Optimization of Chlorella vulgaris Flocculation Using Response Surface Methodology
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摘要: 以聚合氯化铝（Poly aluminium chloride，PAC）为絮凝剂，利用响应面法对普通小球藻Chlorella vulgaris的絮凝工艺进行优化。研究结果表明：影响C.vulgaris絮凝效率的主次因素顺序为絮凝时间>C.vulgaris初始OD值>PAC浓度；C.vulgaris的最佳絮凝工艺为：PAC浓度为57 mg·L-1，C.vulgaris初始OD值为2.06，絮凝时间为18 min。在此絮凝条件下，C.vulgaris的絮凝效率为95.82%。研究结果将为进一步研究C.vulgaris的絮凝采收工艺提供数据参考和技术支持。Abstract: Flocculation of Chlorella vulgaris using Poly aluminum chloride (PAC) was optimized by theresponse surface methodology. The factors that affected the flocculation efficiency were in the order flocculation duration>initial OD value of C.vulgaris>PAC concentration. The optimized flocculation conditions included a PAC concentration of 57 mg·L-1, an initial OD value of C.vulgaris of 2.06, and flocculation for 18 min to achieve a maximized efficiency of 95.82%.
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Key words:
- Chlorella vulgaris /
- Poly aluminum chloride /
- flocculation /
- response surface methodology
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图 1 PAC质量浓度对普通小球藻（Chlorella vulgaris）絮凝效率的影响
Figure 1. Effect of PAC concentration on flocculation efficiency
图 2 初始OD值对普通小球藻（Chlorella vulgaris）絮凝效率的影响
Figure 2. Effect of initial OD of C. vulgaris suspension on flocculation efficiency
图 3 絮凝时间对普通小球藻（Chlorella vulgaris）絮凝效率的影响
Figure 3. Effect of time on flocculation efficiency
图 4 小球藻初始OD值和絮凝时间的交互作用对絮凝效率的影响
Figure 4. Interactive effects of initial OD of C. vulgaris suspension and time on flocculation efficiency
图 5 PAC质量浓度和絮凝时间的交互作用对絮凝效率的影响
Figure 5. Interactive effects of PAC concentration and time on flocculation efficiency
图 6 小球藻初始OD值和PAC质量浓度的交互作用对絮凝效率的影响
Figure 6. Interactive effects of initial OD of C.vulgaris suspension and PAC concentration on flocculation efficiency
表 1 PAC絮凝C. vulgaris工艺的Box-Behnken试验因素水平设计
Table 1. Factors and levels for Box-Behnken experimental design on PAC flocculation of C. vulgaris
水平 因素 PAC质量浓度/（mg·L-1）（X1） C. vulgaris初始OD值（X2） 絮凝时间/min（X3） -1 50 1.5 10 0 60 2 15 1 70 2.5 20 
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表 2 PAC絮凝普通小球藻（Chlorella vulgaris）工艺的响应面试验设计方案及结果
Table 2. Design and results of response surface experiment on PAC flocculation of C. vulgaris
试验序号 X1 X2 X3 絮凝效率/% 1 60 2 15 87.28 2 60 2 15 89.13 3 60 2.5 10 79.76 4 50 2 20 89.55 5 60 2 15 91.01 6 70 1.5 15 53.7 7 50 2 10 59.75 8 50 2.5 15 76.29 9 60 1.5 10 61.03 10 50 1.5 15 75.11 11 60 2 15 95.05 12 70 2 10 69.07 13 60 2.5 20 91.94 14 70 2.5 15 89.56 15 60 1.5 20 92.46 16 70 2 20 92.06 17 60 2 15 92.26
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表 3 PAC絮凝C. vulgaris工艺条件优化的回归方程的方差分析及其系数显著性检验
Table 3. Variance analysis and significance test on regression equation of response surface experiment for optimizing PAC flocculating C. vulgaris
方差来源 平方和 自由度 均方 F值 P值 显著性 模型 0.27 9 0.03 16.02 0.0007 ** X1 0.0002 1 0.0002 0.11 0.7527 X2 0.039 1 0.039 21.06 0.0025 ** X3 0.12 1 0.12 61.89 0.0001 ** X1X2 0.031 1 0.031 16.45 0.0048 ** X1X3 0.00123 1 0.00123 0.66 0.4439 X2X3 0.00903 1 0.00903 4.85 0.0636 X21 0.044 1 0.044 23.88 0.0018 ** X22 0.019 1 0.019 10.38 0.0146 * X23 0.00324 1 0.00324 1.74 0.2284 残差 0.013 7 0.00186 失拟项 0.00935 3 0.00312 3.39 0.1346 误差项 0.00368 4 0.00092 总和 0.28 16 R2=0.9537 注: * 表示有显著性差异（P<0. 05）；** 表示有极显著性差异（P<0. 01）。
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