Diversity of Bacillus and Fusarium Species in Rhizosphere Soil under Continuous Achyranthes bidentata Monoculture

CHEN Ting; WANG Juanying; WU Linkun; CHEN Jun; WU Hongmiao; LIN Sheng; LIN Wenxiong

doi:10.19303/j.issn.1008-0384.2020.11.009

Volume 35 Issue 11

Nov. 2020

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Article Navigation > Fujian Journal of Agricultural Sciences > 2020 > 35(11): 1234-1243

CHEN T, WANG J Y, WU L K, et al. Diversity of Bacillus and Fusarium Species in Rhizosphere Soil under Continuous Achyranthes bidentata Monoculture [J]. Fujian Journal of Agricultural Sciences，2020，35（11）：1234−1243 doi: 10.19303/j.issn.1008-0384.2020.11.009

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CHEN T, WANG J Y, WU L K, et al. Diversity of Bacillus and Fusarium Species in Rhizosphere Soil under Continuous Achyranthes bidentata Monoculture [J]. Fujian Journal of Agricultural Sciences，2020，35（11）：1234−1243 doi: 10.19303/j.issn.1008-0384.2020.11.009

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Diversity of Bacillus and Fusarium Species in Rhizosphere Soil under Continuous Achyranthes bidentata Monoculture

doi: 10.19303/j.issn.1008-0384.2020.11.009

CHEN Ting^{1, 2
,},
WANG Juanying^{1, 2},
WU Linkun^{1, 2},
CHEN Jun^{1, 2},
WU Hongmiao^{1, 2},
LIN Sheng^{1, 2},
LIN Wenxiong^{1, 2
,
,}

1.
College of Life Science, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China
2.
Fujian Provincial Key Laboratory of Agroecological Processing and Safety Monitoring, Fuzhou, Fujian　350002, China

Received Date: 2020-07-28
Rev Recd Date: 2020-09-27

Available Online: 2020-11-13

Publish Date: 2020-11-30

Abstract

Abstract

Objective Changes on the key microbial communities in the continuously cropped rhizosphere soil (CCRS) of Achyranthes bidentata Blume were studied to analyze the allelopathic effect for operational improvement on cultivation of the medicinal plants. Method The community structures of Bacillus spp. and Fusarium spp. in the rhizosphere soil of A. bidentata under 1, 10, and 15 years of consecutive monoculture were determined using denaturing gradient gel electrophoresis (DGGE). Soil sample from a virgin land was used as control (CK). Result Abundant Bacillus spp. dominated by B. stubtilis and B. cereus were isolated from the CCRS specimens. The CCRS under 10-year (10Y) and 15-year (15Y) of continuous cropping had a greater relative content of B. subtilis and B. halodurans than their 1-year (1Y) counterpart. With respect to Fusarium spp., the number of bands and species were relatively few, the community structure tended to be similar between CK and 15Y as well as between 1Y and 10Y, and F. solani and F. oxysporum being the dominant species. The qPCR results also indicated that continuous monoculture of A. bidentata increased the abundance of the beneficial bacteria but kept that of the harmful Fusarium spp. largely unchanged. Conclusion Continuous cropping A. bidentata enhanced the diversity and abundance of the beneficial Bacillus spp. without encouraging the expansion of the pathogenic Fusarium community in the rhizosphere. It appeared that the rhizosphere allelopathy under the cultivation practice had created a healthy ecosystem that promoted the growth of A. bidentata.
- Achyranthes bidentata Blume,
- continuous monoculture,
- Bacillus,
- Fusarium,
- DGGE

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References(35)

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