从采自福建各地紫云英的根瘤中分离出具有一般根瘤菌特征的菌株18株,在对其进行初步细菌学特性鉴定的基础上,利用16S rDNA进行序列分析,确定其系统发育地位。鉴定结果为:YX、Z3、GZ与ZK1为中慢生根瘤菌属Mesorhizobium菌种,B3、XZ、M6与百脉根中生根瘤菌Mesorhizobiumloti可能为同一菌属的不同变种;ZLH2为黄单孢菌属Xanthomonas菌种;M3、M10、ZK7、ZQH、B4、B7为土壤杆菌属Agrobacterium菌种;B6、B5、B1、 Z1为根瘤菌属Rhizobium菌种。为了进一步考察各鉴定菌株的结瘤情况,利用琼脂试管法和水培法将部分菌株与紫云英闽紫品种(1号、5号、6号、7号)和80410411品系进行配对结瘤,研究结果不仅进一步阐明了紫云英根瘤菌的系统发育多样性,并且通过对比琼脂试管法和水培法在结瘤结果上的异同,为探究紫云英与不同根瘤菌结瘤能力的差异提供了一种简便可行的研究方法。
A total of 18 isolates with general characteristics of Rhizobium were isolated from root nodules of Chinese milk vetch from different areas of Fujian. 16S rDNA sequence analysis was conducted on these bacteria with the purpose of determine the phylogenetic analyses. The result showed that YX、Z3、GZ and ZK1 were Mesorhizobium; B3、XZ、M6 were Mesorhizobium loti or varieties, ZLH2 was Xanthomonas, M3、M10、B4、B7、ZK7 and ZQH were Agrobacterium, B6、B5、B1 and Z1 were Rhizobium. For the purpose of investigate the nodulation performance of the identified bacterial, water culture and agar tube culture were applied between the part of identified rhizobial striains and milk vetch Minzi 1、Minzi 5、Minzi 6、Minzi 7 and 80410411. This study not only further elucidate the diversity of Chinese milk vetch rhizobium, but also provides a simple and feasible method to explore the differences in the ability of different rhizobium nodulation by contrast the different of nodulation with the agar tube culture and water culture.