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蚕豆芽苗菜高左旋多巴产量的LED光培养条件研究

李程勋 徐晓俞 李爱萍 郑开斌

李程勋,徐晓俞,李爱萍,等. 蚕豆芽苗菜高左旋多巴产量的LED光培养条件研究 [J]. 福建农业学报,2023,38(5):545−551 doi: 10.19303/j.issn.1008-0384.2023.05.005
引用本文: 李程勋,徐晓俞,李爱萍,等. 蚕豆芽苗菜高左旋多巴产量的LED光培养条件研究 [J]. 福建农业学报,2023,38(5):545−551 doi: 10.19303/j.issn.1008-0384.2023.05.005
LI C X, XU X Y, LI A P, et al. LED-stimulated Levodopa Production in Broad Bean Sprouts [J]. Fujian Journal of Agricultural Sciences,2023,38(5):545−551 doi: 10.19303/j.issn.1008-0384.2023.05.005
Citation: LI C X, XU X Y, LI A P, et al. LED-stimulated Levodopa Production in Broad Bean Sprouts [J]. Fujian Journal of Agricultural Sciences,2023,38(5):545−551 doi: 10.19303/j.issn.1008-0384.2023.05.005

蚕豆芽苗菜高左旋多巴产量的LED光培养条件研究

doi: 10.19303/j.issn.1008-0384.2023.05.005
基金项目: 福建省公益类科研院所基本科研专项(2021R1031004);福建省农业种质资源创新专项(2023);福建省农业科学院科技创新团队(CXTD2021011-2);福建省农业高质量发展超越“5511”协同创新工程项目(XTCXGC2021019)
详细信息
    作者简介:

    李程勋(1991−),男,硕士,助理研究员,研究方向:作物遗传品质育种与农产品天然产物提取(Email:1219513539@qq.com

    通讯作者:

    李爱萍(1968−),女,研究员,研究方向:作物遗传品质育种及农产品天然产物提取(E-mail:apl909@163.com

    郑开斌(1966−),男,博士,研究员,研究方向:作物遗传品质育种及农产品天然产物提取(E-mail:k03163@163.com

  • 中图分类号: S 529

LED-stimulated Levodopa Production in Broad Bean Sprouts

  • 摘要:   目的  研究不同LED光培养条件对蚕豆芽苗菜功效成分左旋多巴产量的影响。  方法  通过单因素试验和L9(34)正交试验研究不同光强、光照时间和培养时间3个因素对蚕豆芽苗菜生长及其功效物质左旋多巴产量的影响。  结果  弱光促进蚕豆芽苗菜干物质的积累,强光促进蚕豆芽苗菜左旋多巴质量分数的积累,蚕豆芽苗菜高左旋多巴产量的最优培养条件为光强500 lx、光照时间9 h、培养时间6 d,在此培养条件下蚕豆芽苗菜左旋多巴产量为19.62 g·m−2  结论  通过调节蚕豆芽苗菜的光培养条件可以提高其功效物质左旋多巴的产量,本研究为蚕豆芽苗菜的功效物质成分提取提供参考依据,有利于蚕豆芽苗菜的开发利用。
  • 图  1  培养时间对蚕豆芽苗菜的影响

    Figure  1.  Effect of culture time on broad bean sprouts

    图  2  光强对蚕豆芽苗菜的影响

    Figure  2.  Effect of LED light intensity on broad bean sprouts

    图  3  光照时间对蚕豆芽苗菜的影响

    Figure  3.  Effect of LED exposure time on broad bean sprouts

    表  1  因素水平

    Table  1.   Factors and levels of orthogonal experiment

    水平
    Level
    因素 Factor
    A培养时间
    Culture time/d
    B 光强
    Light intensity/lx
    C 光照时间
    Light time/h
    162506
    275009
    3875012
    下载: 导出CSV

    表  2  蚕豆芽苗菜左旋多巴产量的正交试验结果与分析表

    Table  2.   Orthogonal test results and analysis on levodopa produced in broad bean sprouts

    编号
    Number
    因素 Factor左旋多巴产量
    Levodopa yield/(g·m−2
    A培养时间
    Culture time
    B光强
    Light intensity
    C光照时间
    Light time
    空列
    Empty column
    1 1 1 1 1 16.83
    2 1 2 2 2 19.62
    3 1 3 3 3 17.56
    4 2 1 2 3 19.26
    5 2 2 3 1 19.55
    6 2 3 1 2 16.87
    7 3 1 3 2 18.64
    8 3 2 1 3 18.64
    9 3 3 2 1 18.06
    K1 54.01 54.73 52.34 54.44
    K2 55.68 57.81 56.94 55.13
    K3 55.34 52.49 55.75 55.46
    k1 18.00 18.24 17.45 18.15
    k2 18.56 19.27 18.98 18.38
    k3 18.45 17.50 18.58 18.49
    极差 Range 0.56 1.77 1.53 0.34
    因素主次 Factor primary and secondary B>C>A
    优水平 Excellent level A2 B2 C2
    优组合 Excellent combination B2C2A2
    下载: 导出CSV

    表  3  方差分析

    Table  3.   Analysis of variance

    因素
    Factor
    偏差平方和
    Sum of squares of
    deviations
    自由度
    Degree of
    freedom
    F
    F value
    显著性
    Significance
    A0.5222.89
    B4.75226.37*
    C3.80221.10*
    F0.05(2,2)=19,当F>19说明有显著性意义,*P<0.05。
    F0.05(2,2)=19, F >19 means significant difference (P<0.05).
    下载: 导出CSV
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  • 收稿日期:  2021-11-17
  • 录用日期:  2022-08-03
  • 修回日期:  2022-04-20
  • 网络出版日期:  2023-03-06
  • 刊出日期:  2023-05-28

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