解淀粉芽胞杆菌FJAT-8754产纤维素酶和淀粉酶发酵动力学模型的构建

葛慈斌; 蓝江林; 刘波; 王凯; 郑雪芳

doi:10.19303/j.issn.1008-0384.2019.06.011

解淀粉芽胞杆菌FJAT-8754产纤维素酶和淀粉酶发酵动力学模型的构建

doi: 10.19303/j.issn.1008-0384.2019.06.011

福建省农业科学院农业生物资源研究所, 福建福州 350003

基金项目:

福建省自然科学基金项目 2017J01048

福建省财政专项——福建省农业科学院科技创新团队建设项目 STIT2017-1-11

详细信息

作者简介:
葛慈斌(1973-), 男, 硕士, 副研究员, 研究方向:农业微生物(E-mail:benci2000@sohu.com)

通讯作者:
刘波(1957-), 男, 博士, 研究员, 研究方向:微生物生物技术(E-mail:fzliubo@163.com)

中图分类号: Q938
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- 被引次数: 0
出版历程
- 收稿日期: 2018-12-11
- 修回日期: 2019-03-25
- 刊出日期: 2019-06-28

Kinetics of Cellulase and Amylase-producing Fermentation of Bacillus amyloliquefaciens FJAT-8754

Institute of Agricultural Bio-resources, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian 350003, China

摘要

摘要: 目的解淀粉芽胞杆菌菌株FJAT-8754是一株高产纤维素酶、淀粉酶的芽胞杆菌，具有在复合饲料酶制剂生产中的应用潜力。为深入了解该菌株的发酵进程，以便实现对发酵过程进行最优化控制。方法采用50 L发酵罐发酵菌株FJAT-8754，分析发酵过程中菌体数量、pH值、底物浓度、纤维素酶和淀粉酶等的动态变化，并选用Logistic方程和Luedeking-Piret方程构建发酵动力学模型。结果根据菌株FJAT-8754发酵过程中各参数变化动态建立了模拟菌体生长、纤维素酶与淀粉酶产生和底物消耗的动力学模型，并应用SPSS 16.0软件对数据进行计算与分析，Origin 9.0软件经非线性拟合与优化，获得了最佳模型参数值。结论所构建的动力学模型的计算值与实验值拟合良好，较好地反映了菌株FJAT-8754发酵过程中菌体生长、底物消耗和酶合成的动力学特征。菌株FJAT-8754发酵过程中产物的合成属于生长部分偶联型。
- 解淀粉芽胞杆菌 /
- 发酵 /
- 动力学模型 /
- 纤维素酶 /
- 淀粉酶
Abstract: Objective To construct a kinetic model for the optimized fermentation of Bacillus amyloliquefaciens FJAT-8754, which was capable of effectively producing cellulase and amylase. Method A 50 L fermenter experiment was conducted to study the cellular growth, pH value, enzyme production and carbohydrate consumption of the fermentation and for subsequent process optimization. A kinetic model was established using the logistic and Luedeking-Piret equations, and experimental data calculated and analyzed with SPSS 16.0 software. Result The optimal fermentation conditions fitted well in the non-linear model as shown by Origin 9.0 software. Conclusion The kinetic model obtained accurately reflected the performance of the fermentation. The cellulase and amylase were products of FJAT-8754 metabolism in the culture.
- Bacillus amyloliquefaciens /
- fermentation /
- kinetic model /
- cellulase /
- amylase

HTML全文

图 1 菌株FJAT-8754发酵过程中各参数的变化曲线

Figure 1. The paremeters curves of the fermentation process of strain FJAT-8754

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图 2 生长动力学模型拟合曲线

Figure 2. Fitting of observed and predicted data from kinetics model on cell growth

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图 3 纤维素酶合成动力学模型拟合曲线

Figure 3. Fitting of observed and predicted data from kinetics model on cellulase formation

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图 4 淀粉酶合成动力学模型拟合曲线

Figure 4. Fitting of observed and predicted data from kinetics model on amylase formation

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图 5 底物消耗动力学模型拟合曲线

Figure 5. Fitting of observed and predicted data from kinetics model on corn starch consumption

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表 1 菌株FJAT-8754发酵过程中各参数间的相关性

Table 1. Interrelations among FJAT-8754 fermentation parameters

参数 Parameter	菌体浓度 Bacteria concentration	纤维素酶活力 Cellulase activity	溶氧量 Dissolved oxygen	总糖浓度 Total sugar	pH	淀粉酶活力 Amylase activity	还原糖浓度 Reducing sugar
菌体浓度Bacteria concentration	-	0.99817^**	-0.69637^**	-0.996^**	0.2035	0.98729^**	0.95251^**
纤维素酶活力Cellulase activity	0.99817^**	-	-0.6724^*	-0.99724^**	0.22521	0.99043^**	0.94422^**
溶氧量Dissolved oxygen	-0.69637^**	-0.6724^*	-	0.64207^*	0.48767	-0.58943^*	-0.83207^**
总糖浓度Total sugar	-0.996^**	-0.99724^**	0.64207^*	-	-0.28083	-0.99669^**	-0.92472^**
pH	0.2035	0.22521	0.48767	-0.28083	-	0.34552	-0.06936
淀粉酶活力Amylase activity	0.98729^**	0.99043^**	-0.58943^*	-0.99669^**	0.34552	-	0.90313^**
还原糖浓度Reducing sugar	0.95251^**	0.94422^**	-0.83207^**	-0.92472^**	-0.06936	0.90313^**	-
注：在0.05水平上显著相关(双向)；在0.01水平上极显著相关(双向)。 Note：Significantly correlated at 0.05 level (bilateral); **Extremely significantly correlated at 0.01 level (bilateral).

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