太子参中芜菁花叶病毒的RT-PCR检测

留言板

尊敬的读者、作者、审稿人, 关于本刊的投稿、审稿、编辑和出版的任何问题, 您可以本页添加留言。我们将尽快给您答复。谢谢您的支持!

姓名

邮箱

手机号码

标题

留言内容

验证码

基金项目:

福建省种业创新与产业化工程项目（2014S1477-12）;福建省自然科学基金项目（2015J01292）

RT-PCR Detection of Turnip Mosaic Virus on Pseudostellaria heterophylla

1.
Institute of Agricultural Bio-resources Fujian Academy of Agricultural Science, Fuzhou, Fujian 350003, China

摘要: 建立并应用太子参中芜菁花叶病毒的检测技术。采用RT-PCR技术，设计特定引物从太子参病株中的不同部位扩增特定片段，且进行序列分析和比对。序列比对结果表明，扩增得到的片段为芜菁花叶病毒外壳蛋白基因序列片段，本研究建立了一种简便可靠太子参中芜菁花叶病毒的检测方法，并且从太子参根、茎、叶和花中均检测到病毒目标序列。
- 太子参 /
- TuMV /
- RT-PCR
Abstract: A method to detect the turnip mosaic virus (TuMV) ondiseased Pseudostellaria heterophylla was established and applied. Specific genetic fragments at different locations in TuMV was amplified by RT-PCR, sequenced, and aligned. The sequence alignment indicated that the fragment was the coat protein (CP) gene of TuMV. Hence, a simple, reliable detection method based on the CP gene sequence of TuMV for the diagnosis on diseased P. heterophylla was established. Using the method, the target sequence was detected in the root, stem, leaf, and flower of P. heterophylla.
- Pseudostellaria heterophylla /
- turnip mosaic virus /
- RT-PCR

[1]	肖培根,李大鹏,杨世林.新编中药志:第一卷[M].北京:化学工业出版社,2002:191-193.
[2]	袁小坦,袁家雄.柘荣县太子参产业发展现状与对策[J].福建农业科技,2014,(7)::68-70.