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灰树花不同浓度醇提物的活性成分分析及抗氧化、抗EV71病毒的作用研究

熊雯宇 何君强 汤丹琳 彭培植 江小琴 刘斌

熊雯宇,何君强,汤丹琳,等. 灰树花不同浓度醇提物的活性成分分析及抗氧化、抗EV71病毒的作用研究 [J]. 福建农业学报,2023,38(4):497−505 doi: 10.19303/j.issn.1008-0384.2023.04.014
引用本文: 熊雯宇,何君强,汤丹琳,等. 灰树花不同浓度醇提物的活性成分分析及抗氧化、抗EV71病毒的作用研究 [J]. 福建农业学报,2023,38(4):497−505 doi: 10.19303/j.issn.1008-0384.2023.04.014
XIONG W Y, HE J Q, TANG D L, et al. Active Ingredients and Antioxidative and Anti-EV71 Virus Effects of Grifola frondosa Ethanol Extract [J]. Fujian Journal of Agricultural Sciences,2023,38(4):497−505 doi: 10.19303/j.issn.1008-0384.2023.04.014
Citation: XIONG W Y, HE J Q, TANG D L, et al. Active Ingredients and Antioxidative and Anti-EV71 Virus Effects of Grifola frondosa Ethanol Extract [J]. Fujian Journal of Agricultural Sciences,2023,38(4):497−505 doi: 10.19303/j.issn.1008-0384.2023.04.014

灰树花不同浓度醇提物的活性成分分析及抗氧化、抗EV71病毒的作用研究

doi: 10.19303/j.issn.1008-0384.2023.04.014
基金项目: 中国博士后科学基金(2020M671918);福建省科学技术厅区域发展项目(2020N3002);福建省科学技术厅对外合作项目(2021I0008);福建省科技重大专项(2021NZ0101)
详细信息
    作者简介:

    熊雯宇( 1996−) ,女,硕士研究生.研究方向:食药用菌活性成分及功能营养(E-mail:1184503923@qq.com

    通讯作者:

    刘斌( 1966−) ,男,博士,教授,研究方向: 食品生物技术、分子营养学(E-mail:liubin618@ hotmail.com

  • 中图分类号: TS255.3

Active Ingredients and Antioxidative and Anti-EV71 Virus Effects of Grifola frondosa Ethanol Extract

  • 摘要:   目的  探究灰树花适宜的乙醇提取浓度,分析不同乙醇浓度提取对灰树花活性成分及抗氧化、抗EV71病毒活性的影响,促进灰树花的开发利用。  方法  分别采用55%、70%和95%的乙醇溶液作为提取溶剂对灰树花子实体进行超声波辅助提取,测定不同浓度乙醇提取物主要活性成分含量并对其抗氧化、抗EV71病毒能力进行相关性分析。  结果  不同乙醇浓度提取物活性成分含量存在差异,灰树花95%乙醇提取物(GFE95)中多酚和三萜含量最高分别为0.042%和1.82%,灰树花55%乙醇提取物(GFE55)中多糖和蛋白质含量最高,分别为33.08%和17.45%。GFE55、灰树花70%乙醇提取物(GFE70)和GFE95具有不同程度的抗氧化和EV71病毒抑制作用。其中,GFE95的抗氧化能力最好,对2,2-联苯基-1-苦基肼基(DPPH)、2,2′-联氨双二胺盐(ABTS+)、超氧阴离子(O2)和过氧化氢(H2O2)自由基的清除能力的IC50值分别为0.7427、0.7414、0.902、0.4146 mg·mL−1。GFE95的抗病毒作用较强,当样品质量浓度为200 μg·mL−1时,GFE95对EV71病毒的抑制率最高,达88.18%,其对EV71病毒抑制率的IC50值为194.80 μg·mL−1,TI值为9.65。皮尔逊相关性分析结果表明DPPH、ABTS+、O2自由基清除率与三萜类成分含量呈极显著正相关,相关系数分别为0.992、0.971和0.613(P<0.01)。O2、H2O2自由基清除率与多酚类成分含量呈极显著正相关,相关系数分别为0.921和0.997(P<0.01)。EV71病毒抑制作用与多酚、三萜类成分含量呈极显著正相关(P<0.01),相关系数分别为0.536和0.954。灰树花中三萜和多酚的含量与抗氧化、抗病毒活性具有较强的相关性。  结论  以体积分数为95%的乙醇溶液为提取剂,可有效提取灰树花中具有抗氧化、抗病毒活性的多酚、三萜、多糖等活性物质,提高灰树花的生物活性,是进一步促进灰树花的高值化应用开发的关键。研究结果为灰树花的提取溶剂选择及活性作用的研究奠定基础。
  • 图  1  灰树花不同浓度乙醇提取物中的活性物质含量

    不同字母表示处理间差异显著(P<0.05)。

    Figure  1.  Active ingredients in G.frondosa extracts obtained by using ethanol of varied concentrations

    Data with different letters indicate significant differences (P<0.05).

    图  2  灰树花不同质量浓度乙醇提取物的DPPH自由基清除率

    Figure  2.  DPPH free radical scavenging rates of G.frondosa extracts obtained by using ethanol of varied concentrations

    图  3  灰树花不同浓度乙醇提取物的ABTS+自由基清除率

    Figure  3.  ABTS+ free radical scavenging rates of G.frondosa extracts obtained by using ethanol of varied concentrations

    图  4  灰树花不同浓度乙醇提取物的O2自由基清除率

    Figure  4.  O2 free radical scavenging rates of G.frondosaextracts obtained by using ethanol of varied concentrations

    图  5  灰树花不同浓度乙醇提取物的H2O2自由基清除率

    Figure  5.  H2O2 free radical scavenging rates of G.frondosaextracts obtained by using ethanol of varied concentrations

    图  6  灰树花不同浓度乙醇提取物对Vero细胞的物质毒性

    与浓度0 μg·mL−1相比, **代表P<0.01;

    Figure  6.  Toxicity to Vero cells of G.frondosa extracts obtained by using ethanol of varied concentrations

    In comparison with 0 μg·mL−1; ** represents P<0.01.

    图  7  灰树花不同浓度乙醇提取物对EV71病毒的抑制率

    Figure  7.  Anti-EV71 activities of G.frondosa extracts obtained by using ethanol of varied concentrations

    表  1  灰树花不同浓度乙醇提取物抗氧化作用的IC50

    Table  1.   IC50 of antioxidative activities of G.frondosa extracts obtained by using ethanol of varied concentrations

    样品名称
    Description of sample
    DPPH自由基
    清除能力IC50
    DPPH·/( mg·mL−1)
    ABTS阳离子
    自由基清除能力IC50
    ABTS +·/( mg·mL−1)
    超氧阴离子
    自由基清除能力IC50
    O2·/( mg·mL−1)
    过氧化氢
    自由基清除能力IC50
    H2O2·/( mg·mL−1)
    GFE551.30700.93271.19601.0140
    GFE700.99580.80851.42802.5830
    GFE950.74270.74140.90260.4146
    下载: 导出CSV

    表  2  灰树花不同浓度乙醇提取物对EV71病毒的TC50 、TC50 和TI

    Table  2.   TC50, TC50, and TI on EV71 of G.frondosa extracts obtained by using ethanol of varied concentrations

    样品名称
    Description of
    sample
    样品半数
    中毒浓度
    TC50/(μg·mL−1)
    抗EV71病毒
    半数有效浓度
    IC50/(μg·mL−1)
    治疗指数
    TI
    GFE551868.00440.604.24
    GFE701869.00401.504.66
    GFE951879.00194.809.65
    下载: 导出CSV

    表  3  灰树花不同浓度乙醇提取物活性物质的含量与抗氧化、抗EV71病毒活性IC50的相关系数

    Table  3.   Correlation coefficients between IC50 of antioxidant and anti-EV71 activities and contents of active ingredients of G.frondosa extracts obtained by using ethanol of varied concentrations

    指标
    Index
    多酚
    Polyphenols
    三萜
    Triterpenoids
    多糖
    Polysaccharide
    蛋白质
    Protein
    DPPH自由基清除率IC50 IC50 of DPPH radical scavenging rate 0.130 0.992** −0.252 −0.997**
    ABTS+自由基清除率IC50 IC50 of ABTS+ radical scavenging rate 0.019 0.971** −0.358 −0.982**
    O2自由基清除率IC50 IC50 of O2 radical scavenging rate 0.921** 0.613** 0.706** −0.573**
    H2O2自由基清除率IC50 IC50 of H2O2 radical scavenging rate 0.997** 0.334 0.893** −0.286
    EV71病毒抑制率IC50 IC50 of EV71 virus inhibition rate 0.536** 0.954** 0.179 −0.937**
    *代表显著相关(P<0.05),**代表极显著相关(P<0.01)。
    * represents significant correlation (P<0.05); ** represents extremely significant correlation (P<0.01).
    下载: 导出CSV
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出版历程
  • 收稿日期:  2022-12-02
  • 修回日期:  2022-12-22
  • 网络出版日期:  2023-04-14
  • 刊出日期:  2023-04-28

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