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茶树炭疽菌N425全基因组测序与基因功能注释

张承康 周子文 郭田龙 彭成彬 陈美霞 刘伟

张承康,周子文,郭田龙,等. 茶树炭疽菌N425全基因组测序与基因功能注释 [J]. 福建农业学报,2023,38(12):1437−1444 doi: 10.19303/j.issn.1008-0384.2023.12.007
引用本文: 张承康,周子文,郭田龙,等. 茶树炭疽菌N425全基因组测序与基因功能注释 [J]. 福建农业学报,2023,38(12):1437−1444 doi: 10.19303/j.issn.1008-0384.2023.12.007
ZHANG C K, ZHOU Z W, GUO T L, et al. Sequence and Annotation of Colletotrichum fructicola N425 Genome on Tea Plant [J]. Fujian Journal of Agricultural Sciences,2023,38(12):1437−1444 doi: 10.19303/j.issn.1008-0384.2023.12.007
Citation: ZHANG C K, ZHOU Z W, GUO T L, et al. Sequence and Annotation of Colletotrichum fructicola N425 Genome on Tea Plant [J]. Fujian Journal of Agricultural Sciences,2023,38(12):1437−1444 doi: 10.19303/j.issn.1008-0384.2023.12.007

茶树炭疽菌N425全基因组测序与基因功能注释

doi: 10.19303/j.issn.1008-0384.2023.12.007
基金项目: 国家自然科学基金项目(32001853);生物农药与化学生物学教育部重点实验室开放课题(Keylab2020-05);宁德师范学院科研项目(2020Y02、2022T01)
详细信息
    作者简介:

    张承康(1988 —),男,博士,讲师,主要从事植物病原菌致病分子机理研究,E-mail:shin_ichi@126.com

    通讯作者:

    刘伟(1971 — ),男,博士,研究员,主要从事植物病害防治研究,E-mail:liuwei0591@126.com

  • 中图分类号: S436

Sequence and Annotation of Colletotrichum fructicola N425 Genome on Tea Plant

  • 摘要:   目的  对茶树炭疽菌N425进行全基因组测序和基因功能注释并从中初筛出致病相关基因。  方法  通过Illumina HiSeq2500 PE150平台对N425菌株进行全基因组测序和组装,并利用NR、KEGG、KOG等功能基因数据库进行基因预测和功能注释。  结果  茶树炭疽菌N425基因组全长约56.3 Mbp,G+C含量为53.2%,共预测出10 157个蛋白编码基因和若干各类非蛋白编码序列。其中,1 356个基因在病原与宿主互作数据库(PHI)中得到注释,包括140个注释为致病力丧失的基因;720个基因在碳水化合物酶数据库(CAZy)中得到注释;302个基因被预测为次生代谢物相关基因。这些基因中有涉及cAMP-PKA、MAPK等信号通路以及降解宿主细胞壁等致病相关过程,是潜在的致病相关基因。  结论  本研究成功获得茶树炭疽菌N425全基因组序列和基因功能注释,并从中挖掘出潜在致病相关基因,为后续展开对茶树炭疽菌侵染茶树的致病分子机制研究奠定基础。
  • 图  1  茶树炭疽菌N425基因长度分布

    Figure  1.  Distribution of C. fructicola N425 gene length

    图  2  茶树炭疽菌N425基因在不同数据库中得到注释的个数

    Figure  2.  Number of annotated C. fructicola N425 genes from various databases

    图  3  在CAZy数据库中得到注释的茶树炭疽菌N425基因的分类

    Figure  3.  Classification of C. fructicola N425 genes annotated in CAZy database

    图  4  茶树炭疽菌N425中预测为次生代谢物相关的基因簇和基因分布

    Figure  4.  Distributions of predicted secondary metabolites-associated gene clusters and genes in C. fructicola N425

    表  1  经PHI数据库归类为致病性丧失的前18个基因的注释信息

    Table  1.   Annotation on first 18 loss of pathogenicity genes classified by PHI database

    基因编号
    Gene No.
    同源基因名称
    Homologous gene name
    同源基因所属病原菌
    Pathogen to which homologous gene belongs
    宿主
    Host
    基因注释
    Gene annotation
    参考文献
    Reference
    A00871 ChMK1 希金斯炭疽菌
    Colletotrichum higginsianum
    拟南芥
    Thale cress
    促分裂原活化蛋白激酶
    Mitogen-activated protein kinase(MAPK)
    [17]
    A09504 CgAP1 胶孢炭疽菌
    C. gloeosporioides
    芒果
    Mango
    bZIP 转录因子
    bZIP transcription factor
    [18]
    A08060 CAP20 胶孢炭疽菌
    C. gloeosporioides
    牛油果
    Avocado
    附着胞细胞壁
    Appressorial wall
    [19]
    A03691 CAM 稻瘟病菌
    Magnaporthe oryzae
    小麦
    Wheat
    钙调蛋白
    Calmodulin
    [20]
    A04430 MoYpt7 稻瘟病菌
    M. oryzae
    水稻
    Rice
    小GTP酶
    Small GTPase
    [21]
    A04622 ClaSSD1 葫芦炭疽菌
    C. lagenaria
    黄瓜
    Cucumber
    细胞壁组装调控
    Regulation of cell wall assembly
    [22]
    A09136 ChSte7 希金斯炭疽菌
    C. higginsianum
    拟南芥
    Thale cress
    促分裂原活化蛋白激酶
    Mitogen-activated protein kinase kinase(MAPKK)
    [23]
    A01252 RAC1 稻瘟病菌
    M. oryzae
    水稻
    Rice
    RhoGTP酶
    Rho GTPase
    [24]
    A00884 CNB 稻瘟病菌
    M. oryzae
    小麦
    Wheat
    钙调磷酸酶调节亚基
    Calcineurin regulatory subunits
    [20]
    A03506 CLAP1 菜豆炭疽病菌
    C. lindemuthianum
    四季豆
    String bean
    铜转运ATP酶
    Copper transporting ATPase
    [25]
    A02922 ChPMA2 希金斯炭疽菌
    C. higginsianum
    拟南芥
    Thale cress
    质膜质子泵
    Plasma membrane proton pump
    [26]
    A09008 GPI8 禾生炭疽菌
    C. graminicola
    玉米
    Maize
    半胱氨酸蛋白酶
    Cystein protease
    [27]
    A06226 CgPKS1 禾生炭疽菌
    C. graminicola
    玉米
    Maize
    聚酮合酶
    Polyketide synthase
    [28]
    A02402 ACL 禾谷镰刀菌
    Fusarium graminearum
    小麦
    Wheat
    ATP柠檬酸裂解酶
    ATP citrate lyase
    [29]
    A09126 RPK1 葫芦炭疽菌
    C. lagenaria
    黄瓜
    Cucumber
    蛋白激酶A调节亚基
    Protein kinase A(PKA)regulatory subunit
    [30]
    A05844 FGA2 尖孢镰刀菌
    F. oxysporum
    西红柿
    Tomato
    G蛋白α亚基
    G protein alpha subunit
    [31]
    A01313 MgRho3 稻瘟病菌
    M. oryzae
    水稻
    Rice
    RhoGTP酶
    Rho GTPase
    [32]
    A07576 VdNoxB 大丽轮枝菌
    Verticillium dahliae
    棉花
    Cotton
    NADPH 氧化酶
    NADPH oxidase
    [33]
    下载: 导出CSV

    表  2  部分CAZy基因的注释信息

    Table  2.   Annotation on some CAZy genes

    基因编号
    Gene No.
    同源基因GenBank登录号
    Homologous gene
    GenBank accession No.
    同源基因所属病原菌
    Pathogen to which homologous gene belongs
    基因注释
    Gene annotation
    CAZy_家族
    CAZy_family
    A02429 AUT30986.1 胶孢炭疽菌 Colletotrichum gloeosporioides 果胶裂解酶2 Pectin lyase 2 PL1_4
    A03941 ELA25906.1 果生炭疽菌 C. fructicola 半乳糖氧化酶前体 Galactose oxidase precursor AA5_2
    A03990 AUT30987.1 胶孢炭疽菌 C. gloeosporioides 多聚半乳糖醛酸内切酶1 Endopolygalacturonase 1 GH28
    A07825 AUT30984.1 胶孢炭疽菌 C. gloeosporioides 果胶酸裂解酶B Pectate lyase B PL1_7
    A00270 AHC30245.1 胶孢炭疽菌 C. gloeosporioides 漆酶2 Laccase 2 AA1_3
    A00454 AND80748.1 胶孢炭疽菌 C. gloeosporioides 内切葡聚糖酶1B Endoglucanase 1B GH12
    A07600 AAL38030.1 胶孢炭疽菌 C. gloeosporioides 角质酶 Cutinase CE5
    A06012 AFK76451.1 胶孢炭疽菌 C. gloeosporioides 漆酶 Laccase AA1_3
    A07644 AAD00894.3 胶孢炭疽菌 C. gloeosporioides 甾醇糖基转移酶 Sterol glycosyltransferase GT1
    A07350 AUT30983.1 胶孢炭疽菌 C. gloeosporioides 果胶酸裂解酶A Pectate lyase A PL1_7
    A04870 AUT30985.1 胶孢炭疽菌 C. gloeosporioides 果胶裂解酶1 Pectin lyase 1 CBM1/PL1_4
    A02466 AAG32629.1 里氏木霉 Trichoderma reesei 甘露糖磷酸醇合成酶 Mannose phospho-dolichol synthase GT2
    A04591 CAP73770.1 柄孢霉 Podospora anserina 糖原合成酶 Glycogen synthase GT3
    A08316 AAL23719.1 禾生炭疽菌 C. graminicola 几丁质合成酶C Chitin synthase C GT2
    A00674 AAL23718.1 禾生炭疽菌 C. graminicola 几丁质合成酶B Chitin synthase B GT2
    A06213 AAL23717.1 禾生炭疽菌 C. graminicola 几丁质合成酶A Chitin synthase A GT2
    下载: 导出CSV
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出版历程
  • 收稿日期:  2023-08-29
  • 修回日期:  2023-09-30
  • 网络出版日期:  2023-12-21
  • 刊出日期:  2023-12-28

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