响应面法优化猪肚菇液体菌种发酵配方

郑泽杰; 施俊玲; 杨睿; 黄志龙; 赖淑芳; 胡开辉; 金文松; 蔡立君

响应面法优化猪肚菇液体菌种发酵配方

Response Surface-optimized Formulation for Culturing Pleurotus giganteus Spawns

摘要

摘要:
目的为优化猪肚菇液体菌种培养基配方、制备优良液体菌种、推动其工厂化生产快速发展，本研究旨在采用响应面法，以菌丝生物量为指标，探究不同碳源、氮源、无机盐种类及添加量对猪肚菇液体菌种生长的影响，旨在筛选最适合其液体菌种生长培养基配方。
方法试验使用三角摇瓶培养方法，以猪肚菇菌株ZD9为供试材料，进行培养基各营养成分的单因素试验，系统分析6种碳源、8种氮源、11种无机盐对其菌丝生长的影响；在此基础上，采用Box-Behnken响应面法优化并验证猪肚菇液体菌种生产的培养基配方。
结果猪肚菇液体菌种发酵培养至第6天时，菌丝生物量达到峰值，菌丝干重为1.33 g·100 mL⁻¹；适合其菌丝生长的营养要素为全麦粉、豆粕粉、MgSO₄·7H₂O及KCl，最优配方为全麦粉70 g·L⁻¹、豆粕粉27 g·L⁻¹、MgSO₄·7H₂O 2 g·L⁻¹、KCl 2 g·L⁻¹。该配方下的菌丝生物量实测值为2.51 g·100 mL⁻¹。
结论通过响应面法优化可获得适合猪肚菇液体菌种生长的培养基配方，经验证优化后的发酵配方较初始培养基菌丝生物量提高了89%，其菌丝生物量达到预期效果。

Abstract:
Objective Culture medium for growing Pleurotus giganteus spawns was formulated by means of response surface optimization.
Methods A single factor fermentation experiment was carried out in triangular shaking flasks to evaluate the effects of 6 carbon sources, 8 nitrogen sources, and 11 inorganic salts on mycelial growth of P. giganteus ZD9. Box-Behnken response surface design was used to optimize the medium formula.
Results The dry weight biomass of the spawns peaked at 1.33 g·100 mL⁻¹ on the 6^th day of culture in the optimized medium, which was formulated with the following ingredients at per liter rates of 70 g whole wheat flour, 27 g soybean meal powder, 2 g KCl, and 2 g MgSO₄·7H₂O to obtain a mycelial biomass at the concentration of 2.51 g·100 mL⁻¹.
Conclusion The optimized medium for culturing P. giganteus spawns achieved an 89% improvement on mycelial biomass production.

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