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秸秆还田对稻田土壤磷素形态及磷循环微生物功能基因的影响

李季 张桥 张嘉欣 韩兴华 黎婉玲 孙丽丽 顾文杰 卢钰升 解开治 徐培智

李季,张桥,张嘉欣,等. 秸秆还田对稻田土壤磷素形态及磷循环微生物功能基因的影响 [J]. 福建农业学报,2023,38(10):1230−1241 doi: 10.19303/j.issn.1008-0384.2023.10.012
引用本文: 李季,张桥,张嘉欣,等. 秸秆还田对稻田土壤磷素形态及磷循环微生物功能基因的影响 [J]. 福建农业学报,2023,38(10):1230−1241 doi: 10.19303/j.issn.1008-0384.2023.10.012
LI J, ZHANG Q, ZHANG J X, et al. Effects of Straw-returning on Phosphorus Morphology and Microbial Phosphorus-cycling Genes in Rice Paddy Soil [J]. Fujian Journal of Agricultural Sciences,2023,38(10):1230−1241 doi: 10.19303/j.issn.1008-0384.2023.10.012
Citation: LI J, ZHANG Q, ZHANG J X, et al. Effects of Straw-returning on Phosphorus Morphology and Microbial Phosphorus-cycling Genes in Rice Paddy Soil [J]. Fujian Journal of Agricultural Sciences,2023,38(10):1230−1241 doi: 10.19303/j.issn.1008-0384.2023.10.012

秸秆还田对稻田土壤磷素形态及磷循环微生物功能基因的影响

doi: 10.19303/j.issn.1008-0384.2023.10.012
基金项目: 广东省农业科学院低碳农业与碳中和研究中心项目(XT202220);广东省科技计划项目(2021B1212050020);广东省农业科学院农业优势产业学科团队建设项目(202121TD);广东省现代农业产业技术体系创新团队项目(2023KJ118);广东省农业科学院中青年学科带头人培养计划(R2020PY-JG012);广东省农业科学院汕尾分院科技合作专项(2021-分院专项-02);茂名实验室科研启动项目(2021TDQD002)
详细信息
    作者简介:

    李季(1999 —),女,硕士,主要从事土壤磷循环研究,E-mail:liji03162020@163.com

    通讯作者:

    解开治(1977 —),男,博士,研究员,主要从事农业微生物研究,E-mail:xiekzgsau@163.com

    徐培智(1963 —),男,学士,研究员,主要从事土壤碳氮磷循环研究,E-mail:pzxu007@163.com

  • 中图分类号: S153

Effects of Straw-returning on Phosphorus Morphology and Microbial Phosphorus-cycling Genes in Rice Paddy Soil

  • 摘要:   目的  以南方酸性土壤连续8年秸秆还田定位试验田为研究对象,探究秸秆还田对水稻分蘖期与成熟期土壤磷循环微生物功能基因的影响。  方法  定位试验共设置5个处理:化肥+无秸秆还田(CK)、化肥+当季秸秆全量还田(CKS)、化肥+当季秸秆全量还田+秸秆替代10%钾肥(S10)、化肥+当季秸秆全量还田+秸秆替代20%钾肥(S20)和化肥+当季秸秆全量还田+秸秆替代30%钾肥(S30)。利用Guppy土壤磷素连续浸提法和宏基因组测序技术,分别测定土壤磷素组分和磷循环微生物功能基因数据。  结果  秸秆还田处理显著提高了Guppy土壤磷分级中可利用磷组分NaHCO3-Pi的含量(P<0.05),其中S10、S20处理较CK处理提升了5.88%~8.73%;中等可利用磷组分中NaOH-Pi为南方酸性稻田土壤最主要的磷素形态,含量为154.03~202.11 mg·kg−1。同时,秸秆还田还显著影响了土壤磷循环微生物功能基因的丰度,其中无机磷溶解基因pqqC对秸秆全量还田处理的响应更为明显。秸秆还田条件下磷循环功能基因(如phnWphnOpqqBpqqC)促进了盐酸磷和残余磷向可利用磷库的活化,appAphnXppx基因参与了稳定态磷的活化过程,NaOH-Pi为关键的过渡态磷素,参与长期的磷素转化过程。此外,土壤有机碳和pH是决定功能基因丰度的主要影响因子。  结论  秸秆还田通过影响土壤理化性质,改变了稻田土壤磷循环功能基因丰度,促进了南方酸性稻田土壤磷素转化过程。
  • 图  1  水稻不同生育期不同处理土壤可利用磷组分及含量

    图中不同小写字母表示处理间差异显著(P<0.05)。下同。

    Figure  1.  Easily available phosphorus in soil under various treatments at rice growth stages

    Data with different lowercase letters indicate significant differences at P<0.05. Same for below.

    图  2  水稻不同生育期不同处理土壤中等可利用磷组分及含量

    Figure  2.  Moderately available phosphorus in soil under various treatments at rice growth stages

    图  3  水稻不同生育期不同处理土壤稳定态磷组分及含量

    Figure  3.  Non-available phosphorus in soil under various treatments at rice growth stages

    图  4  土壤有机磷矿化基因的相对丰度

    A和B分别代表水稻分蘖期与成熟期编码酸性磷酸酶、碱性磷酸酶和植酸酶的基因,C和D分别表示水稻分蘖期与成熟期编码C-P键裂解酶的基因。*( P <0.05),**( P <0.01)。

    Figure  4.  Relative abundance of organic phosphorus-mineralizing genes in soil

    A and B represent the genes encoding acid phosphatase, alkaline phosphatase and phytase at tillering and maturing stages, C and D represent the genes encoding C-P bond cleavage enzymes at tillering and maturing stages, respectively. * (P <0.05), ** (P <0.01).

    图  5  土壤无机磷溶解基因的相对丰度

    A和B分别表示水稻分蘖期和成熟期,下同。

    Figure  5.  Relative abundance of organic phosphorus-mineralizing genes in soil

    A and B represent tillering and maturing stages, same for below.

    图  6  土壤理化性质与磷循环功能基因的相关性分析

    Figure  6.  Correlation between physiochemical properties and phosphorus-cycling genes in soil

    图  7  土壤磷素组分与磷循环功能基因的相关性分析

    Figure  7.  Correlation between phosphorus components and phosphorus-cycling genes in soil

    表  1  不同处理化肥养分投入量

    Table  1.   Nutrients in treatment fertilizers (kg·hm−2

    处理
    Treatment
    早稻 Early rice 晚稻 Late rice 总计 Total
    NP2O5K2O NP2O5K2O NP2O5K2O
    CK 155.3 47.3 135.0 155.3 47.3 135.0 310.5 94.5 270.0
    CKS 155.3 47.3 135.0 155.3 47.3 135.0 310.5 94.5 270.0
    S10 155.3 47.3 121.5 155.3 47.3 121.5 310.5 94.5 243.0
    S20 155.3 47.3 108.0 155.3 47.3 108.0 310.5 94.5 216.0
    S30 155.3 47.3 94.5 155.3 47.3 94.5 310.5 94.5 189.0
    下载: 导出CSV

    表  2  磷循环微生物功能基因

    Table  2.   Microbial phosphorus-cycling genes

    功能基因分组
    Functional gene grouping
    基因名称
    Gene name
    KEGG编号
    KEGG number
    基因功能详情
    Gene Function Detials
    有机磷矿化基因
    Genes involved in organic P-mineralization
    phoNK09474酸性磷酸酶 (A) Acid phosphatase (class A)
    olpAK01078酸性磷酸酶(C)acid phosphatase (class C)
    aphAK03788酸性磷酸酶 (B) Acid phosphatase (class B)
    phoAK01077碱性磷酸酶 Alkaline phosphatase
    phoDK01113碱性磷酸酶 Alkaline phosphatase D
    phoXK02040碱性磷酸酶 Alkaline phosphatase
    phyAK010833-植酸酶 3-phytase
    appAK010934-植酸酶/酸性磷酸酶 4-phytase/acid phosphatase
    phnWK034302-氨基乙基膦酸-丙酮酸转氨酶 2-aminoethylphosphonate-pyruvate transaminase
    phnXK05306膦酸乙醛水解酶 Phosphonoacetaldehyde hydrolase
    phnAK06193烷基膦酸酯利用操纵子 Alkylphosphonate utilization operon protein
    phnNK05774碳-磷键裂解酶复合物 C-P lyase multienzyme complex
    phnLK05780碳-磷键裂解酶复合物 C-P lyase multienzyme complex
    phnMK06162碳-磷键裂解酶复合物 C-P lyase multienzyme complex
    phnFK02043酸酯转运系统调控蛋白 Phosphonate transport system regulatory protein
    phnGK06166碳-磷键裂解酶复合物 C-P lyase multienzyme complex
    phnHK06165碳-磷键裂解酶复合物 C-P lyase multienzyme complex
    phnIK06164碳-磷键裂解酶复合物 C-P lyase multienzyme complex
    phnJK06163碳-磷键裂解酶复合物 C-P lyase multienzyme complex
    phnOK09994碳-磷键裂解酶复合物 C-P lyase multienzyme complex
    phnKK05781C-P 裂解酶亚基,α-d-核糖 1-甲基膦酸 5-三磷酸合酶
    C-P lyase subunit,alpha-D-ribose 1-methylphosphonate 5-triphosphate synthase
    phnPK06167酸核糖基1,2-环磷酸二酯酶 Phosphoribosyl 1,2-cyclicphosphate phosphoDiesterase
    无机磷溶解基因
    Inorganic phosphate solubilization
    ppaK01507无机焦磷酸酶 Inorganic pyrophosphatase
    ppxK01524外切多聚磷酸酶 Exopolyphosphatase/鸟苷-5'-三磷酸,3'-二磷酸焦磷酸酶
    Guanosine-5'-triphosphate 3'-diphosphate pyrophosphatase
    ppkK00937多聚磷酸激酶 Polyphosphate kinase
    gcdK00117葡萄糖脱氢酶 Quinoprotein glucose dehydrogenase
    pqqBK06136吡咯喹啉醌合成蛋白 B Pyrroloquinoline quinonebiosynthesis protein B
    pqqCK06137吡咯喹啉醌合成酶 Pyrroloquinoline-quinone synthase
    pqqDK06138吡咯喹啉醌合成蛋白 Pyrroloquinoline quinonebiosynthesis protein
    pqqEK06139PqqA 肽环酶 PqqA peptide cyclase
    下载: 导出CSV
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出版历程
  • 收稿日期:  2023-04-10
  • 录用日期:  2023-04-10
  • 修回日期:  2023-05-17
  • 网络出版日期:  2023-10-25
  • 刊出日期:  2023-10-28

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