铁皮石斛羟基肉桂酰基转移酶基因家族生物信息学及其表达分析

龙小琴; 聂聪; 何基泽; 田野; 李昕燃; 皮鑫瑶; 王万军; 朱乾坤

doi:10.19303/j.issn.1008-0384.2022.004.010

铁皮石斛羟基肉桂酰基转移酶基因家族生物信息学及其表达分析

Bioinformatics and Expression of Hydroxycinnamyl transferase Gene Family from Dendrobium officinale

摘要

摘要:
目的对铁皮石斛HCT（Hydroxycinnamoyl transferase）基因家族进行特征鉴定和表达模式分析，为研究铁皮石斛黄酮类化合物和石斛酚的生物合成奠定研究基础。
方法通过NCBI在线网站获得铁皮石斛的基因数据信息，利用生物信息学方法分析铁皮石斛DoHCT基因家族成员系统进化树、DoHCTs保守基序和保守结构域，并且预测DoHCT家族成员的蛋白二级和三级结构；同时利用逆转录PCR的方法分析DoHCTs在不同胁迫条件下以及在根茎叶中的差异表达情况。
结果铁皮石斛DoHCTs共有 8个家族成员(DoHCT 1~8)，保守结构域分析结果显示：DoHCT7~8属于转移酶超家族，DoHCT 1~6属于PLN02481超家族。该家族基因的表达可受到非生物胁迫不同程度的影响，其中高温条件下DoHCT家族基因均下调表达；组织表达检测结果显示除DoHCT7外，其余基因均在根茎叶中差异表达。
结论在全基因组水平对DoHCT家族进行了系统的生物信息学分析，显示DoHCTs 在不同胁迫下以及铁皮石斛根茎叶中存在差异表达，证实其在非生物胁迫应答中起到调控作用。

Abstract:
Objective Characterization and the expressions of hydroxycinnamyl transferase (HCT) gene family from Dendrobium officinale were studied to reveal the biosynthesis of flavonoids and dendrophenol in the plant.
Method Relevant gene sequences were obtained from the NCBI online website. Conserved motifs and domains, evolutionary tree, and protein secondary and tertiary structures of each member of DoHCTs were analyzed using bioinformatics methods. Expressions of the genes under stresses and in roots, stems, and leaves were determined using RT-PCR.
Result The gene family had 8 members, DoHCT1–8. Their conserved domains showed that DoHCT7/8 belonged to the transferase superfamily and DoHCT1–6 to the PLN02481 superfamily. Abiotic stresses could affect the expressions, such as, elevated temperatures downregulated them. Except DoHCT7, the genes were differentially expressed in the roots, stems, and leaves.
Conclusion The comprehensive bioinformatics and expression analyses on the 8 members of the DoHCT family from D. officinale revealed that SoHCTs were with differental expression under different abiotic stress or tissues, and proved to play regulatory role in abiotic stress.

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