Expression and Correlation Analysis of Citrate Synthase Gene (<i>CS</i>) in Pomelo and Citric Acid Accumulation

LI Sitong; LI Zhenqin; LIN Wantong; WANG Junning

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LI S T, LI Z Q, LIN W T, et al. Expression and Correlation Analysis of Citrate Synthase Gene (CS) in Pomelo and Citric Acid Accumulation [J]. Fujian Journal of Agricultural Sciences，2024，39（2）：1−11

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LI S T, LI Z Q, LIN W T, et al. Expression and Correlation Analysis of Citrate Synthase Gene (CS) in Pomelo and Citric Acid Accumulation [J]. Fujian Journal of Agricultural Sciences，2024，39（2）：1−11

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Expression and Correlation Analysis of Citrate Synthase Gene (CS) in Pomelo and Citric Acid Accumulation

1.
Coastal Agricultural College of Guangdong Ocean University , Zhanjiang , Guangdong　524088, China

Received Date: 2023-10-11
Rev Recd Date: 2023-12-23

Available Online: 2024-03-28

Abstract

Abstract

: Objective Rganic acids are one of the important flavor substances in fruits, Citrate synthase (CS) plays a pivotal role as a rate-limiting enzyme in the organic acid metabolism of fruits. The citric acid synthase gene AheCS was cloned, and the citric acid content of jackfruit postharvest under different treatments was determined. The biological function of AheCS gene and the correlation between citric acid content and relative expression of AheCS gene were analyzed, and the possible role of AheCS gene in the metabolism of organic acids in jackfruit was discussed. Method Haida 2 jackfruit were utilized, treated with 0.5 mg·L⁻¹ 1-MCP and 1000 mg·L⁻¹ ETH (40%), and subjected to investigation into the dynamic changes in citric acid during jackfruit fruit ripening under room temperature (22±1°C) and 90% RH conditions. Three jackfruit CS genes (AheCS1, AheCS2, and AheCS3) were cloned, and their bioinformatics were analyzed. Its bioinformatics was analyzed, as well as AheCS1/2/3 under different post-harvest treatments (natural ripening, exogenous ethylene ripening, and 1-MCP delayed ripening). Result As jackfruit underwent natural ripening, citric acid content exhibited an initial increase followed by a decline. Exogenous ETH treatment accelerated the synthesis rate of citric acid, advancing the time of peak appearance. 1-MCP treatment inhibited the rate of citric acid decline in the early storage period, thus delaying the peak. Bioinformatics analysis revealed that the open reading frames (ORFs) of AheCS1/2/3 genes were 1422 bp to 1827 bp long. AheCS1/2/3 proteins contained the conserved 'WPNVDAHS' domain of citrate synthase, classifying them as members of the citrate synthase family. The amino acid sequences of AheCS1/2/3 showed the closest phylogenetic relationships with citrus CsCS (MH_048698.1), mulberry MnCs (XP010087965.1), and Anthurium AaCS (JAT55223.1), with similarities reaching 86.49%, 97%, and 86%, respectively. qRT-PCR results indicated that the expression of AheCS1/2/3 genes was low in the early stage of natural fruit ripening (CK) and increased in the later stage. Exogenous ETH treatment advanced the expression of AheCS1 and overall increased the expression of AheCS2/3. 1-MCP treatment delayed the increase in the expression of Peak occurrence time of AheCS1/2/3 but increased their expression in the later stages of maturity. Correlation analysis revealed a positive correlation between citric acid content and the expression of AheCS1/2/3 genes during jackfruit fruit ripening, with AheCS2 reaching a significant level. Conclusion The accumulation of citric acid during jackfruit maturation may be regulated by the AheCS2 genes, providing a foundation for further research on the functionality and genetic improvement of jackfruit AheCS genes.
- jackfruit,
- citrate acid,
- AheCS genes,
- cloning,
- gene expression

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References(42)

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