矮壮素对小叶楠抗寒生理的影响

费越; 白志波; 牛学舫; 胡蝶; 刘刚

doi:10.19303/j.issn.1008-0384.2019.01.016

矮壮素对小叶楠抗寒生理的影响

doi: 10.19303/j.issn.1008-0384.2019.01.016

费越^1,,
白志波¹,
牛学舫²,
胡蝶¹,
刘刚^1, ,

1.
长江大学楠木种质资源评价与创新中心, 湖北荆州 434025
2.
河北省廊坊市时代广场管理处, 河北廊坊 065000

基金项目:

湖北省自然科学基金项目 2017CFB390

湖北省科技支撑计划项目 2013BBB24

详细信息

作者简介:
费越(1994-), 女, 硕士研究生, 研究方向:园林植物与观赏园艺(E-mail:763938468@qq.com)

通讯作者:
刘刚(1989-), 男, 硕士, 研究方向:园林植物种质资源评价与利用(E-mail:616003@yangtzeu.edu.cn)

中图分类号: S792.24
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- 被引次数: 0
出版历程
- 收稿日期: 2018-09-09
- 修回日期: 2018-10-19
- 刊出日期: 2019-01-28

Effect of Chlormequat on Cold-resistance of Phoebe microphylla

FEI Yue^1
,,
BAI Zhi-bo¹,
NIU Xue-fang²,
HU Die¹,
LIU Gang^{1
, ,}

1.
Center of Phoebe Germplasm Resources Evaluation and Innovation, Yangtze Uinivesity, Jingzhou, Hubei 434025, China
2.
Management Office of Times Square, Langfang, Hebei 065000, China

摘要

摘要: 目的探讨不同浓度矮壮素（chlormequat chloride，CCC）溶液对小叶楠幼苗叶片组织生理的影响，探究CCC对小叶楠幼苗的抗寒机制，为人工抚育小叶楠提供科学依据。方法以小叶楠（Phoebe microphylla）幼苗为材料，采取室内控温系统模拟低温胁迫条件，研究CCC处理对低温胁迫下小叶楠幼苗可溶性蛋白含量（SP）、丙二醛含量（MDA）、相对电导率（REC）、超氧化物歧化酶活性（SOD）、过氧化物酶活性（POD）以及过氧化氢酶活性（CAT）的影响。结果低温条件下喷施CCC可使小叶楠幼苗叶片中SP含量增加，喷施50 mg·L^-1 CCC时，SP含量最高；在0℃和-2.5℃条件下，喷施100 mg·L^-1 CCC可有效降低MDA含量；喷施50 mg·L^-1 CCC使小叶楠叶片REC值降低；在-2.5℃时，喷施100 mg·L^-1 CCC溶液的小叶楠幼苗SOD、POD和CAT活性最强。结论通过综合分析各抗寒指标，喷施100 mg·L^-1 CCC溶液时隶属函数评价最高，说明CCC能有效提高小叶楠的抗寒能力。
- 小叶楠 /
- 矮壮素 /
- 丙二醛 /
- 可溶性蛋白 /
- 保护酶系统
Abstract: Objective Effect of chlormequat application on low-temperature tolerance of Phoebe microphylla was studied. Method Under simulated conditions, seedlings of P. microphylla were subjected to low-temperature stress with treatments of chlormequat chloride (CCC) in different concentrations. The contents of soluble protein (SP) and malondialdehyde (MDA), as well as the relative electrical conductivity (REC) and the activities of superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT) of the seedlings were determined. Result After spraying with CCC solutions, SP in leaves increased and reached a maximum when 50 mg/L of CCC was applied; MDA reduced when 100 mg·L^-1 of CCC was applied at 0℃ or -2.5℃; REC declined at the presence of 50 mg·L^-1 of CCC; and, the activities of SOD, POD and CAT peaked at the spraying of 100 mg·L^-1 of CCC at -2.5℃. Conclusion Based on the above mentioned cold resistance indicators, the CCC application on the seedlings at 100 mg·L^-1 scored the highest among all tested treatments on the membership function evaluation. It was considered effective for improving the cold resistance of P. microphylla.
- Phoebe microphylla /
- chlormequat /
- malondialdehyde /
- soluble protein /
- protective enzymes

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图 1 不同温度条件下喷施不同质量浓度CCC对小叶楠的影响

注：A、B、C分别表示在不同温度条件下，施加0、50、100、150 mg·L^-1的CCC处理结果。A为0℃条件下，施加4种质量浓度的CCC处理结果；B为-2.5℃条件下，施加4种质量浓度的CCC处理结果；C为-5℃条件下，施加4种质量浓度的CCC处理结果。

Figure 1. Effects of CCC concentration on P. microphylla at low temperatures

Note: A indicates treatments of 0, 50, 100 and 150 mg·L^-1CCC spraying at 0℃; B, treatments at-2.5℃; C, treatments at-5℃.

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表 1 不同低温条件及矮壮素用量处理小叶楠的生理指标变化

Table 1. CCC applications on physiological indices of P. microphylla at low temperatures

CCC质量浓度 CCC mass concentration/ (mg·L^-1)	可溶性蛋白Soluble protein/(mg·g^-1)			丙二醛Malonaldehyde/(μmol·g^-1)			相对电导率Relative electrolytic leakage/%			超氧化物歧化酶Superoxide dismutase/(U·g^-1)			过氧化物酶Peroxidase/(U·g^-1·min^-1)			过氧化氢酶Catalase/(U·g^-1·min^-1)
CCC质量浓度 CCC mass concentration/ (mg·L^-1)	0℃	-2.5℃	-5℃	0℃	-2.5℃	-5℃	0℃	-2.5℃	-5℃	0℃	-2.5℃	-5℃	0℃	-2.5℃	-5℃	0℃	-2.5℃	-5℃
对照CK	2.02±0.19Ab	1.47±0.20Bc	1.43±0.19Bb	0.05±0.002Ab	0.04±0.002Bb	0.05±0.002Ab	1.22±0.10Aa	1.05±0.10ABab	0.86±0.10Ba	3459.41±340.29Ba	4349.65±338.17Aab	4591.30±343.49Aa	470.25±39.00Aa	378.30±40.51Bb	344.59±38.52Bb	0.71±0.09Bb	1.03±0.11Ab	0.78±0.10Bc
50	2.97±0.20Aa	2.27±0.23Ba	2.35±0.22Ba	0.04±0.001Ac	0.04±0.001Ab	0.04±0.001Ac	1.04±0.05Ab	0.95±0.05ABb	0.87±0.06Ba	2879.00±343.44Ba	3801.92±343.93Ab	4075.79±350.29Aab	343.11±37.46Bb	317.03±39.39Bb	445.72±38.73Aa	1.07±0.05Aa	0.87±0.06Bbc	1.04±0.05Aa
100	1.68±0.12Bc	2.03±0.14Aab	2.06±0.12Aa	0.03±0.002Bd	0.04±0.002Ab	0.03±0.002Bd	1.18±0.06Aa	1.10±0.06Aa	0.98±0.06Ba	3275.29±472.62Ba	4800.73±489.72Aa	3592.49±476.87Bb	398.23±44.43Bab	555.96±47.49Aa	473.28±45.51ABa	0.93±0.10Ba	1.26±0.10Aa	1.01±0.11Bab
150	1.91±0.17Bbc	1.73±0.17Bbc	2.32±0.19Aa	0.07±0.002Ba	0.08±0.002Aa	0.07±0.002Ba	1.15±0.06Aab	1.01±0.05Bab	0.95±0.06Ba	2770.21±491.53Ba	4397.98±452.31Aab	3383.06±460.08Bb	315.55±51.46Bb	367.55±53.43Bb	494.69±56.46Aa	0.65±0.05Bb	0.81±0.06Ac	0.86±0.07Abc
注：表中数据为平均值±标准误；大写字母表示相同矮壮素质量浓度不同温度处理之间差异显著(P < 0.05)，小写字母表示相同温度不同矮壮素质量浓度处理之间差异显著(P < 0.05)。 Note: the data in the table are average value ±standard error; the capital letters mean significant differences between treatments with the same dwarf concentration at different temperatures (P < 0.05), and the lowercase letters showed significant differences between the treatments at the same temperature and different concentrations of dwarfs (P < 0.05).

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表 2 外源CCC对小叶楠抗寒指标的隶属函数评价

Table 2. Membership function evaluation on cold resistance indicators of P. microphylla upon exogenous CCC applications

温度 Temperature /℃	质量浓度 Mass concentration /(mg·L^-1)	隶属函数值Membership function value
温度 Temperature /℃	质量浓度 Mass concentration /(mg·L^-1)	SP含量 SP content	MDA量 MDA content	REC	SOD活性 SOD activity	POD活性 POD activity	CAT活性 CAT activity	隶属函数平均值 Membership function average	排序 Sort
0	0	0.27	0.53	1.00	1.00	1.00	0.14	0.66	4
	50	1.00	0.78	0.00	0.14	0.17	1.00	0.52	6
	100	0.00	1.00	0.84	0.69	0.52	0.67	0.62	5
	150	0.17	0.00	0.58	0.00	0.00	0.00	0.13	12
-2.5	0	0.00	0.82	0.67	0.58	0.24	0.51	0.47	8
	50	1.00	1.00	0.00	0.00	0.00	0.18	0.36	9
	100	0.70	0.97	1.00	1.00	1.00	1.00	0.95	1
	150	0.31	0.00	0.39	0.59	0.22	0.00	0.25	11
-5	0	0.00	0.53	0.00	1.00	0.00	0.00	0.26	10
	50	1.00	0.85	0.16	0.57	0.68	1.00	0.71	3
	100	0.66	1.00	1.00	0.17	0.82	0.88	0.76	2
	150	0.95	0.00	0.73	0.00	1.00	0.33	0.50	7

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参考文献(29)

[1]	中国植物志编委会.中国植物志(第31卷)[M].北京:科学出版社, 1982:7-68. EDITORIAL COMMITTEE of CHINESE FLORA.Flora of China(Volume 31)[M]. Beijing: Science Press, 1982:7-68.(in Chinese)
[2]	SEMWAL D K, SEMWAL R B.Ethnobotany, Pharmacology and Phytochemistry of the Genus Phoebe(Lauraceae)[J]. Mini-Reviews in Organic Chemistry, 2013, 10(1):12-26. doi: 10.2174/1570193X11310010002
[3]	丁宝章, 王遂义.河南植物志[M].郑州:河南科学技术出版社, 1978. DING B Z, WANG S Y.Flora of Henan[M]. Zhengzhou:Henan Science and Technology Press, 1978.(in Chinese)
[4]	马永征, 何舒怀, 费永俊.湖北建始楠木植物群落研究[J].江西农业大学学报, 2017, 39(4):748－763. http://d.old.wanfangdata.com.cn/Periodical/jxnydxxb201704015 MA Y Z, HE S H, FEI Y J.A Study of Nanmu Community in Jianshi, Hubei Province[J]. Acta Agriculturae Universitatis Jiangxiensis, 2017, 39(4): 748-763.(in Chinese) http://d.old.wanfangdata.com.cn/Periodical/jxnydxxb201704015
[5]	袁婵璐, 费越, 周明芹, 等.长阳县楠木资源初步调查[J].西北林学院学报, 2017, 32(4):100-105. http://d.old.wanfangdata.com.cn/Periodical/xblxyxb201704017 YUAN C L, FEI Y, ZHOU M Q, et al.Preliminary Research on Ecology Features of Phoebes in Changyang Country, Hubei Provience[J]. Journal of Northwest Forestry University, 2017, 32(4): 100-105.(in Chinese) http://d.old.wanfangdata.com.cn/Periodical/xblxyxb201704017
[6]	马永征, 何舒怀, 唐巍, 等.3种楠属植物2年生苗生长规律及光合生理特性[J].江苏农业科学, 2017, 45(22): 122－125. http://d.old.wanfangdata.com.cn/Periodical/jsnykx201722032 MA Y Z, HE S H, TANG W, et al.Growth regularity and photosynthetic physiological characteristics of 2-year-old seedlings of three species of Phoebe Nees[J]. Jiangsu Agricultural Sciences, 2017, 45(22): 122-125.(in Chinese) http://d.old.wanfangdata.com.cn/Periodical/jsnykx201722032
[7]	刘金林, 王小德.2种楠木树种幼苗耐盐性综合评价[J].江苏农业科学, 2018, 46(7):148-152. http://d.old.wanfangdata.com.cn/Periodical/jsnykx201807036 LIU J L, WANG X D.Comprehensive evaluation of salt tolerance of seedlings of two species of Phoebes[J]. Jiangsu Agricultural Sciences, 2018, 46(7): 148-152.(in Chinese) http://d.old.wanfangdata.com.cn/Periodical/jsnykx201807036
[8]	王启燕, 韩德元, 张妹丽.矮壮素对番茄幼苗抗寒性的作用机制[J].北京农业科学, 1994, 12(3):21-23. http://www.cnki.com.cn/Article/CJFDTOTAL-BJNK403.006.htm WANG Q Y, HAN D Y, ZHANG M L.Mechanism of chlormequat on Cold Resistance of Lycopersicone sculentum seedlings[J]. Beijing Agricultural Sciences, 1994, 12(3): 21-23.(in Chinese) http://www.cnki.com.cn/Article/CJFDTOTAL-BJNK403.006.htm
[9]	吴小林, 姚月华, 方霞, 等.两种叶型楠木叶片表现特性及光合特性的比较分析[J].浙江林业科技, 2018, 38(3):30-35. http://www.cnki.com.cn/Article/CJFDTOTAL-ZJLK201803005.htm WU X L, YAO Y H, FANG X, et al.Phenotypic and Photosynthetic Properties of Leaves in Phoebe zhennan[J]. J Zhejiang For Sci Technol, 2018, 38(3): 30-35.(in Chinese) http://www.cnki.com.cn/Article/CJFDTOTAL-ZJLK201803005.htm
[10]	董妍君.不同肥料配比对小叶楠幼苗生长及生理特性的影响[D].南宁: 广西大学, 2016. http://cdmd.cnki.com.cn/Article/CDMD-10593-1016199159.htm DONG Y J.Research of the effects on growth and physiological characteristics of Phoebe microphylla seedlings under different proportion of fertilizer[D]. Nanning: Guangxi University, 2016.(in Chinese) http://cdmd.cnki.com.cn/Article/CDMD-10593-1016199159.htm
[11]	LI X F, SCHMID B, WANG F, et.al.Net Assimilation Rate Determines the Growth Rates of 14 Species of Subtropical Forest Trees[J]. Plos One, 2016, 11(3):e0150644. doi: 10.1371/journal.pone.0150644
[12]	贺笑, 庞春花, 张永清, 等.多效唑和矮壮素对藜麦幼苗生长的影响[J].河南农业科学, 2018, 47 (1):26-31. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=hnnykx201801005 HE X, PANG C H, ZHANG Y Q, et al.Effects of Soaking Seeds with Paclobutrazol and Chlorocholine Chloride on the Growth of Quinoa Seedlings[J]. Journal of Henan Agricultural Sciences, 2018, 47 (1): 26-31.(in Chinese) http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=hnnykx201801005
[13]	张飞, 王艳秋, 朱凯, 等.矮壮素施用对甜高粱品种抗倒及光合物质生产的影响[J].西南农业学报, 2015, 28(5):1972-1976. http://d.old.wanfangdata.com.cn/Periodical/xnnyxb201505022 ZHANG F, WANG Y Q, ZHU K, et al.Effect of Chlormequat Chloride Application on Lodging Character, Photosynthetic and Material Production of Sweet Sorghum[J]. Southwest China Journal of Agricultural Sciences, 2015, 28(5): 1972-1976.(in Chinese) http://d.old.wanfangdata.com.cn/Periodical/xnnyxb201505022
[14]	刘刚, 费永俊, 涂铭, 等.矮壮素和缩节胺对闽楠幼苗抗寒性的影响[J].湖北农业科学, 2015, 54(6):1403-1406, 1444. http://d.old.wanfangdata.com.cn/Periodical/hbnykx201506031 LIU G, FEI Y J, TU M, et al.Effects of CCC and DPC on the Cold Resistance of Phoebe bournei[J]. Hubei Agricultural Sciences, 2015, 54(6): 1403-1406, 1444.(in Chinese) http://d.old.wanfangdata.com.cn/Periodical/hbnykx201506031
[15]	何舒怀, 马永征, 费永俊.外源水杨酸对广东润楠抗寒性的影响[J].江苏农业科学, 2018, 46(8): 155-158. http://d.old.wanfangdata.com.cn/Periodical/jsnykx201808037 HE S H, MA Y Z, FEI Y J.Effects of exogenous Salicylic Acid on Cold Resistance of Machilus kwangtungensis[J]. Jiangsu Agricultural Sciences, 2018, 46(8): 155-158.(in Chinese) http://d.old.wanfangdata.com.cn/Periodical/jsnykx201808037
[16]	王学奎.植物生理生化实验原理和技术[M].第2版.北京:高等教育出版社, 2006. WANG X K.Principles and Techniques of Plant Physiology and Biochemistry Experiments[M]. The second edition.Beijing: Higher Education Press, 2006.(in Chinese)
[17]	孙兵, 宗卫, 李金玲, 等.CaCl₂和水杨酸对桢楠和闽楠抗寒性的影响[J].长江大学学报(自然科学版), 2016, 13(33):16-21. http://d.old.wanfangdata.com.cn/Periodical/cjdxxb-rkxb201633005 SUN B, ZONG W, LI J L, et al.Effect of CaCl₂ and Salicylic Acid on Cold Resistance of Phoebe zhennan and Phoebe bournei[J]. Journal of Yangtze University (Natural Science Edition), 2016, 13(33): 16-21.(in Chinese) http://d.old.wanfangdata.com.cn/Periodical/cjdxxb-rkxb201633005
[18]	陶向新.模糊数学在农业科学中的初步应用[J].沈阳农业大学学报, 1982(2):96-107. http://www.cnki.com.cn/Article/CJFDTOTAL-SYNY198202012.htm TAO X X.A Preliminary Application of Fuzzy Mathematics in Agricultural Science[J]. Journal of Shenyang Agricultural College, 1982(2):96-107.(in Chinese) http://www.cnki.com.cn/Article/CJFDTOTAL-SYNY198202012.htm
[19]	魏永胜, 梁宗锁, 山仑, 等.利用隶属函数值法评价苜宿抗旱性[J].草业学报, 2005, 22(6):33-36. http://www.cnki.com.cn/Article/CJFDTotal-CYKX200506008.htm WEI Y S, LIANG Z S, SHAN L, et al.Comprehensive evaluation on alfalfa drought-resistance traits by subordinate function values analysis[J]. Pratacultural Science, 2005, 22(6):33-36.(in Chinese) http://www.cnki.com.cn/Article/CJFDTotal-CYKX200506008.htm
[20]	王志国, 何德, 金洪, 等.矮壮素对黄瓜幼苗形态及生理指标的影响[J].山东农业科学, 2010, 6(40):40-42, 46. http://d.old.wanfangdata.com.cn/Periodical/shandnykx201006010 WANG Z G, HE D, JIN H, et al.Effects of Chlormequat on Morphological and Physiological Indices of Cucumber Seedlings[J]. Shandong Agricultural Sciences, 2010, 6(40): 40-42, 46.(in Chinese) http://d.old.wanfangdata.com.cn/Periodical/shandnykx201006010
[21]	杨丽丽, 褚凤杰, 杜国强, 等.几个葡萄砧木品种的抗寒性研究[C]//全国葡萄学术研讨会, 2011. YANG L L, CHU F J, DU G Q, et al.Study on cold resistance of several grape rootstocks[C]// National Grape Symposium, 2011.(in Chinese)
[22]	刘建福, 徐泽玮.矮壮素缓解杨梅酸雨胁迫效应[J].生态学杂志, 2010, 29(8):1548-1553. http://d.old.wanfangdata.com.cn/Periodical/stxzz201008013 LIU J F, XU Z W.Alleviative effects of chlorocholine chloride on acid rain stress to Myricarubracv.Dongkui[J]. Chinese Journal of Ecology, 2010, 29(8): 1548-1553.(in Chinese) http://d.old.wanfangdata.com.cn/Periodical/stxzz201008013
[23]	樊丁宇, 靳娟, 杨磊, 等.不同枣砧木1年生枝抗寒性研究[J].经济林研究, 2018, 36(2):195-199. http://d.old.wanfangdata.com.cn/Periodical/jjlyj201802029 FAN D Y, JIN J, YANG L, et al.Study on the cold resistance of annual shoots of different jujube rootstocks[J]. Nonwood Forest Research, 2018, 36(2): 195-199.(in Chinese) http://d.old.wanfangdata.com.cn/Periodical/jjlyj201802029
[24]	刘西平.低温胁迫下栾树幼苗衰老与膜脂质过氧化关系[J].西北林学学报, 1995, 10(4):72-75. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=QK199500529300 LIU X P.Relationship between senescence and membrane Lipid Peroxidation of Luan Tree seedlings under low temperature stress[J]. Journal of Northwest Forestry College, 1995, 10(4): 72-75.(in Chinese) http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=QK199500529300
[25]	陈少裕.脂膜过氧化对植物细胞的伤害[J].植物生理学通讯, 1991, 27(2): 84-90. http://www.cnki.com.cn/Article/CJFD1991-ZWSL199102001.htm CHEN S Y.Injury of Membrane Lipid Peroxidation to Plant Cell[J]. Plant Physiology Communication, 1991, 27(2): 84-90.(in Chinese) http://www.cnki.com.cn/Article/CJFD1991-ZWSL199102001.htm
[26]	王华, 王飞, 陈登文等.低温胁迫对杏花SOD活性和膜脂过氧化的影响[J].果树学报, 2000, 17(3):197-201. http://d.old.wanfangdata.com.cn/Periodical/gskx200003009 WANG H, WAMG F, CHEN D W, et al.Effects of Low Temperature Stress on SOD Activity and Membrane Dreoxidization of Apricot Flowers[J]. Journal of Fruit Science, 2000, 17(3): 197-201.(in Chinese) http://d.old.wanfangdata.com.cn/Periodical/gskx200003009
[27]	李学玲, 庞海颖, 牛东伟, 等.不同外源激素对李花抗寒性及相关生理指标的影响[J].北方园艺, 2017(11):17-22. http://d.old.wanfangdata.com.cn/Periodical/bfyany201711004 LI X L, PANG H Y, NIU D W, et al. Effect of Different Exogenous Hormones on Cold-resistant Ability and Related Physiological Indicators of Plum Flower[J]. Northern Horticulture, 2017(11): 17-22. (in Chinese) http://d.old.wanfangdata.com.cn/Periodical/bfyany201711004
[28]	彭昌操, 孙中海.低温锻炼期间柑桔原生质体SOD和CAT酶活性的变化[J].华中农业大学学报, 2000, 19(4):384-387. http://d.old.wanfangdata.com.cn/Periodical/hznydx200004022 PENG C C, SUN Z H.Changes of SOD and CAT Activities of Citrus Protoplast During Cold Acclimation[J]. Journal of Huazhong Agricultural University, 2000, 19(4): 384-387.(in Chinese) http://d.old.wanfangdata.com.cn/Periodical/hznydx200004022
[29]	CHRIS B, MARC V M, DIRK I.Superoxide dismutase and stress tolerance[J]. Annual Review of Plant Biology, 1992, 43(1):83-116. doi: 10.1146/annurev.pp.43.060192.000503