• 中文核心期刊
  • CSCD来源期刊
  • 中国科技核心期刊
  • CA、CABI、ZR收录期刊

近冰温贮藏下梗部保留与去除处理对西梅果实蔗糖代谢及品质的影响研究

Study on Sucrose Metabolism and Quality Changes of Prune with and without Stems under Near-Freezing Temperature Storage

  • 摘要:
    目的 探究近冰温(−1±0.5) ℃贮藏下梗部保留与去除对西梅果实蔗糖代谢以及对贮藏期果实品质的影响。
    方法 以‘法兰西’西梅为原料,将经预冷处理后的西梅分为带梗和脱梗两组,在近冰温(−1±0.5) ℃条件下贮藏120 d,定期取样测定西梅果实品质、可溶性糖类以及蔗糖代谢相关酶活性。
    结果 近冰温(−1±0.5) ℃条件下带梗贮藏能显著减缓西梅果实硬度下降,较好地延缓果实内部可溶性固形物(total soluble solids,TSS)、可滴定酸(titratable acid,TA)、抗坏血酸(ascorbic acid,AA)与可溶性糖类含量的下降。同时,与脱梗处理组相比,带梗处理有效提升了西梅果实酸性转化酶(acid invertase,AI)、中性转化酶(neutral invertase,NI)、蔗糖合成酶(sucrose synthase,SS)与蔗糖磷酸合成酶(sucrose phosphate synthase,SPS)活性。相关性分析表明,带梗组乙烯释放量、呼吸强度与果糖、葡萄糖含量及蔗糖代谢相关酶活性呈显著正相关,说明乙烯与呼吸代谢的增强可有效激活糖代谢进程,促进蔗糖向还原糖转化。
    结论 保留果梗贮藏可延缓西梅品质劣变,维持蔗糖代谢相关酶活性,为西梅贮藏保鲜及采后品质调控提供理论依据。

     

    Abstract:
    Objective Effects stem removal on sucrose metabolism and quality of post-harvest prunes in near-freezing temperature storage were studied.
    Method Pre-cooled ‘France’ Prune were divided into stem-removed and stem-retained groups and stored at (−1±0.5) ℃ for 120 d. Specimens were taken regularly to monitor soluble sugars content and sucrose metabolism-related enzyme activities in the fruit pulp as well as measure fruit firmness.
    Result Retaining the stem attached to a prune significantly slowed the softening of the fruit. It also delayed the decline on total soluble solids (TSS), titratable acid (TA), ascorbic acid (AA), and soluble sugars contents in the pulp. The activities of acid invertase (AI), neutral invertase (NI), sucrose synthase (SS), and sucrose phosphate synthase (SPS) were higher in the stem-attached fruits than the stem-removed counterparts. Ethylene release and respiratory intensity significantly correlated with the contents of fructose and glucose and the activities of sucrose metabolism-related enzymes of the prunes whether the stem was intact or being removed. The sugar metabolism process and conversion of sucrose to reducing sugars in the fruit in storage were allowed to remain active if the stem was not removed.
    Conclusion Near-freezing storage of post-harvest prunes with stems attached could effectively postpone quality decline and maintain sucrose metabolism-related enzyme activities of the fruits.

     

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