电场处理对卷荚相思种子萌发特性的影响

朱梦恬; 江瑞熠; 张莹; 邓智文; 荣俊冬; 郑郁善; 陈礼光

电场处理对卷荚相思种子萌发特性的影响

1.
福建农林大学　林学院，福建　福州　350002

基金项目: 福建省科技计划区域发展项目（2015N3015）；福建省科技创新团队项目（闽教科[2018]49号）

详细信息

作者简介:
朱梦恬（1993 —），女，硕士研究生，研究方向森林培育技术，E-mail：870121549@qq.com

通讯作者:
陈礼光（1974 —），男，副教授，博士，研究方向森林培育技术，E-mail：fjclg@qq.com

中图分类号: S722.3
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出版历程
- 收稿日期: 2023-10-27
- 修回日期: 2023-12-16
- 网络出版日期: 2024-03-28

Effects of electric field treatment on seed germination characteristics of Acacia cincinnata

1.
College of Forestry, Fujian Agriculture and Forestry University, Fuzhou, Fujian　350002, China

摘要

摘要: 目的分析不同电场、时间及其两者交互处理对种子萌发特性的影响，为种子萌发及可持续经营提供理论参考。方法以卷荚相思种子为研究对象，采用室内模拟试验，以不同电压强度和处理时间为控制因素，电压强度为0～1.0 kV·cm⁻¹，时间处理为0～60 min，研究不同电压强度和时间交互处理对其种子萌发特性的影响。结果随着电压强度增强，处理时间的延长，卷荚相思种子的发芽率、发芽势和发芽指数均呈现不同程度的变化趋势。其中，在处理时间15、30和60 min下发芽率随着电压强度的增强呈现先降后升再降的趋势，但在处理时间45 min下发芽率随着电压强度的增强呈现先升后降的趋势；而发芽势和发芽指数均在电压强度0.6～0.8 kV·cm⁻¹和处理时间45 min下处于最优状态，且与CK相比具有显著差异（P＜0.05）。经过多元非线性回归分析得出，不同电压强度与时间交互处理下的最优回归方程为：Y=7.73+135.23X₁+1.24X₂-86.16X₁²−0.00911X₂²−0.75X₁X₂（R²=0.81，P＜0.05）。聚类分析结果电场和时间交互环境处理对发芽指数有非常显著的影响。结论电场和时间两者交互处理对卷荚相思种子萌发特性具有一定的影响，发芽率在0.6 kV·cm⁻¹处理45 min最高，说明适宜电压强度和处理时间可以提高卷荚相思种子发芽率。
- 卷荚相思 /
- 电压强度 /
- 种子萌发 /
- 聚类分析 /
- 非线性回归分析
Abstract: : Objective Physical technology is beneficial to the growth of plants and has a good application prospect. The effects of different electric fields, time and their interaction on seed germination characteristics were analyzed to provide theoretical reference for seed germination and sustainable management. Method This study used Acacia cincinnata seeds as the research object, using indoor simulation experiments, with different voltage intensities and treatment time as control factors, voltage intensities were 0～1.0 kV·cm⁻¹, and time treatments were 0～60 min to study the effects of different voltage intensities and time interactions on seed germination characteristics. Result The results showed that with the increase of voltage intensity and treatment time, the germination rate, germination potential and germination index of A.cincinnata seeds showed varying degrees of change. Among them, the germination rate decreased first, then increased and then decreased with the increase of voltage intensity at 15 min, 30 min and 60 min, but the germination rate increased first and then decreased with the increase of voltage intensity at 45 min. The germination potential and germination index were the best under the voltage intensity of 0.6 ～ 0.8 kV·cm⁻¹ and the treatment time of 45 min, and there was a significant difference compared with CK ( P＜0.05 ). After nonlinear regression analysis, the optimal regression equation under different voltage intensity and time interaction was: Y=7.73+135.23X₁+1.24X₂−86.16X₁²−0.00911X₂²-0.75X₁X₂ (R²=0.81, P＜0.05). Cluster analysis showed that the interaction of electric field and time had a very significant effect on germination index. Conclusion The interactive treatment of electric field and time had a certain effect on the germination characteristics of A.cincinnata seeds. The germination rate was the highest at 0.6 kV · cm⁻¹ for 45 min, indicating that the appropriate voltage intensity and treatment time could improve the germination rate of A. cincinnata seeds.
- Acacia cincinnata /
- Voltage intensity /
- Seed germination /
- Cluster analysis /
- Nonlinear regression analysis

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图 1 不同电压强度与时间交互处理下的聚类分析

1. 热图中红色表示电压对指标有促进作用，且颜色越深，作用越强；2. t1-0.2、t2-0.2、t3-0.2、t4-0.2代表0.2 kV/cm，t1-t4分别代表处理时间为15、30、45和60 min，后面以此类推；3. GE，发芽率；GR，发芽势；GI，发芽指数。

Figure 1. Clustering Analysis under Different Voltage Intensity and Time Interaction Red in the heat map indicates that the interaction of voltage and time promotes the index, and the darker the color, the stronger the effect.

1. Red in the heat map indicates that the voltage promotes the index, and the darker the color, the stronger the effect; 2. t1-0.2, t2-0.2, t3-0.2 and t4-0.2 represented 0.2 kV/cm, and t1-t4 represented treatment time of 15, 30, 45 and 60 min, respectively. And so on later; 3. GE, germination rate; GR, germination potential; GI, germination index.

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表 1 不同电压强度与时间交互处理对卷荚相思种子发芽率的影响

Table 1. Effect of different voltage intensity and time interaction treatment on seed germination rate of A. cincinnata

电压强度 Dielectric strength/kV·cm⁻¹	处理时间 Handling time/min
电压强度 Dielectric strength/kV·cm⁻¹	0	15	30	45	60
0.0	66.00±4.00 aA	66.00±4.00 aA	66.00±4.00 aB	66.00±4.00 aC	66.00±4.00 aAB
0.2	66.00±4.00 aA	48.67±2.52 cC	55.00±2.00 bcD	66.00±3.00 aC	60.67±5.77 abB
0.4	66.00±4.00 aA	56.00±2.00 bB	67.33±1.15 aB	70.33±1.53 aAB	70.00±3.46 aA
0.6	66.00±4.00 bA	67.67±2.52 abA	71.33±3.06 abAB	75.00±3.00 aA	74.67±7.57 aA
0.8	66.00±4.00 cA	66.67±2.08 bcA	74.33±3.51 aA	71.33±2.52 abAB	70.00±2.00 abcA
1.0	66.00±4.00 aA	65.33±2.52 aA	60.33±3.51 abC	59.33±2.52 bD	50.00±2.00 cC
同一行中不同小写字母代表不同处理时间存在显著差异（P＜0.05），同一列中不同大写字母代表不同电压强度存在显著差异（P＜0.05）。下同 Different lowercase letters in the same row represent significant differences in different treatment times (P＜0.05), and different uppercase letters in the same column represent significant differences in different voltage intensities (P＜0.05). Same below

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表 2 不同电压强度与时间交互处理对卷荚相思种子发芽势的影响

Table 2. Effect of Different Voltage Intensity and Time Interaction Treatment on Germination Potential of A. cincinnata Seeds

电压强度Dielectric strength/ kV·cm⁻¹	处理时间Handling time/min
电压强度Dielectric strength/ kV·cm⁻¹	0	15	30	45	60
0.0	39.67±2.08 aA	39.67±2.08 aCD	39.67±2.08 aB	39.67±2.08 aB	39.67±2.08 aB
0.2	39.67±2.08 abA	30.51±1.47 cE	36.24±3.31 abB	40.38±3.19 aB	35.33±1.15 bBC
0.4	39.67±2.08 abA	37.99±1.98 cD	38.38±4.65 cB	42.43±5.00 abB	46.67±4.16 aA
0.6	39.67±2.08 bA	48.02±1.60 aA	49.99±4.52 aA	50.58±1.87 aA	49.00±3.00 aA
0.8	39.67±2.08 cA	47.33±3.88 bAB	50.37±1.46 abA	52.73±1.79 aA	47.33±3.05 bA
1.0	39.67±2.08 abA	42.98±3.61 aBC	41.26±3.85 aB	41.23±5.27 aB	33.19±2.71 bC

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表 3 不同电压强度与时间交互处理对卷荚相思种子发芽指数的影响

Table 3. Effects of different voltage intensity and time interaction on seed germination index of A. cincinnata

电压强度Dielectric strength/kV·cm⁻¹	处理时间Handling time/min
电压强度Dielectric strength/kV·cm⁻¹	0	15	30	45	60
0.0	8.12±0.40 aA	8.12±0.40 aB	8.12±0.40 aBC	8.12±0.40 aCD	8.12±0.40 aBCD
0.2	8.12±0.40 abA	6.21±0.41 cD	7.38±0.63 bC	9.25±1.01 aABC	7.79±0.51 bCD
0.4	8.12±0.40 abA	7.12±0.47 bC	8.74±0.77 aAB	8.63±0.79 aBC	9.15±0.36 aAB
0.6	8.12±0.40 bA	9.24±0.66 abA	9.83±0.71 aA	9.57±0.55 abAB	9.35±1.18 abA
0.8	8.12±0.40 cA	8.91±0.22 bcAB	9.67±0.55 abA	10.12±0.52 aA	8.85±0.47 bcABC
1.0	8.12±0.40 aA	8.16±0.37 aB	7.64±0.70 abBC	7.15±0.23 bD	7.09±0.33 bD

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表 4 电压和时间交互处理对卷荚相思种子形态指标影响的双因素方差分析

Table 4. Two-way ANOVA of the effects of voltage and time interaction on the morphological indexes of A. cincinnata seeds

参数 Parameter	电压强度 Dielectric strength/kV·cm⁻¹		处理时间 Handling time/min		电压强度处理时间 Dielectric strength/kV·cm⁻¹ Handling time/min
参数 Parameter	F值 F value	P值 P value	F值 F value	P值 P value	F值 F value	P值 P value
发芽率 GE	51.004	0.000	13.908	0.000	8.292	0.000
发芽势 GR	42.144	0.000	4.332	0.010	3.139	0.003
发芽指数 GI	27.919	0.000	7.365	0.000	4.294	0.000

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表 5 不同电压强度与时间交互处理对发芽率非线性回归模型

Table 5. Summary of nonlinear regression model of germination rate under different voltage intensity and time interaction treatment

R²	调整后R² Adjusted R²	方差分析Variance analysis						F
		平方和Square sum		df		均方Mean square
		回归 regression	残差 residual error	回归 regression	残差 residual error	回归 regression	残差 residual error
0.81	0.79	3251.04548	779.93786	5	54	650.2091	14.44329	45.01806
R²表示方程拟合度，F表示整个拟合方程的显著。 R² represents the fitting degree of the equation, and F represents the significance of the whole fitting equation.

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