摘要:
比较4种不同极性大孔树脂对胭脂李果实多酚的静态吸附和解吸性能,筛选最佳纯化树脂;优化动态吸附与解吸条件参数,分析纯化前后多酚的黄嘌呤氧化酶抑制活性。结果表明:最佳纯化大孔树脂为HP-20,其静态吸附6 h达到饱和,静态解吸2 h后基本达到平衡;动态纯化最佳工艺条件为上样浓度1.0 mgmL-1,上样速度2.0 mLmin-1,80%乙醇溶液作为洗脱剂,洗脱流速2.0 mLmin-1;纯化后胭脂李多酚纯度由25.47%提高至74.89%,黄嘌呤氧化酶抑制活性IC50从179.21 gmL-1降到72.35 gmL-1,黄嘌呤氧化酶抑制活性提高了2.47倍。
关键词:
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胭脂李 /
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多酚 /
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大孔树脂 /
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纯化 /
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黄嘌呤氧化酶
Abstract:
Staticadsorption and desorption of 4 macroporous resins were evaluated for the purification of polyphenols from pruns, Prunus salicina Lindl. The dynamic conditions for the filtration process were optimized using theinhibitory activity of the filtered polyphenols on xanthine oxidase as a yardstick.As a result, HP-20 was chosen as the resin for the filtration. HP-20 had asaturation time of 6 h under static adsorption,and anequilibrium timeof 2 h for thedesorption. On the filtration process, the optimized conditions were determined to includeafeeding solution atthe concentration of 1.0 mgmL-1, a solution loadingrate at 2.0 mLmin-1, and the use of 80% ethanol for elution at the rate of 2.0 mLmin-1. After the filtration, the oncentration of the polyphenolsolution increased from 25.47% to 74.89%, the IC50on the enzyme inhibition reduced from 179.21 gmL-1 to 72.35 gmL-1, and the anti-enzymatic efficacy increased 2.47 times.
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