461.2019.06.22

    FEI S X, ZHANG M, WANG Y, et al. Isolation, screening and promoting effects of plant growth-promoting rhizobacteria (PGPR) containing ACC deaminase from jujube [J]. Journal of Northwest Forestry University, 2019, 34(6): 140−146.(in Chinese) doi: 10.3969/j.issn.1001-7461.2019.06.22
    [9] 谭程仁. 切花月季环保生产现状与发展对策研究[D]. 昆明: 云南大学, 2017.

    TAN C R. Research on the current situation and countermeasures of environmentally friendly production of rose hybrida[D]. Kunming: Yunnan University, 2017. (in Chinese)
    [10] 赵凤亮, 邹刚华, 单颖, 等. 香蕉园化肥施用现状、面源污染风险及其养分综合管理措施 [J]. 热带作物学报, 2020, 41(11):2346−2352. doi: 10.3969/j.issn.1000-2561.2020.11.028

    ZHAO F L, ZOU G H, SHAN Y, et al. Current status of chemical fertilizer application in banana plantation, environmental risks and integrated nutrient management practices [J]. Chinese Journal of Tropical Crops, 2020, 41(11): 2346−2352.(in Chinese) doi: 10.3969/j.issn.1000-2561.2020.11.028
    [11] 高俊凤. 植物生理学实验指导[M]. 北京: 高等教育出版社, 2006.
    [12] 关松荫. 土壤酶及其研究法[M]. 北京: 农业出版社, 1986.
    [13] 舒健虹, 刘晓霞, 王子苑, 等. 不同氮磷条件下施加促生菌对多花黑麦草生长的影响 [J]. 中国草地学报, 2021, 43(2):28−36.

    SHU J H, LIU X X, WANG Z Y, et al. Effects of growth-promoting bacteria on the growth of Lolium multiflorum under different nitrogen and phosphorus conditions [J]. Chinese Journal of Grassland, 2021, 43(2): 28−36.(in Chinese)
    [14] OLANREWAJU O S, GLICK B R, BABALOLA O O. Mechanisms of action of plant growth promoting bacteria [J]. World Journal of Microbiology and Biotechnology, 2017, 33(11): 197. doi: 10.1007/s11274-017-2364-9
    [15] 孙韵雅, 陈佳, 王悦, 等. 根际促生菌促生机理及其增强植物抗逆性研究进展 [J]. 草地学报, 2020, 28(5):1203−1215.

    SUN Y Y, CHEN J, WANG Y, et al. Advances in growth promotion mechanisms of PGPRs and their effects on improving plant stress tolerance [J]. Acta Agrestia Sinica, 2020, 28(5): 1203−1215.(in Chinese)
    [16] 贺字典, 闫立英, 石延霞, 等. 产生ACC脱氨酶的PGPR种衣剂对黄瓜细菌性茎软腐病的防治效果 [J]. 中国生物防治学报, 2017, 33(6):817−825.

    HE Z D, YAN L Y, SHI Y X, et al. Bio-control of PGPR seed coating producing ACC deaminase to cucumber bacterial stem soft rot disease [J]. Chinese Journal of Biological Control, 2017, 33(6): 817−825.(in Chinese)
    [17] 李美芳, 张平, 李倩, 等. 镉胁迫下两株产铁载体/解磷菌株对黑麦草种子萌发及幼苗积累镉的影响 [J]. 中南林业科技大学学报, 2021, 41(9):179−187.

    LI M F, ZHANG P, LI Q, et al. Effects of two siderophore producing/phosphorus solubilizing strains on seed germination and cadmium accumulation of Lolium perenne under cadmium stress [J]. Journal of Central South University of Forestry & Technology, 2021, 41(9): 179−187.(in Chinese)
    [18] OTEINO N, LALLY R D, KIWANUKA S, et al. Plant growth promotion induced by phosphate solubilizing endophytic Pseudomonas isolates [J]. Frontiers in Microbiology, 2015, 6: 745.
    [19] 孙海, 张淋淋, 金桥, 等. 基于非靶向代谢组学分析假单胞菌P1解磷作用 [J]. 西北农业学报, 2019, 28(9):1452−1459.

    SUN H, ZHANG L L, JIN Q, et al. Elucidating phosphorus dissolving action of Pseudomonas P1 by non-targeted metabolomics [J]. Acta Agriculturae Boreali-Occidentalis Sinica, 2019, 28(9): 1452−1459.(in Chinese)
    [20] DEL CARMEN OROZCO-MOSQUEDA M, GLICK B R, SANTOYO G. ACC deaminase in plant growth-promoting bacteria (PGPB): An efficient mechanism to counter salt stress in crops [J]. Microbiological Research, 2020, 235: 126439. doi: 10.1016/j.micres.2020.126439
    [21] JIN K M, LI H B, LI X Q, et al. Rhizosphere bacteria containing ACC deaminase decrease root ethylene emission and improve maize root growth with localized nutrient supply [J]. Food and Energy Security, 2021, 10(2): 275−284. doi: 10.1002/fes3.278
    [22] GUPTA A, RAI S, BANO A, et al. ACC deaminase produced by PGPR mitigates the adverse effect of osmotic and salinity stresses in Pisum sativum through modulating the antioxidants activities [J]. Plants, 2022, 11(24): 3419. doi: 10.3390/plants11243419
    [23] 谢显秋, 张瑞楠, 韦江璐, 黄毓燕, 陈炯宇, 李杨瑞, 邢永秀. 4株甘蔗固氮菌株活化土壤养分及影响土壤酶活的分析[J/OL]. 分子植物育种: 1-27[2023-05-19]. http://kns.cnki.net/kcms/detail/46.1068.S.20210607.1338.008.html.

    XIE X Q, ZHANG R N, WEI J L, HUANG Y Y, CHEN J Y, LI Y R, XING Y X. Analysis of four nitrogen fixing sugarcane strains activating soil nutrients and affecting soil enzymes[J/OL]. Molecular Plant Breeding: 1-27[2023-05-19]. http://kns.cnki.net/kcms/detail/46.1068.S.20210607.1338.008.html.
    [24] 王梦园, 杜延全, 朱建强. 复合促生菌对小麦苗期生长和土壤酶活的影响 [J]. 中国农业科技导报, 2019, 21(10):98−106.

    WANG M Y, DU Y Q, ZHU J Q. Influences compound probiotics on wheat growth in the seedling stage and soil enzyme activity [J]. Journal of Agricultural Science and Technology, 2019, 21(10): 98−106.(in Chinese)
    [25] 赵雨萌, 缪佩佩, 王旭东, 等. 解淀粉芽胞杆菌TR2对草莓土壤酶活性的影响与防病促生作用 [J]. 中国生物防治学报, 2022, 38(2):495−501.

    ZHAO Y M, MIAO P P, WANG X D, et al. Influences of Bacillus amyloliquefaciens TR2 on soil enzyme activities and its effects on disease control and growth promotion in strawberry [J]. Chinese Journal of Biological Control, 2022, 38(2): 495−501.(in Chinese)
    [26] 曾文芳, 李亚姝, 崔晓宁, 等. 施氮对紫花苜蓿光合作用及抗蓟马的影响 [J]. 草原与草坪, 2021, 41(1):61−66,75. doi: 10.13817/j.cnki.cyycp.2021.01.009

    ZENG W F, LI Y S, CUI X N, et al. Effect of nitrogen application on photosynthetic characteristics and resistance of alfalfa to thrips [J]. Grassland and Turf, 2021, 41(1): 61−66,75.(in Chinese) doi: 10.13817/j.cnki.cyycp.2021.01.009
    [27] 袁宗胜, 刘芳, 黄秋良, 等. 内生细菌对芳樟光合特性和几种酶活性的作用 [J]. 基因组学与应用生物学, 2019, 38(8):3559−3565.

    YUAN Z S, LIU F, HUANG Q L, et al. The effect of entophytic bacteria on the photosynthetic characteristics and several enzyme activities of Cinnamomum camphora [J]. Genomics and Applied Biology, 2019, 38(8): 3559−3565.(in Chinese)
    [28] WONG S C, COWAN I R, FARQUHAR G D. Stomatal conductance correlates with photosynthetic capacity [J]. Nature, 1979, 282(5737): 424−426. doi: 10.1038/282424a0
    [29] 钱申, 王志侠, 陈慧妹, 等. 不同微生境中水光温变化对毛尖紫萼藓叶绿素荧光特性的影响 [J]. 生态学报, 2021, 41(4):1482−1491.

    QIAN S, WANG Z X, CHEN H M, et al. Effects of water-light-temperature changes in different microhabitats on chlorophyll fluorescence characteristics of Grimmia pilifera [J]. Acta Ecologica Sinica, 2021, 41(4): 1482−1491.(in Chinese)
    [30] 吕德国, 于翠, 秦嗣军, 等. 本溪山樱根部解磷细菌的定殖规律及其对植株生长发育的影响 [J]. 中国农业科学, 2008, 41(2):508−515. doi: 10.3864/j.issn.0578-1752.2008.02.026

    LÜ D G, YU C, QIN S J, et al. Colonization regulation pattern of phosphobacteria and its effect on the growth and development of Cerasus sachalinensis [J]. Scientia Agricultura Sinica, 2008, 41(2): 508−515.(in Chinese) doi: 10.3864/j.issn.0578-1752.2008.02.026
    [31] 秦嗣军, 张硕, 周文杰, 等. 根际促生细菌对东北山樱幼苗光合特性及生长的影响 [J]. 果树学报, 2014, 31(S1):98−102.

    QIN S J, ZHANG S, ZHOU W J, et al. Effect of plant growth promoting rhizobacteria on photosynthesis and growth of Cerasus sachalinensis seedlings [J]. Journal of Fruit Science, 2014, 31(S1): 98−102.(in Chinese)
    [32] PARÁDI I, BRATEK Z, LÁNG F. Influence of arbuscular mycorrhiza and phosphorus supply on polyamine content, growth and photosynthesis of Plantago lanceolata [J]. Biologia Plantarum, 2003, 46(4): 563−569. doi: 10.1023/A:1024819729317
    [33] 汪敦飞, 郑新宇, 肖清铁, 等. 铜绿假单胞菌对镉胁迫苗期水稻根系活力及叶片生理特性的影响 [J]. 应用生态学报, 2019, 30(8):2767−2774. doi: 10.13287/j.1001-9332.201908.037

    WANG D F, ZHENG X Y, XIAO Q T, et al. Effects of Pseudomonas aeruginosa on root activity and leaf physiological characteristics in rice (Oryza sativa L. ) seedling under cadmium stress [J]. Chinese Journal of Applied Ecology, 2019, 30(8): 2767−2774.(in Chinese) doi: 10.13287/j.1001-9332.201908.037
    [34] FERREIRA C M H, SOARES H M V M, SOARES E V. Promising bacterial Genera for agricultural practices: An insight on plant growth-promoting properties and microbial safety aspects [J]. Science of the Total Environment, 2019, 682: 779−799. doi: 10.1016/j.scitotenv.2019.04.225
    [35] 林斌, 黄菊青, 官雪芳, 等. 解淀粉芽孢杆菌液体肥在茶叶上的应用研究 [J]. 福建农业学报, 2019, 34(10):1173−1178. doi: 10.19303/j.issn.1008-0384.2019.10.009

    LIN B, HUANG J Q, GUAN X F, et al. Application of Bacillus amyloliquefaciens liquid fertilizer on tea bushes [J]. Fujian Journal of Agricultural Sciences, 2019, 34(10): 1173−1178.(in Chinese) doi: 10.19303/j.issn.1008-0384.2019.10.009
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出版历程
  • 收稿日期:  2022-01-12
  • 修回日期:  2023-03-29
  • 网络出版日期:  2023-05-09
  • 刊出日期:  2023-04-28

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