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外源褪黑素对低温逆境下三角梅生理特性和细胞壁组分的影响

胡牮 林雅楠 吴楠 蒋诗睿 宋杨 张建新 郑玲霞 陈祺月 刘鹏

胡牮,林雅楠,吴楠,等. 外源褪黑素对低温逆境下三角梅生理特性和细胞壁组分的影响 [J]. 福建农业学报,2024,39(X):1−9
引用本文: 胡牮,林雅楠,吴楠,等. 外源褪黑素对低温逆境下三角梅生理特性和细胞壁组分的影响 [J]. 福建农业学报,2024,39(X):1−9
HU J, LIN Y N, WU N, et al. Effects of Exogenous Melatonin on Physiological Characteristics and Cell Wall Components of Bougainvillea Under Cold Stress [J]. Fujian Journal of Agricultural Sciences,2024,39(X):1−9
Citation: HU J, LIN Y N, WU N, et al. Effects of Exogenous Melatonin on Physiological Characteristics and Cell Wall Components of Bougainvillea Under Cold Stress [J]. Fujian Journal of Agricultural Sciences,2024,39(X):1−9

外源褪黑素对低温逆境下三角梅生理特性和细胞壁组分的影响

基金项目: 国家自然科学基金项目(32001224,30540056); 浙江省公益研究计划项目(LGN19C140009);浙江大花园建设研究院2021年专项课题(DHYB1001)
详细信息
    作者简介:

    胡牮(1985 —),男,硕士,副教授,主要从事植物逆境生理、园林植物及其应用研究,E-mail:hudainiu@163.com

    通讯作者:

    刘鹏(1965−),男,博士,教授,主要从事植物逆境生理、环境污染与保护及植物生理生态研究,E-mail:sky79@zjnu.cn

  • 中图分类号: S685.99

Effects of Exogenous Melatonin on Physiological Characteristics and Cell Wall Components of Bougainvillea Under Cold Stress

  • 摘要:   目的  研究褪黑素对低温逆境下三角梅生理生化及细胞壁组分上的调控作用,为缓解低温对三角梅生长造成的不利影响提供理论依据。  方法  以耐冷性不同的三角梅安格斯、口红、变色龙3个品种为供试材料,在4 ℃低温下对三角梅植株叶面施加不同浓度(0、50、100、150 µmol·L–1)的外源褪黑素,测定各处理组生长周期(0、7、14、21 d)过程中三角梅叶面积、叶绿素含量、叶绿素荧光参数[PSⅡ最大光化学量子产量(Fv/Fm)、表观电子传递速率(ETR)、光化学淬灭系数(qP)、非光化学淬灭系数(qN)]、抗氧化酶[超氧化物歧化酶(SOD)、过氧化物酶(POD)和过氧化氢酶(CAT)]活性、膜脂过氧化产物[超氧阴离子(O2)产生速率、过氧化氢(H2O2)含量]、渗透调节物质[可溶性糖(SS)、可溶性蛋白(SP)]含量、细胞壁组分[螯合型果胶(CSP)、碱溶性果胶(SSP)、半纤维素(HC)和纤维素(CE)]含量。  结果  低温逆境下三角梅的正常生长发育受阻,叶绿素荧光参数失衡,膜脂过氧化加剧,细胞壁组分受到影响。而施加100 µmol·L–1的褪黑素能够有效地提升三角梅的生理活性,改善植株萎蔫程度,Fv/FmETR降幅减小;抗氧化酶活性显著增加,安格斯较于0 d的SOD、POD、CAT最大可增加96.45%、104.35%、73.11%;H2O2含量、O2生成速率对比同周期MT0最多下降21.07%、26.85%;细胞壁组分中CSP、SSP上升幅度增加,较于0 d最多可上涨22.55%、43.08%。  结论  明确了外源褪黑素在生理特性方面对不同品种三角梅冷害的调控作用,外源施加适宜浓度的褪黑素可以有效缓解低温对三角梅生长的抑制,减轻光系统损伤,增强抗氧化酶活性,减缓膜脂氧化,并调节细胞壁各组分含量以维持其结构整体稳定,从而降低低温伤害,为探索褪黑素对冷害下植物的调控作用提供新思路。
  • 图  1  MT处理对低温下三角梅表观性状的影响

    Figure  1.  Effects of MT treatment on apparent character of Bougainvillea at low temperature

    EA:Bougainvillea Elizabeth Angus;SC:Bougainvillea Snow Cap;MH:Bougainvillea Marry Helen,as below.

    图  2  MT处理对低温下三角梅叶绿素含量的影响

    不同小写字母表示同一品种不同处理间差异显著(P<0.05),下同。

    Figure  2.  Effects of MT treatment on chlorophyll content of Bougainvillea at low temperature

    Data with different lowercase indicate significant differences between treatments on same Bougainvillea variety at P<0.05,as below.、

    图  3  MT处理对低温下三角梅叶绿素荧光参数的影响

    Figure  3.  Effect of MT treatment on on chlorophyll fluorescence parameters of Bougainvillea under low temperature

    图  4  MT处理对低温下三角梅过氧化氢含量和超氧阴离子产生速率的影响

    Figure  4.  Effect of MT treatment on hydrogen peroxide content and superoxide anion production rate of Bougainvillea under low temperature

    图  5  MT处理对低温下三角梅抗氧化酶系统的影响

    Figure  5.  Effect of MT treatment on antioxidant oxidase system of Bougainvillea under low temperature

    图  6  MT处理对低温下三角梅可溶性糖和可溶性蛋白含量的影响

    Figure  6.  Effect of MT treatment on contents of soluble sugar and soluble protein in Bougainvillea under low temperature

    图  7  MT处理对低温下三角梅细胞壁组分的影响

    Figure  7.  Effect of MT treatment on cell wall components of Bougainvillea under low temperature

  • [1] QIN B, SHI Y S, TANG Q, et al. Study on the industry status of Chinese Bougainvillea and its popularization strategy in Guangxi [J]. Agricultural Research and Application, 2020, 33(6): 56−60.
    [2] 张昭, 聂宇婷, 崔凯伦, 等. 褪黑素调控草类植物生长发育及抗逆性功能研究进展[J]. 草地学报, 2581. (in Chinese

    3, 31(9): 2571-2581. ZHANG Z, NIE Y T, CUI K L, et al. Research progress on the function of melatonin in regulating growth, development and stress resistance in herbaceous species[J]. Acta Agrestia Sinica, 2023, 31(9): 2571-
    [3] SHI H T, CHEN Y H, TAN D X, et al. Melatonin induces nitric oxide and the potential mechanisms relate to innate immunity against bacterial pathogen infection in Arabidopsis [J]. Journal of Pineal Research, 2015, 59(1): 102−108. doi: 10.1111/jpi.12244
    [4] 蒋朝维, 吴俊杰, 姜浩, 等. 低温胁迫下烤烟幼苗对喷施外源褪黑素的生理响应 [J]. 江苏农业科学, 2023, 51(21):62−66.

    JIANG C W, WU J J, JIANG H, et al. Physiological response of flue-cured tobacco seedlings to exogenous melatonin spray under low temperature stress [J]. Jiangsu Agricultural Sciences, 2023, 51(21): 62−66. (in Chinese)
    [5] IMRAN M, LATIF KHAN A, SHAHZAD R, et al. Exogenous melatonin induces drought stress tolerance by promoting plant growth and antioxidant defence system of soybean plants [J]. AoB PLANTS, 2021, 13(4): plab026. doi: 10.1093/aobpla/plab026
    [6] MICHARD E, SIMON A A. Melatonin’s antioxidant properties protect plants under salt stress[J]. Plant, Cell & Environment, 2020, 43(11): 2587-2590.
    [7] LIU N, LI J W, LV J, et al. Melatonin alleviates imidacloprid phytotoxicity to cucumber (Cucumis sativus L. ) through modulating redox homeostasis in plants and promoting its metabolism by enhancing glutathione dependent detoxification [J]. Ecotoxicology and Environmental Safety, 2021, 217: 112248. doi: 10.1016/j.ecoenv.2021.112248
    [8] 孙志娟, 刘文杰, 郑晓东, 等. 褪黑素对盐碱胁迫下平邑甜茶幼苗生长的影响及其机制 [J]. 园艺学报, 2023, 50(8):1697−1710.

    SUN Z J, LIU W J, ZHENG X D, et al. Effects and functional mechanism of melatonin on the growth of Malus hupehensis seedlings under saline-alkali stress [J]. Acta Horticulturae Sinica, 2023, 50(8): 1697−1710. (in Chinese)
    [9] 任利明, 谢芳, 丁飞, 等. 褪黑素对花椒幼苗低温伤害的缓解效应 [J]. 西北林学院学报, 2023, 38(4):34−43. doi: 10.3969/j.issn.1001-7461.2023.04.05

    REN L M, XIE F, DING F, et al. The mitigative effect of melatonin on Zanthoxylum bungeanum seedlings under chilling stress [J]. Journal of Northwest Forestry University, 2023, 38(4): 34−43. (in Chinese) doi: 10.3969/j.issn.1001-7461.2023.04.05
    [10] 李方一, 黄璜, 官春云. 作物叶面积测量的研究进展 [J]. 湖南农业大学学报(自然科学版), 2021, 47(3):274−282.

    LI F Y, HUANG H, GUAN C Y. Review on measurement of crop leaf area [J]. Journal of Hunan Agricultural University (Natural Sciences), 2021, 47(3): 274−282. (in Chinese)
    [11] SHIBAEVA T G, MAMAEV A V, SHERUDILO E G. Evaluation of a SPAD-502 plus chlorophyll meter to estimate chlorophyll content in leaves with interveinal chlorosis [J]. Russian Journal of Plant Physiology, 2020, 67(4): 690−696. doi: 10.1134/S1021443720040160
    [12] 努尔凯麦尔·木拉提, 杨亚杰, 帕尔哈提·阿布都克日木, 等. 小麦叶绿素含量测定方法比较 [J]. 江苏农业科学, 2021, 49(9):156−159.

    NURKHEIMER M, YANG Y J, PARHATI A, et al. Comparative study on determination methods of chlorophyll content in wheat [J]. Jiangsu Agricultural Sciences, 2021, 49(9): 156−159. (in Chinese)
    [13] SEPEHRI E, HOSSEINI B, HEDAYATI A. The effect of iron oxide Nano-Particles on the production of tropane alkaloids, h6h gene expression and antioxidant enzyme activity in Atropa belladonna hairy roots [J]. Russian Journal of Plant Physiology, 2022, 69(6): 122. doi: 10.1134/S1021443722060243
    [14] TIMINS, PENG J, YU Z. Exogenous glycine betaine treatment enhances chilling tolerance of peach fruit during cold storage[J]. Postharvest Biology and Technology, 2016: 104-110.
    [15] 董扬. 低温胁迫对不同耐冷性糜子品种苗期耐冷性的影响 [J]. 作物杂志, 2023, 39(6):1−7

    DONG Y. Effects of low temperature stress on cold tolerance of broomcorn millet varieties with different cold tolerance at seedling stage [J]. Crop Journal, 2023, 39(6): 1−7(in Chinese)
    [16] 张治安, 陈展宇. 植物生理学实验技术[M]. 长春: 吉林大学出版社, 2008.

    ZHANG Z A, CHEN Z Y. Experimental technology of plant physiology[M]. Changchun: Jilin University Press, 2008. (in Chinese)
    [17] 沈蔡慰. 硼对铝胁迫下栝楼有机酸及细胞壁成分调控作用的探究[D]. 金华: 浙江师范大学, 2020

    SHEN C W. Effects of Boron on Organic Acids and Cell Wall Components of Trichosanthes Kirilowii Maxim. Under Aluminum Stress[D]. Jinhua: Zhejiang Normal University, 2020. (in Chinese)
    [18] DAT J, VANDENABEELE S, VRANOVÁ E, et al. Dual action of the active oxygen species during plant stress responses [J]. Cellular and Molecular Life Sciences: CMLS, 2000, 57(5): 779−795. doi: 10.1007/s000180050041
    [19] 王荣荣, 王海琪, 蒋桂英, 等. 2个不同抗旱性小麦品种耗水特征及根系生理特性对开花期干旱的响应 [J]. 水土保持学报, 2022, 36(4):253−264.

    WANG R R, WANG H Q, JIANG G Y, et al. Response of water consumption and root physiological characteristics of two different drought-tolerant wheat varieties to anthesis stage drought [J]. Journal of Soil and Water Conservation, 2022, 36(4): 253−264. (in Chinese)
    [20] 李欠敏, 杨琳, 段巧红, 等. 外源褪黑素对低温胁迫下大白菜幼苗生长及生理特性的影响 [J]. 山东农业大学学报(自然科学版), 2021, 52(2):182−186.

    LI Q M, YANG L, DUAN Q H, et al. Effects of exogenous melatonin on the growth and physiological characteristics of Chinese cabbage seedlings under chilling stress [J]. Journal of Shandong Agricultural University (Natural Science Edition), 2021, 52(2): 182−186. (in Chinese)
    [21] 尤鑫, 龚吉蕊. 叶绿素荧光动力学参数的意义及实例辨析 [J]. 西部林业科学, 2012, 41(5):90−94. doi: 10.3969/j.issn.1672-8246.2012.05.017

    YOU X, GONG J R. Significance and application of chlorophyll fluorescence dynamics process parameters [J]. Journal of West China Forestry Science, 2012, 41(5): 90−94. (in Chinese) doi: 10.3969/j.issn.1672-8246.2012.05.017
    [22] 丁东霞, 李能慧, 李静, 等. 外源褪黑素对低温弱光胁迫下辣椒叶绿素荧光和抗氧化系统的影响 [J]. 浙江农业学报, 2022, 34(9):1935−1944. doi: 10.3969/j.issn.1004-1524.2022.09.12

    DING D X, LI N H, LI J, et al. Effects of exogenous melatonin on chlorophyll fluorescence and antioxidant system of pepper (Capsicum annuum L.) under low temperature and low light stress [J]. Acta Agriculturae Zhejiangensis, 2022, 34(9): 1935−1944. (in Chinese) doi: 10.3969/j.issn.1004-1524.2022.09.12
    [23] 朱晓丹. 不同乌菜品种在低温胁迫下的生理响应[D]. 合肥: 安徽农业大学, 2017.

    ZHU X D. Physiological Response of Different Cultivars of under Low Temperature Stress in Brassica Campestris L. ssp. Chinese(L. ) Makino Var Rosularis Tsen Et Lee[D]. Hefei: Anhui Agricultural University, 2017. (in Chinese)
    [24] AYYAZ A, FAROOQ M A, DAWOOD M, et al. Exogenous melatonin regulates chromium stress-induced feedback inhibition of photosynthesis and antioxidative protection in Brassica napus cultivars [J]. Plant Cell Reports, 2021, 40(11): 2063−2080. doi: 10.1007/s00299-021-02769-3
    [25] 白如意, 宋希梅, 沈健, 等. 叶面喷施褪黑素对低温胁迫下南瓜幼苗生长和生理特性的影响 [J]. 西北植物学报, 2023, 43(5):805−813. doi: 10.7606/j.issn.1000-4025.2023.05.0805

    BAI R Y, SONG X M, SHEN J, et al. Effect of foliar spraying melatonin on growth and physiological characteristics of pumpkin seedlings under cold stress [J]. Acta Botanica Boreali-Occidentalia Sinica, 2023, 43(5): 805−813. (in Chinese) doi: 10.7606/j.issn.1000-4025.2023.05.0805
    [26] SALADIN G, CLÉMENT C, MAGNÉ C. Stress effects of flumioxazin herbicide on grapevine (Vitis vinifera L. ) grown in vitro [J]. Plant Cell Reports, 2003, 21(12): 1221−1227. doi: 10.1007/s00299-003-0658-x
    [27] 唐鸿吕. 外源褪黑素对低温胁迫下甘蓝苗期生长、生理特性及成株产量的影响[D]. 杨凌: 西北农林科技大学, 2022

    TANG H L. Effects of Exogenous Melatonin on Seedling Growth Physiological Characteristics and Yield of Cabbage under Low Temperature Stress[D]. Yangling: Northwest A & F University, 2022. (in Chinese)
    [28] 宋静蕾, 潘晓帆, 裴家伟, 等. 红薯渣中不同果胶组分的提取及结构表征 [J]. 中国调味品, 2023, 48(8):24−32. doi: 10.3969/j.issn.1000-9973.2023.08.005

    SONG J L, PAN X F, PEI J W, et al. Extraction and structural characterization of different pectin components from sweet potato pomace [J]. China Condiment, 2023, 48(8): 24−32. (in Chinese) doi: 10.3969/j.issn.1000-9973.2023.08.005
    [29] 陈立莉, 陈小爱, 徐飞, 等. 不同溶解性面包果果胶的理化、结构性质及抗氧化活性的比较 [J]. 现代食品科技, 2023, 22(5):168−172

    CHEN L L, CHEN X A, XU F, et al. Physicochemical, structural properties and antioxidant activity of breadfruit pectin with different solubility [J]. Modern Food Science and Technology, 2023, 22(5): 168−172. (in Chinese)
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  • 收稿日期:  2024-01-04
  • 修回日期:  2024-03-07
  • 网络出版日期:  2024-05-08

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