Abstract:
Objective Paenibacillus polymyxa NPDY05-8 is a beneficial bacterium that has certain control effects on wheat Fusarium head blight and corn stem rot. To understand its disease resistance mechanism, Genome sequencing technology was used to analyze the genome sequence of the strain, to explore its gene structure, to clarify its gene function, and to analyze its secondary metabolite, so as to provide basic clues for the utilization of the strain.
Methods the antimicrobial spectrum of the isolates was analyzed by the plate confrontation method, and the sequence and assembly were based on the Nonopore third-generation and illumina second-generation sequencing technology platforms, and the gene function was predicted and annotated.
Results bacillus Paenibacillus polymyxa NPDY05-8 showed antagonistic activity against 19 pathogenic fungi, the antimicrobial spectrumis between 62.00% and 92.00%.The total genome length was 5 132 538 bp and the G + C content was 45.63%, 5151 genes and 4 949 protein coding sequences were predicted, including a plasmid with a size of 226 178 bp. 3 902,3 489 and 1 391 genes were annotated in COG, GO and KEGG databases respectively. A total of 209 carbohydrate-related enzymes and 11 secondary metabolite gene clusters were also predicted.
Conclusion NPDY05-8 is rich in carbohydrate-related enzymes, including four cellulase genes, the synthetic Secondary
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