9个新育甜玉米自交系遗传聚类和主要农艺性状配合力分析

张扬; 廖长见; 林建新; 卢和顶; 陈山虎

doi:10.19303/j.issn.1008-0384.2019.01.003

9个新育甜玉米自交系遗传聚类和主要农艺性状配合力分析

doi: 10.19303/j.issn.1008-0384.2019.01.003

福建省农业科学院作物研究所, 福建福州 350013

基金项目:

福建省科技计划项目——省属公益类科研院所基本科研专项 2017R1026-1

国家重点研发计划子课题 2018YFD0100106

国家重点研发计划子课题 2018YFD0200707-5

福建省特色旱作物品种选育工程技术研究中心闽科计[2017]13号

福建省财政专项——福建省农业科学院科技创新团队项目 STIT2017-2-6

福建省农业科学院科技创新项目 A2015-1

福建省农业科学院科技创新项目 PC2018-3

福建省农业科学院科技创新项目 AB2017-9

福建省农业科学院对外合作项目 DEC201821213

详细信息

作者简介:
张扬(1985-), 男, 硕士, 助理研究员, 主要从事玉米遗传育种及栽培技术研究(E-mail:zywheat@163.com)

通讯作者:
陈山虎(1962-), 男, 研究员, 主要从事玉米遗传育种及栽培技术研究(E-mail:ymfaas@163.com)

中图分类号: S513
计量
- 文章访问数: 1114
- HTML全文浏览量: 185
- PDF下载量: 41
- 被引次数: 0
出版历程
- 收稿日期: 2018-05-28
- 修回日期: 2018-09-20
- 刊出日期: 2019-01-28

Combining Ability on Main Agronomic Traits and Genetic Clustering of Nine New Breeds of Sweet Corns

Crop Research Institute, Fujian Academy of Agricutural Sciences, Fuzhou, Fujian 350013, China

摘要

摘要: 目的利用遗传聚类和配合力分析来了解新育成9个甜玉米自交系的遗传背景和配合力，为组配新品种提供科学依据。方法以TS01-1、BS02-1、GS132、AS67、SS1等5个白粒自交系为母本，闽甜系688、闽甜系G73、闽甜系197、X90-34等4个高配合力黄粒自交系为父本，按5×4不完全双列杂交设计组配20个杂交组合，并对其株高、穗位高、穗长、穗粗、穗行数、行粒数、百粒重、出籽率、带苞叶穗重和去苞叶穗重等10个性状的配合力进行研究。结果从去苞叶穗重的一般配合力值得出，供试甜玉米自交系的配合力大小顺序，白粒自交系为AS67 > GS132 > TS01-1 > BS02-1 > SS1，黄粒自交系为闽甜系688 > X90-34 >闽甜系G73 >闽甜系197。从特殊配合力值得出，杂交组合AS67×闽甜系688的特殊配合力值最大，实际测产产量也最高。从分子水平对9个亲本的亲缘关系进行聚类分析，将9个亲本分成黄粒和白粒两个群。结论黄、白粒自交系间杂交具有一定的杂种优势；聚类分析结果与试验设计的父母本划分一致。
- 甜玉米 /
- 白色籽粒 /
- 黄色籽粒 /
- 自交系 /
- 配合力 /
- 遗传聚类
Abstract: Objective Genetic backgrounds and combining abilities of 9 newly bred sweet corn inbred lines were studied. Method The inbred lines included 5 white sweet corn female parents P1 (i.e., TS01-1, BS02-1, GS132, AS67 and SS1) and 4 sweet corn male parents P2 (i.e., Mintianxi 688, Mintianxi G73, Mintianxi 197 and X90-34). They were cross-bred into 20 two-tone crosses by a 5×4 incomplete diallel design. The combining ability on 10 traits including plant height, ear location, panicle length, ear girth, ear row count, grains per row, hundred-grain weight, kernel ratio, panicle weight with and without husk, of each hybrid was determined. Result Based on the de-husk panicle weight, the general combining abilities of the white sweet corn inbred lines were in the order of AS67 > GS132 > TS01-1 > BS02-1 > SS1, and those of the yellow sweet corns Mintianxi 688 > X90-34 > Mintianxi G73 > Mintianxi 197. AS67×Mintianxi 688 exhibited the highest specific combining ability with the greatest actual yield among all. The genetic cluster analysis classified the 9 parents into yellow-and white-kernel groups. Conclusion The yellow-kernel and white-kernel sweet corn inbred lines crossed between the 9 parents showed superior heterosis, and the classifications were consistent between the parents and the experimental design.
- sweet corn /
- inbred lines /
- combining ability /
- genetic clustering /

HTML全文

图 1 9个自交系聚类分析

Figure 1. Neighbor-joining trees of 9 inbred lines based on genetic distance

下载: 全尺寸图片幻灯片

表 1 40对核心引物

Table 1. 40 pairs of core primers

编号 Number	标记 Marker
1	bnlg439w1
2	umc1335y5
3	umc2007y4
4	bnlg1940k7
5	umc2105k3
6	phi053k2
7	phi072k4
8	bnlg2291k4
9	umc1705w1
10	bnlg2305k4
11	bnlg161k8
12	bnlg1702k1
13	umc1545y2
14	umc1125y3
15	bnlg240k1
16	phi080k15
17	phi065k9
18	umc1492y13
19	umc1432y6
20	umc1506k12
21	umc1147y4
22	bnlg1671y17
23	phi96100y1
24	umc1536k9
25	bnlg1520k1
26	umc1489y3
27	bnlg490y4
28	umc1999y3
29	umc2115k3
30	umc1429y7
31	bnlg249k2
32	phi299852y2
33	umc2160k3
34	umc1936k4
35	bnlg2235y5
36	phi233376y1
37	umc2084w2
38	umc1231k4
39	phi041y6
40	umc2163w3

下载: 导出CSV

表 2 9个自交系10个主要性状配合力方差分析结果(F值)

Table 2. Analysis of variance on combining ability on yield traits of inbred corns (F-value)

变异来源 Variation source	自由度 Translation freedom	株高 Plant height	穗位高 Ear high	穗长 Panicle length	穗粗 Ear thick	穗行数 Ear rows	行粒数 Grains per row	百粒重 Hundred-grain weight,	出籽率 Kernel ratio	穗重(带苞叶) Panicle weight(with husk)	穗净重 Panicle weight
区组Block	2	3.791	42.569^*	0.674	0.036	2.467	3.791	5.958^**	0.007^**	0.001	0.021
杂交种Hybrids	19	22.697^**	238.348^**	15.079^**	0.171^**	6.018^**	22.697^**	0.267	0.003	0.035^**	0.039
P₁	4	4.368	151.977	31.028	0.373^*	4.167	4.367	1.917^**	0.003	0.026	0.028
P₂	3	0.999	156.923	3.206	0.076	4.867	0.999	0.037	0.003	0.008	0.013
P₁×P₂	12	34.232^**	287.495^*	12.731^**	0.128^**	6.922^**	34.232^**	0.141^*	0.003^*	0.045^**	0.049^*
误差Error	38	5.558	12.004	0.349	0.041	1.344	5.557	0.250	0.001	0.004	0.028
注：、分别表示差异达5%和1%显著水平。 Note: , ** respectively indicate differences significant levels of 5% and 1%.

下载: 导出CSV

表 3 9个自交系主要性状一般配合力(GCA)相对效应值

Table 3. Relative effect of general combining ability(GCA)on yield traits of 9 inbred lines

亲本 Parent	株高 Plant height	穗位高 Ear high	穗长 Panicle length	穗粗 Ear thick	穗行数 Ear rows	行粒数 Grains per row	百粒重 Hundred-grain weight	出籽率 Kernel ratio	穗重(带苞叶) Panicle weight(with husk)	穗净重 Panicle weight
T1	-5.47	2.71	-7.98	1.21	-0.92	-0.23	-1.89	-0.56	-4.81	1.05
T2	2.44	6.18	-7.61	4.12	4.59	-1.41	-1.48	3.50	-0.58	-5.60
T3	1.74	-1.92	4.69	0.44	-1.83	-1.25	9.20	-0.32	3.25	5.90
T4	-2.34	-5.96	10.53	-5.40	-3.67	2.81	-0.66	-2.99	4.27	4.97
T5	3.63	-1.01	0.37	-0.36	1.83	0.08	-5.17	0.37	-2.13	-6.32
M1	-0.32	1.41	-1.94	-1.06	-3.85	-1.06	0.16	2.34	-0.36	2.04
M2	-0.95	-5.69	0.17	1.14	3.49	0.44	-1.81	-2.20	2.10	-2.22
M3	-0.73	-0.05	3.17	-1.34	-0.92	0.50	-0.49	-0.63	-2.31	-3.67
M4	2.00	4.33	-1.40	1.26	1.28	0.13	2.13	0.49	0.57	3.85

下载: 导出CSV

表 4 20个组合主要性状特殊配合力(SCA)相对效应值

Table 4. Estimates on specific combining ability (SCA) on yield traits of 20 hybrids

组合 Hybridized combination	株高 Plant height	穗位高 Ear high	穗长 Panicle length	穗粗 Ear thick	穗行数 Ear rows	行粒数 Grains per row	百粒重 Hundred-grain weight	出籽率 Kernel ratio	穗重(带苞叶) Panicle weight(with husk)	穗净重 Panicle weight
T1×M1	3.03	-3.48	2.34	1.45	-3.49	5.98	1.89	-1.76	4.30	0.51
T1×M2	1.96	-7.34	-1.92	-3.17	0.18	-4.27	10.43	-2.32	-3.85	-0.85
T1×M3	-6.30	0.52	-2.17	0.62	-2.75	5.36	-10.59	3.06	-5.18	1.75
T1×M4	1.31	10.30	1.74	1.10	6.06	-7.08	-1.72	1.02	4.73	-1.41
T2×M1	-2.66	-4.66	-8.61	-3.68	-8.99	-12.53	-8.37	-5.35	-15.06	-13.48
T2×M2	0.22	-11.87	3.00	-0.91	-5.32	6.28	5.09	2.43	3.92	-17.55
T2×M3	3.76	5.81	2.87	5.95	6.42	5.28	8.70	-0.53	11.65	28.73
T2×M4	-1.32	10.72	2.74	-1.36	7.89	0.97	-5.42	3.45	-0.50	2.30
T3×M1	8.56	21.00	-5.42	-0.27	8.44	-6.13	-5.91	3.10	-8.24	-10.55
T3×M2	7.12	13.75	-16.53	6.69	4.77	-7.31	-0.66	3.94	-1.59	-1.76
T3×M3	-6.80	-16.40	8.00	-3.84	-1.83	6.69	1.31	0.97	5.71	6.38
T3×M4	-8.88	-18.36	13.94	-2.58	-11.38	6.75	5.25	-8.01	4.13	5.94
T4×M1	-9.54	-2.10	8.92	1.06	6.61	7.94	2.30	4.38	13.36	11.78
T4×M2	-5.73	-1.59	9.68	0.36	-0.73	-1.69	-0.66	-4.51	3.71	8.69
T4×M3	9.24	5.11	-12.58	-5.39	-11.01	-15.19	2.96	-1.00	-16.88	-21.55
T4×M4	6.03	-1.43	-6.02	3.97	5.14	8.94	-4.60	1.13	-0.19	1.09
T5×M1	0.61	-10.77	2.76	1.45	-2.57	4.73	10.10	-0.37	5.65	11.74
T5×M2	-3.57	7.04	5.76	-2.97	1.10	6.98	-14.20	0.46	-2.19	11.48
T5×M3	0.11	4.95	3.87	2.65	9.17	-2.14	-2.38	-2.50	4.71	-15.30
T5×M4	2.85	-1.23	-12.40	-1.13	-7.71	-9.58	6.49	2.41	-8.17	-7.92

下载: 导出CSV

参考文献(16)

[1]	赵猛, 刘志雄, 陈强.玉米自交系主要数量性状的配合力分析与利用[J].北方农业学报, 2016, 44(5):21-24. doi: 10.3969/j.issn.2096-1197.2016.05.04 ZHAO M, LIU Z X, CHEN Q.Analysis of combining ability and use of maize inbred lines[J]. Inner Mongolia Agricultural Science, 2016, 44(5):21-24.(in Chinese) doi: 10.3969/j.issn.2096-1197.2016.05.04
[2]	李潮海, 赵亚丽, 王小星, 等.玉米昌7-2近缘系遗传多样性及其配合力分析[J].河南农业大学学报, 2008, 42(2):150-154. http://d.old.wanfangdata.com.cn/Periodical/hennannydxxb200802006 LI C H, ZHAO Y L, WANG X X, et al.Genetic Diversity of Chang7-2 Related Inbred Lines of Maize and Analysis of Their Combining Ability[J]. Journal of Henan Agricultural University, 2008, 42(2):150-154.(in Chinese) http://d.old.wanfangdata.com.cn/Periodical/hennannydxxb200802006
[3]	王瑞莲, 王永行, 韩凤英, 等.几个玉米自交系材料的配合力分析[J].内蒙古农业科技, 2015, 43(5):88-91. doi: 10.3969/j.issn.1007-0907.2015.05.032 WANG R L, WANG Y H, HAN F Y, et al.Analysis on the Combining Ability of Several Maize Inbred Lines[J]. Inner Mongolia Agricultural Science And Technology, 2015, 43(5):88-91.(in Chinese) doi: 10.3969/j.issn.1007-0907.2015.05.032
[4]	袁克录, 李继强, 谢新学, 等.6个新育玉米自交系配合力测定[J].分子植物育种, 2016, 14(1)216-222. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=fzzwyz201601029 YUAN K L, LI J Q, XIE X X, et al.Determination of Combining Ability of 6 newly maize inbred lines[J]. Molecular Plant Breeding, 2016, 14(1)216-222.(in Chinese) http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=fzzwyz201601029
[5]	YANG Y, XU Y, SHAH T, et al.Comparison of SSRs and SNPs in Assessment of Genetic Relatedness in Maize[J]. Genetic, 2011, 139(8)1045-1054. doi: 10.1007/s10709-011-9606-9
[6]	刘东军, 张宏纪, 张举梅.91份俄罗斯玉米自交系的遗传多样性分析[J].核农学报, 2016, 30(11):2112-2118. doi: 10.11869/j.issn.100-8551.2016.11.2112 LIU D J, ZHANG H J, ZHANG, J M.Genetic Diversity Analysis of 91 Russian Maize Inbred Lines[J]. Journal of Nuclear Agricultural Sciences, 2016, 30(11):2112-2118.(in Chinese) doi: 10.11869/j.issn.100-8551.2016.11.2112
[7]	杨文鹏, 关琦, 杨留启, 等.贵州70份玉米自交系的SSR标记遗传多样性及其杂种优势群分析[J].植物遗传资源学报, 2011, 12(2):241-248. http://d.old.wanfangdata.com.cn/Periodical/zwyczyxb201102012 YANG W P, GUAN Q, YANG L Q, et al.Genetic Diversity and Heterotic Group of 70Maize Inbred Lines in Guizhou SSR Markers[J]. Plant of Journal Genetic Resources, 2011, 12(2):241-248.(in Chinese) http://d.old.wanfangdata.com.cn/Periodical/zwyczyxb201102012
[8]	李新海, 袁力行, 李晓辉, 等.利用SSR标记划分70份我国玉米自交系的杂种优势[J].中国农业科学, 2003, 36(6):622-627. doi: 10.3321/j.issn:0578-1752.2003.06.004 LI X H, YUAN L X, LI X H, et al.Heterotic Grouping of 70 Maize Inbred Lines by SSR Markers[J]. Scientia Agricultura Sinica, 2003, 36(6):622-627.(in Chinese) doi: 10.3321/j.issn:0578-1752.2003.06.004
[9]	赵旭, 方永丰, 王汉宁, 等.玉米SSR标记杂优类群划分及群体遗传结构分析[J].核农学报, 2013, 27(12):1828-1838. doi: 10.11869/hnxb.2013.12.1828 ZHAO X, FANG Y F, WANG H N.Genetic diversity analysis and Heterosis grouping of Maize Inbred Lines[J]. Journal of Nuclear Agricultural Sciences, 2013, 27(12):1828-1838.(in Chinese) doi: 10.11869/hnxb.2013.12.1828
[10]	韩晴, 沈雪芳, 陆卫平.20个鲜食玉米杂交种DNA指纹库的构建[J].上海农业学报, 2014, 30(1):36-39. doi: 10.3969/j.issn.1000-3924.2014.01.009 HAN Q, SHEN X F, LU W P.Construction of DNA Fingerprint Database of 20 Table Corn Hybrids[J]. Acta Agricalture Shanghai, 2014, 30(1):36-39.(in Chinese) doi: 10.3969/j.issn.1000-3924.2014.01.009
[11]	王兵伟, 覃嘉明, 黄安霞, 等.SSR分子标记分析60份玉米自交系的遗传多样性[J].西南农业学报, 2014, 27(4):1358-1362. doi: 10.3969/j.issn.1001-4829.2014.04.003 WANG B W, TANG J M, HUANG A X, et al.Analysis of Genetic Diversity by SSR Markers for 60 Maize Inbred Lines[J]. Southwest China Journal of Agricultural Sciences, 2014, 27(4):1358-1362.(in Chinese) doi: 10.3969/j.issn.1001-4829.2014.04.003
[12]	黎裕, 王天宇.我国玉米育种种质基础与骨干亲本的形成[J].玉米科学, 2010, 18(5):1-8. http://d.old.wanfangdata.com.cn/Periodical/ymkx201005001 LI Y, WANG T Y.Germplasm Base of Maize Breeding in China and Formation of Foundation Parents[J]. Journal of Maize Sciences, 2010, 18(5):1-8.(in Chinese) http://d.old.wanfangdata.com.cn/Periodical/ymkx201005001
[13]	王玉民, 尹大鹏, 张春宵, 等.利用SSR标记分析35份糯玉米种质的遗传多样性[J].玉米科学, 2014, 22(6):27-31, 35. http://www.cnki.com.cn/Article/CJFDTotal-YMKX201406006.htm WANG Y M, YI D P, ZHANG C X, et al.Genetic Diversity of 35 Waxy Maize Germplasms Based on SSR Markers[J]. Journal of Maize Sciences, 2014, 22(6):27-31, 35.(in Chinese) http://www.cnki.com.cn/Article/CJFDTotal-YMKX201406006.htm
[14]	张世煌.商业育种只需要两个杂种优势群[J].种子科技, 2014(7):7-8. http://d.old.wanfangdata.com.cn/Periodical/zzkj201407007 ZHANG S H.Commercial breeding requires only two heterotic groups[J]. Seed Science and Technology, 2014(7):7-8.(in Chinese) http://d.old.wanfangdata.com.cn/Periodical/zzkj201407007
[15]	林建新, 陈山虎, 廖长见, 等.甜玉米新品种闽甜6855的选育[J].福建农业学报, 2016, 31(11):1171-1174. doi: 10.19303/j.issn.1008-0384.2016.11.006 LIN J X, CHEN S H, LIAO C J, et al.Breeding a New Sweet Corn variety, Mintian 6855[J]. Fujian Journal of Agricultural Sciences, 2016, 31(11):1171-1174.(in Chinese) doi: 10.19303/j.issn.1008-0384.2016.11.006
[16]	廖长见, 张扬, 林建新, 等.甜玉米新品种'闽甜683'的选育[J].福建农业学报, 2018, 33(6):591-596. doi: 10.19303/j.issn.1008-0384.2018.06.009 LIAO C J, ZHANG Y, LIN J X, et al.Breeding of a New Sweet Corn Variety 'Min Tian 683'[J]. Fujian Journal of Agricultural Sciences, 2018, 33(6):591-596.(in Chinese) doi: 10.19303/j.issn.1008-0384.2018.06.009