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外源槲皮素对干旱胁迫下板栗苗抗氧化的影响

曾扬鹃 于克妍 靳常敏 于立洋 张京政 曹飞

曾扬鹃,于克妍,靳常敏,等. 外源槲皮素对干旱胁迫下板栗苗抗氧化的影响 [J]. 福建农业学报,2024,39(X):1−7
引用本文: 曾扬鹃,于克妍,靳常敏,等. 外源槲皮素对干旱胁迫下板栗苗抗氧化的影响 [J]. 福建农业学报,2024,39(X):1−7
ZENG Y J, YU K Y, JIN C M, et al. Effect of exogenous quercetin on antioxidant of chestnut seedlings under drought stress [J]. Fujian Journal of Agricultural Sciences,2024,39(X):1−7
Citation: ZENG Y J, YU K Y, JIN C M, et al. Effect of exogenous quercetin on antioxidant of chestnut seedlings under drought stress [J]. Fujian Journal of Agricultural Sciences,2024,39(X):1−7

外源槲皮素对干旱胁迫下板栗苗抗氧化的影响

基金项目: 国家重点研发计划项目(2022YFD2200400);教育部工程研究中心项目(PT2022-01)。
详细信息
    作者简介:

    曾扬鹃(2000 —),女,硕士研究生,主要从事板栗栽培与育种研究,E-mail:zengyangjuan0613@163.com

    通讯作者:

    曹飞(1988 —),男,博士,讲师,主要从事果树育种和栽培生理教学与科研等工作,E-mail:caofeiqhd@126.com

  • 中图分类号: S664.2

Effect of exogenous quercetin on antioxidant of chestnut seedlings under drought stress

  • 摘要:   目的  探寻外源槲皮素(Quercetin, Q)对提高板栗苗耐旱性的生理机制和适宜喷施浓度。  方法  以燕宝(YB)板栗苗为试材,通过盆栽控水的方法,以重度干旱胁迫CK1和正常管理CK2为对照,研究在重度干旱胁迫下喷施不同浓度[600 μmol·L−1(QD1)、1200 μmol·L−1(QD2)、1800 μmol·L−1(QD3)和2000 μmol·L−1(QD4)、3000 μmol·L−1(QD5)]槲皮素对板栗幼苗叶绿素含量、抗氧化酶活性、丙二醛(MDA)和黄酮醇含量的影响。  结果  与同处于重度干旱胁迫下未经槲皮素喷施处理的CK1相比,5种不同浓度槲皮素喷施处理均能显著提高板栗叶片叶绿素含量、超氧化物歧化酶(SOD)活性、过氧化氢酶(CAT)活性、过氧化物酶(POD)活性和黄酮醇含量,同时显著降低了板栗叶片丙二醛(MDA)含量,但喷施后的板栗苗的状态仍不能恢复到正常管理的CK2。通过主成分分析及隶属函数法,得出QD1、QD2、QD3、QD4、QD5各处理的平均抗旱性度量值分别为0.735、0.641、0.738、1.389、0.828。其中,2000 μmol·L−1(QD4)槲皮素浓度处理效果更显著。  结论  外源施加槲皮素可以通过提高板栗幼苗抗氧化酶活性、叶绿素含量,减缓MDA增加速度,减缓细胞膜质过氧化的速度来减轻干旱对板栗叶片细胞的伤害,从而提高板栗幼苗耐旱性,且以2000 μmol·L−1(QD4)槲皮素浓度处理效果最佳。
  • 表  1  干旱胁迫下喷施槲皮素后板栗叶片叶绿素含量的变化

    Table  1.   Changes of chlorophyll content in chestnut leaves after spraying quercetin under drought stress                 (单位:mg·g−1)

    处理
    Handle
    时间
    Time
    0 d 5 d 10 d
    CK1 3.53±0.01cd 3.19±0.03e 1.50±0.03f
    CK2 5.12±0.07a 5.13±0.04a 5.30±0.06a
    QD1 3.49±0.02 de 4.71±0.04 d 4.15±0.10 d
    QD2 3.66±0.01b 4.91±0.05c 4.15±0.01 d
    QD3 3.63±0.03b 5.03±0.02b 4.26±0.01c
    QD4 3.44±0.01e 5.05±0.01b 4.75±0.01b
    QD5 3.57±0.01c 4.64±0.01 d 4.05±0.01e
    不同小写字母表示同列数值差异显著(P<0.01),下同
    Different lowercase letters indicate significant differences in numerical values within the same column(P<0.01). The same below.
    下载: 导出CSV

    表  2  干旱胁迫下喷施槲皮素后板栗叶片MDA含量的变化

    Table  2.   Changes of MDA content in chestnut leaves after spraying quercetin under drought stress                (单位:nmol·g−1)

    处理
    Handle
    时间
    Time
    0 d 5 d 10 d
    CK1 63.12±1.23 d 131.28±2.95a 157.29±7.23a
    CK2 37.50±2.13e 39.90±1.04 d 41.28±0.42e
    QD1 68.71±6.09cd 91.07±1.72b 107.50±0.44b
    QD2 73.70±0.32abc 87.20±2.23bc 102.17±1.26bc
    QD3 76.63±3.74a 86.22±1.26c 97.65±1.79cd
    QD4 74.95±0.53ab 84.45±2.12c 93.22±1.49 d
    QD5 69.75±0.32bc 88.41±0.74bc 101.31±1.37bc
    下载: 导出CSV

    表  3  干旱胁迫下喷施槲皮素后板栗叶片SOD活性的变化

    Table  3.   Changes of SOD activity in chestnut leaves after spraying quercetin under drought stress                  (单位:U·g−1)

    处理
    Handle
    时间
    Time
    0 d 5 d 10 d
    CK1 1157.97±36.93b 1470.95±111.96 d 990.53±13.67e
    CK2 2034.94±61.22a 2107.45±37.58a 2054.98±42.42a
    QD1 1152.45±55.34b 1689.88±35.16c 1322.79±33.30 d
    QD2 1167.31±42.48b 1743.98±24.17c 1447.38±49.93c
    QD3 1080.96±55.52b 1739.51±16.11c 1431.06±21.96c
    QD4 1108.46±40.92b 1907.68±31.35b 1522.91±33.64b
    QD5 1133.02±21.24b 1753.36±44.91c 1320.27±35.56 d
    下载: 导出CSV

    表  4  干旱胁迫下喷施槲皮素后板栗叶片POD活性的变化

    Table  4.   Changes of POD activity in chestnut leaves after spraying quercetin under drought stress                  (单位:U·g−1)

    处理
    Handle
    时间
    Time
    0 d 5 d 10 d
    CK1 860.00±81.65b 893.33±57.35e 600.00±43.20f
    CK2 1660.00±101.98a 1646.67±24.94a 1746.67±92.86a
    QD1 846.67±67.99b 1066.67±9.43 de 760.00±28.28ef
    QD2 886.67±33.99b 1113.33±89.94 d 913.33±131.99 de
    QD3 893.33±77.17b 1346.67±108.73bc 1153.33±33.99bc
    QD4 900.00±28.28b 1506.67±133.00ab 1300.0071.18b
    QD5 786.67±33.99b 1206.67±73.64cd 1040.00±81.65cd
    下载: 导出CSV

    表  5  干旱胁迫下喷施槲皮素后板栗叶片CAT活性的变化

    Table  5.   Changes of CAT activity in chestnut leaves after spraying quercetin under drought stress               (单位:nmol·min−1·g−1)

    处理
    Handle
    时间
    Time
    0 d 5 d 10 d
    CK1 164.98±3.20b 183.06±5.54 d 133.34±20.96 d
    CK2 316.40±17.80a 318.66±29.29a 305.10±5.54a
    QD1 162.72±5.54b 244.08±14.65bc 207.92±22.37bc
    QD2 164.98±20.96b 223.74±5.54c 176.28±19.96c
    QD3 155.94±5.54b 219.22±3.20c 178.54±8.46c
    QD4 158.20±6.39b 262.16±25.57b 212.44±13.93b
    QD5 166.34±2.79b 228.26±8.46bc 196.62±9.59bc
    下载: 导出CSV

    表  6  干旱胁迫下喷施槲皮素后板栗叶片黄酮醇含量的变化

    Table  6.   Changes of Flavonol content in chestnut leaves after spraying quercetin under drought stress                   (单位:mg·g−1)

    处理
    Handle
    时间
    Time
    0 d 5 d 10 d
    CK1 2.00±0.03a 2.74±0.25 d 2.22±0.05b
    CK2 1.07±0.15b 1.09±0.12e 1.10±0.02c
    QD1 1.95±0.20a 2.97±0.18cd 2.55±0.21ab
    QD2 1.96±0.08a 3.24±0.21bc 2.56±0.03ab
    QD3 1.99±0.08a 3.15±0.19bcd 2.36±0.17ab
    QD4 1.86±0.17a 3.48±0.29ab 2.71±0.07a
    QD5 1.79±0.12a 3.86±0.04a 2.60±0.36a
    下载: 导出CSV

    表  7  干旱胁迫下外源喷施槲皮素5 d、10 d的主成分系数、特征值及贡献率

    Table  7.   The principal component coefficient, characteristic value and contribution rate of exogenous spraying quercetin for 5 days and 10 days under drought stress

    时间
    Time
    主成分
    Component
    X1 X2 X3 X4 X5 X6 特征值
    Eigenvalues
    贡献率
    Contribution rate /%
    累积贡献率
    Cumulative contribution rate /%
    5 d 1 0.955 0.954 −0.930 0.761 0.519 0.305 3.629 60.478 60.478
    2 0.002 0.230 0.145 −0.639 0.269 0.855 1.287 21.446 81.923
    10 d 1 0.498 −0.486 0.467 0.449 0.177 0.254 3.642 60.705 60.705
    2 0.112 0.240 −0.220 −0.241 0.687 0.592 1.655 27.582 88.287
    X1-叶绿素; X2-MDA; X3-SOD; X4-POD; X5-CAT; X6-黄酮醇。下表同。
    X1- chlorophyll; X2-MDA; X3-SOD; X4-POD; X5-CAT; X6-flavonol. The same as below.
    下载: 导出CSV

    表  8  各处理的抗旱性度量值及综合排序

    Table  8.   8 The Drought resistance membership function value and comprehensive ranking of each treatment

    处理
    Treatment
    5 d抗旱性
    度量值
    5 days Drought-
    tolerance value
    10 d抗旱性
    度量值
    10 days Drought-
    tolerance value
    平均抗旱性
    度量值
    Drought-
    tolerance
    value
    排序
    Order
    QD1 0.927 0.544 0.735 4
    QD2 0.711 0.570 0.641 5
    QD3 0.855 0.621 0.738 3
    QD4 0.961 1.817 1.389 1
    QD5 0.983 0.672 0.828 2
    下载: 导出CSV
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  • 收稿日期:  2023-12-21
  • 修回日期:  2024-03-04
  • 网络出版日期:  2024-05-08

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