Identification and expression analysis of <i>YUCCA</i> gene family in <i>Passiflora edulis</i>

PAN Jiayi; PAN Ruoyun; JIANG Wenjie; REN Rui; FANG Ting

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PAN J Y, PAN R Y, JIANG W J, et al. Identification and expression analysis of YUCCA gene family in Passiflora edulis [J]. Fujian Journal of Agricultural Sciences，2024，39（2）：1−11

Citation:

PAN J Y, PAN R Y, JIANG W J, et al. Identification and expression analysis of YUCCA gene family in Passiflora edulis [J]. Fujian Journal of Agricultural Sciences，2024，39（2）：1−11

PAN J Y, PAN R Y, JIANG W J, et al. Identification and expression analysis of YUCCA gene family in Passiflora edulis [J]. Fujian Journal of Agricultural Sciences，2024，39（2）：1−11

Citation:

PAN J Y, PAN R Y, JIANG W J, et al. Identification and expression analysis of YUCCA gene family in Passiflora edulis [J]. Fujian Journal of Agricultural Sciences，2024，39（2）：1−11

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Identification and expression analysis of YUCCA gene family in Passiflora edulis

1.
Institute of Horticultural Plant Genetics and Breeding, Fujian Agriculture and Forestry University, Fujian Fuzhou,　350002

Received Date: 2023-09-11
Rev Recd Date: 2023-11-13

Available Online: 2024-03-28

Abstract

Abstract

Objective YUCCA gene encodes flavin monooxygenase, which is one of the main rate-limiting enzyme genes in the biosynthesis of Indole-3-acetic acid (IAA), and plays an important role in regulating plant growth and development. In this study, bioinformatics methods were used to identify members of the YUCCA gene family in Passiflora edulis Sims. In order to reveal the function of YUCCA family genes in hormone response, and provide references for bioinformatics studies of YUCCA family genes in other species. Methods The physicochemical properties and conserved domains of the encoded proteins, chromosome location, gene structure, phylogenetic tree and cis-acting elements of the genes were analyzed. qRT-PCR was used to explore the expression of some members treated with exogenous hormone IAA. Results A total of 29 YUCCA family members were identified in the passion fruit genome, unevenly distributed in 8 chromosomes. YUCCA gene structure analysis of passion fruit showed significant differences in the length of different genes, containing 1～8 introns and 8 conserved motifs. Through phylogenetic tree analysis, it was found that the YUCCA gene family of passion fruit can be divided into three distinct categories, and the YUCCA genes clustered in the same classification have high conservation. It was also found that the YUCCA gene of passion fruit was more closely related to alfalfa (Medicago sativa L.) and Arabidopsis thaliana, but more closely related to rice (Oryza sativa L.). Cis-acting element analysis showed that the promoter of YUCCA gene family of passion fruit was induced by various hormones and could respond to various stresses. Transcriptome data analysis showed that PeYUCCA6, PeYUCCA11 and PeYUCCA16 showed low or no expression in the leaves of Tainon and golden passion fruit, and the expression of PeYUCCA23 was the highest in Tainon and golden passion fruit, suggesting that this gene had a high effect on the development of passion fruit leaves. The results of qRT-PCR analysis showed that the expressions of PeYUCCA7, PeYUCCA13, PeYUCCA17, PeYUCCA24 and PeYUCCA26 genes were significantly increased after 100 μmol·L-1 IAA treatment. Conclusion The expression of YUCCA gene family members varies greatly under IAA treatment. YUCCA gene may play an important role in the growth and development of passion fruit and the resistance to adverse environment under growth regulator IAA.
- passion fruit,
- IAA,
- YUCCA gene family,
- Bioinformatics,
- Quantitative analysis

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References(29)

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